Early protection against homologous challenge after a single dose of replication-defective human adenovirus type 5 expressing capsid proteins of foot-and-mouth disease virus (FMDV) strain A24

Venezuelan Equine Encephalitis Replicon Particles Can Induce Rapid Protection against Foot-and-Mouth Disease Virus

“…Ad5-GFP and an Ad5 vector containing a small interfering RNA (siRNA) directed against GFP (Ad5-siGFP) were produced using the pAd5-Blue direct ligation system (26). All vectors were purified as previously described and tested for foreign gene expression in IB-RS-2 cells (27).…”

Granulocyte-macrophage colony-stimulating factor does not increase the potency or efficacy of a foot-and-mouth disease virus subunit vaccine

“…Recombinant virus Ad5-pGMCSF was produced by transfection of 293 cells with PacI digested pAd5-pGMCSF following the protocol described (Wu et al, 2003b). The virus was isolated, propagated in 293 cells, and purified by CsCl gradient centrifugation (Mayr et al, 1999;Moraes et al, 2002).…”

“…Several approaches are being used to develop alternative FMD vaccines that address these concerns, including construction of modified live-virus , Almeida et al 1998, biosynthetic proteins (Kleid et al 1981, McKercher et al 1985, synthetic peptides (Bittle et al 1982, Clark et al 1983, Brown 1988, Blanco et al 2001, naked DNA vectors (Ward et al 1997, Chinsangaram et al 1998, Wong et al 2000, Benvenisti et al 2001, Cedillo-Barrón et al 2001, and recombinant viruses (Sanz-Parra et al 1999a,b, Mayr et al 1999, Berinstein et al 2000, Moraes et al 2002, Wu et al 2003b). However, most of these approaches have either been unsuccessful in both swine and cattle or require multiple inoculations to induce protection.…”

“…Human adenovirus (Ad5) has been used as a vector for FMD vaccines encoding the capsid (P1-2A) (Sanz-Parra et al 1999a,b) or P1-2A and 3C protease coding regions of FMDV (Mayr et al 1999, Moraes et al 2002, Wu et al 2003b. Inoculation with one dose of Ad5 containing the serotypes A24 Cruzeiro P1-2A and A12 3C coding regions (Ad5-A24) protected swine from direct inoculation challenge with homologous virus (Moraes et al 2002).…”

“…Inoculation with one dose of Ad5 containing the serotypes A24 Cruzeiro P1-2A and A12 3C coding regions (Ad5-A24) protected swine from direct inoculation challenge with homologous virus (Moraes et al 2002). More recently we have attempted to develop Ad5 vaccine vectors for other FMDV serotypes, in particular isolates of serotype O that are currently causing disease outbreaks throughout the world.…”