Early protooncogene expression during hemin-induced differentiation of human erythroleukemic cells

Griffin, M.; Nelson, John; Abraham, Nader G.

doi:10.1016/0145-2126(90)90113-n

Cited by 17 publications

(7 citation statements)

References 33 publications

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“…The results of the antisense inhicells, 7,8 cellular ETS-1 is expressed in immature hematopoietic cells of the erythroid lineage as well as in hematopoietic bition experiments argue that ETS-1 is essential for this response, and are also interesting considering the current tissues in the developing mouse, 30,31,58,59 and it has recently been reported that the MafB gene product binds to the DNA axiom that Ara-C induces an erythroid phenotype by inhibiting DNA replication, while hemin utilizes mechanisms binding domain of ETS-1 and inhibits erythroid differentiation of chicken hematopoietic progenitors. 60 It was suggested that which are different from those of Ara-C. [63][64][65][66][67] Our finding that ETS-1 expression contributes to the hemoglobinization in MafB is an interaction partner and repressor of ETS-1, and that is elevated expression inhibits erythroid differentiation. Our response to hemin or Ara-C treatment suggests an integral role for ETS-1 expression in erythroid differentiation in both findings are consistent with those observations and further support the hypothesis that the ETS-1 gene has a role in mechanisms.…”

Section: Discussionmentioning

confidence: 99%

ETS-1 induces increased expression of erythroid markers in the pluripotent erythroleukemic cell lines K562 and HEL

et al. 1997

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Members of the ETS gene family are known to be expressed in other members of the ETS gene family may have crucial roles hematopoietic tissues and cell lines, and there is increasing in specific aspects of hematopoietic cell development. evidence that ETS proteins may play a role in normal hemato-The c-ETS-1 gene, the cellular homolog to v-ets, is poietic cell development. We demonstrate that ETS-1 can conexpressed primarily as two major transcripts of 6.8 and 2.7 kb, virus with erythroleukemia and with the regulation of

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Section: Discussionmentioning

confidence: 99%

ETS-1 induces increased expression of erythroid markers in the pluripotent erythroleukemic cell lines K562 and HEL

et al. 1997

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“…The procedure used was a modification of that of Hafen and Levine (23); Wilcox, Gee and Roberts (24); and Griffin, Nelson and Abraham (25).…”

Section: Cellmentioning

confidence: 99%

“…In Situ Hybridization. The procedure used was a modification of that of Hafen and Levine (23); Wilcox, Gee and Roberts (24); and Griffin, Nelson and Abraham (25). [35S]labeled complementary DNA (cDNA) probes were prepared as described below.…”

Section: Nader G Abraham' and Shigeki Shibahara3mentioning

confidence: 99%

Coexpression of erythropoietin and heme oxygenase genes in Hep3B cells

et al. 1993

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Exposure of Hep3B cells to metalloporphyrins (tinprotoporphyrin and heme) or cobalt chloride resulted in the production of a significant number of heme oxygenase transcripts, erythropoietin transcripts or both, as indicated by in situ hybridization. Exposure to heme 10 mumol/L resulted in a 30-fold to 40-fold increase in cells expressing erythropoietin messenger RNA (erythropoietin-positive cells) by 6 hr; this increased level remained elevated for 24 hr. Tin-protoporphyrin (10 mumol/L) produced an eightfold to 10-fold increase in erythropoietin RNA within 40 min. This value then returned to control levels by 60 min. Exposure to cobalt chloride (100 mumol/L) resulted in a 20-fold to 30-fold increase in erythropoietin expression for 5 to 20 min, returning to control by 40 min. Additionally, nuclear runoff assays demonstrated that the increase in heme oxygenase or erythropoietin messenger RNA accumulation by cobalt chloride appeared to be a result of stimulated transcription of the heme oxygenase and erythropoietin genes. However, the pattern for heme oxygenase messenger RNA induction was different from that for erythropoietin expression. Heme produced an immediate expression of heme oxygenase RNA (50-fold within 5 min) and a second sustained response during the next 24 hr. Tin-protoporphyrin also produced an immediate response (40-fold within 5 min) and remained elevated (20-fold) for 6 hr. Cobalt chloride produced a 22-fold increase within 20 min and returned to the control value by 1 hr. Thus both erythropoietin and heme oxygenase genes appear to be expressed after treatment with tin-protoporphyrin, heme or cobalt chloride; however, the time and patterns of expression are different.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…More recently, Grifin et al [92] have shown the heme signal for differentiation may be coupled with specific changes in expression of c-myc and myb. Using HEL cells, we have also shown that hemin induced a transient increase in the myc mRNA after 1 to 3 hours, which precedes that of PBG-D and globin mRNA levels.…”

Section: Regulatory Esfect Of Heme On Oncogene and Growth Factor Recementioning

confidence: 99%

Eclectic mechanisms of heme regulation of hematopoiesis

Abraham

Levere

Lutton

1991

The International Journal of Cell Cloning

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Regulatory features of heme (ferroprotoporphyrin IX) on hematopoietic growth/differentiation and related processes are reviewed. It is emphasized that expressions of specific erythroid and nonerythroid heme biosynthetic and degradatory enzymes are required, and the regulatory processes whereby this occurs is considered. The specificity of heme, relationship to cellular events such as differentiation, response to growth factors, oncogene and receptor expression, and how heme counteracts toxic effects such as viral growth are all discussed. The significance of heme in the hemopoietic bone marrow microenvironment and growth factor network are considered. Finally, the third pathway for arachidonic acid metabolism via the heme-cytochrome P450 monooxygenase system, in addition to cyclooxygenase and lipoxygenase, by bone marrow adherent cells and its role in cellular differentiation is briefly reviewed.

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Early protooncogene expression during hemin-induced differentiation of human erythroleukemic cells

Cited by 17 publications

References 33 publications

ETS-1 induces increased expression of erythroid markers in the pluripotent erythroleukemic cell lines K562 and HEL

ETS-1 induces increased expression of erythroid markers in the pluripotent erythroleukemic cell lines K562 and HEL

Coexpression of erythropoietin and heme oxygenase genes in Hep3B cells

Eclectic mechanisms of heme regulation of hematopoiesis

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