Early proliferation of umbilical cord blood cells from premature neonates

Luzo, Ângela Cristina Malheiros; Duarte, Adriana da Silva Santos; Salles, Tereza S.I.; Queiroz, Mary L.S.; Lorand-Metze, Irene; Costa, FF; Saad, Sara Teresinha Olalla

doi:10.1111/j.1423-0410.2007.00936.x

Cited by 7 publications

(7 citation statements)

References 58 publications

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“…These results do not support the viewpoint that CFUs are uncorrelated to fetal sex or that CFUs are related to gestational age. 8,13,24 Our results suggest that vaginal delivery and fetal weight have a positive influence on HQCBUs. We hypothesize that the distress response during vaginal delivery may increase cytokine levels, inducing primary cells to enter the fetal circulatory system and thus increasing the quantities of TNCCs, CD341 cells, and hematopoietic progenitor cells in CB.…”

Section: Discussionmentioning

confidence: 62%

“…Our results suggest that vaginal delivery and fetal weight have a positive influence on HQCBUs. We hypothesize that the distress response during vaginal delivery may increase cytokine levels, inducing primary cells to enter the fetal circulatory system and thus increasing the quantities of TNCCs, CD34+ cells, and hematopoietic progenitor cells in CB . Manegold and colleagues found that in the CB of women who gave birth via Cesarean section, cases with fetal distress had significantly higher TNCC, CD34+ cell count, and CFU‐GM compared to cases without fetal distress.…”

Section: Discussionmentioning

confidence: 99%

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Influence of maternal, infant, and collection characteristics on high‐quality cord blood units in Guangzhou Cord Blood Bank

Xie

et al. 2015

Transfusion

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We identified several factors that affect HQCBUs. These results may be used as a reference for updating CB collection strategies, with priority given to collecting CBUs from female fetuses older than 37 gestational weeks, at high birthweight, and born by vaginal delivery from mothers younger than 25 years of age, especially newborns with one parent carrying the trait or with meconium-stained amniotic fluid. The collected CBUs should be sent to the laboratory as soon as possible for priority processing, which will help to increase the number and ratio of HQCBUs and the effective use of CBB resources.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Influence of maternal, infant, and collection characteristics on high‐quality cord blood units in Guangzhou Cord Blood Bank

Xie

et al. 2015

Transfusion

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“…Of note, younger gestational age was associated with lower post‐TNCC, due to the smaller size of the baby and resulting smaller CBU collection, but the collections from these younger babies were enriched for both CFUs and CD34+ cell content reflecting higher potency overall. Samples of CB from preterm infants have been shown in vitro to have higher concentration of hematopoietic progenitor cells (i.e., CFUs) in addition to increased proliferative potential compared to term infants . Previous studies have also found that CBUs derived from donors of younger gestational age have higher CFU and/or CD34+ content .…”

Section: Discussionmentioning

confidence: 99%

Optimizing donor selection for public cord blood banking: influence of maternal, infant, and collection characteristics on cord blood unit quality

et al. 2013

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Background Banked unrelated donor umbilical cord blood (CB) has improved access to hematopoietic stem cell transplantation for patients without a suitably matched donor. In a resource-limited environment, ensuring that the public inventory is enriched with high-quality cord blood units (CBUs) addressing the needs of a diverse group of patients is a priority. Identification of donor characteristics correlating with higher CBU quality could guide operational strategies to increase the yield of banked high-quality CBUs. Methods Characteristics of 5267 CBUs donated to the Carolinas Cord Blood Bank, a public bank participating in the National Cord Blood Inventory, were retrospectively analyzed. Eligible CBUs, collected by trained personnel, were processed using standard procedures. Routine quality and potency metrics [post-processing total nucleated cell count (post-TNCC), CD34+, colony-forming units (CFUs)] were correlated with maternal, infant, and collection characteristics. Results High-quality CBUs were defined as those with higher post-TNCC (>1.25×109), and CD34+ + CFU in the upper quartile. Factors associated with higher CD34+ or CFU content included a shorter interval from collection to processing (<10 hours), younger gestational age (34–37 weeks; CD34++CFU) Caucasian race, higher birth weight (>3500grams) and larger collection volumes (>80ml). Conclusions We describe characteristics identifying high-quality CBUs, which can be used to inform strategies for CBU collection for public banks. Efforts should be made to prioritize collections from larger babies born before 38 weeks of gestation. CBUs should be rapidly transported to the processing laboratory. The lower quality of CBUs from non-Caucasian donors highlights the challenges of building a racially diverse public CB inventory.

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“…Similarly to cells isolated from umbilical cord blood (UCB) collected from premature neonate [49, 50] and to hAECs [44], oAECs obtained at early stage of gestation displayed a higher proliferative activity. As supposed for UCB derived cells, this aspect could be related to the larger amount of stem/progenitor cells, as well as, to a more rapid cell cycle required in preterm fetuses in order to drive an intense organogenesis [50].…”

Section: Discussionmentioning

confidence: 99%

“…As supposed for UCB derived cells, this aspect could be related to the larger amount of stem/progenitor cells, as well as, to a more rapid cell cycle required in preterm fetuses in order to drive an intense organogenesis [50]. The hypothesis of a larger aliquots of stem/progenitor cells in amniotic derived cells obtained from early amnia could be suggested by the higher expression of pluripotency markers, but not by the in vitro differentiation experiments.…”

Section: Discussionmentioning

confidence: 99%

Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties

Barboni

Russo

Curini

et al. 2014

Stem Cell Rev and Rep

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Stem cells isolated from amniotic epithelium (AECs) have shown great potential in cell-based regenerative therapies. Because of their fetal origin, these cells exhibit elevated proliferation rates and plasticity, as well as, immune tolerance and anti-inflammatory properties. These inherent attitudes make AECs well-suited for both allogenic and xenogenic cellular transplants in animal models. Since in human only at term amnion is easily obtainable after childbirth, limited information are so far available concerning the phenotypic and functional difference between AECs isolated from early and late amnia. To this regard, the sheep animal model offers an undoubted advantage in allowing the easy collection of both types of AECs in large quantity. The aim of this study was to determine the effect of gestational age on ovine AECs (oAECs) phenotype, immunomodulatory properties, global DNA methylation status and pluripotent differentiation ability towards mesodermic and ectodermic lineages. The immunomodulatory property of oAECs in inhibiting lymphocyte proliferation was mainly unaffected by gestational age. Conversely, gestation considerably affected the expression of surface markers, as well the expression and localization of pluripotency markers. In detail, with progression of gestation the mRNA expression of NANOG and SOX2 markers was reduced, while the ones of TERT and OCT4A was unaltered; but at the end of gestation NANOG, SOX2 and TERT proteins mainly localized outside the nuclear compartment. Regarding the differentiation ability, LPL (adipogenic-specific gene) mRNA content significantly increased in oAECs isolated from early amnia, while OCN (osteogenic-specific gene) and NEFM (neurogenic-specific gene) mRNA content significantly increased in oAECs isolated from late amnia, suggesting that gestational stage affected cell plasticity. Finally, the degree of global DNA methylation increased with gestational age. All these results indicate that gestational age is a key factor capable of influencing morphological and functional properties of oAECs, and thus probably affecting the outcome of cell transplantation therapies.

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Early proliferation of umbilical cord blood cells from premature neonates

Abstract: These results suggest that the higher capacity of proliferation and early differentiation of premature UCB might not be related only to the amount of stem/progenitor cells but also to a different timing of cell cycle entry.

Cited by 7 publications

References 58 publications

Influence of maternal, infant, and collection characteristics on high‐quality cord blood units in Guangzhou Cord Blood Bank

Influence of maternal, infant, and collection characteristics on high‐quality cord blood units in Guangzhou Cord Blood Bank

Optimizing donor selection for public cord blood banking: influence of maternal, infant, and collection characteristics on cord blood unit quality

Gestational stage affects amniotic epithelial cells phenotype, methylation status, immunomodulatory and stemness properties

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