Early Reactions of the Subcutaneous Tissue to Repeated Injections of Carcinogens in Aqueous Solutions

Hooson, Jean; Grasso, P.; Gangolli, S.D.

doi:10.1038/bjc.1971.64

Cited by 15 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This particular aspect of carcinogen treatment has also been noted by Vasiliev et al (1962), who demonstrated an inhibition of fibroblastic differentiation and growth around paraffin pellets containing carcinogens. Similar findings (Hooson et al, 1971(Hooson et al, , 1973 followed repeated injections of watersoluble carcinogens into the subcutis. Moreover, carcinogenic hydrocarbons inhibit the proliferation of fibroblasts in vitro, whilst chemically related but noncarcinogenic hydrocarbons do not (Vasiliev & Guelstein, 1963).…”

Section: Discussionsupporting

confidence: 57%

See 1 more Smart Citation

Cellular progression of neoplasia in the subcutis of mice after implantation of 3,4-benzpyrene

Westwood¹,

Longstaff²,

Butler³

1979

Br J Cancer

View full text Add to dashboard Cite

An implantation model has been used to investigate the cellular progression of chemically induced subcutaneous neoplasia in the mouse. Implantation of 3,4-benzpyrene induced persistent changes in the normal process of connective tissue formation around the implant. Light-microscope and autoradiographic studies have shown a temporal progression from aberrant filter- or muscle-associated cells through proliferative foci to large invasive sarcoma. Electron microscopy revealed that presarcomatous cell foci consisted of one of two different cell types. These were either spindle cells with ultrastructural characteristics similar to foreign-body-induced sarcoma, or cells with the ultrastructural features of rhabdomyosarcoma. The subsequent appearance of two histological groups of sarcoma that were ultrastructurally similar to the cells of the early proliferative foci indicated that both elements may progress to form tumours. However, the constituent cells of both groups of tumours displayed a broad histological and ultrastructural spectrum and the marked similarity between the undifferentiated cells of each suggested that both may have arisen from diverse differentiation of a common pluripotential cell such as the pericyte. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 Fig. 9

show abstract

Section: Discussionsupporting

confidence: 57%

“…This led to a profound distortion of normal fibrogenesis, and encapsulation of the implanted material. Similar responses were seen by Hooson et al (1971Hooson et al ( , 1973 after s.c. injection of water-soluble carcinogens.…”

supporting

confidence: 74%

Cellular progression of neoplasia in the subcutis of mice after implantation of 3,4-benzpyrene

Westwood¹,

Longstaff²,

Butler³

1979

Br J Cancer

View full text Add to dashboard Cite

show abstract

“…Leaving frank carcinogens aside, it was noted that the otherwise biologically inert materials which did lead to intense tissue reactions and injection site sarcomas were those which possessed surface activity, acidic pH or precipitated at the site of injection [103, 107–110]. …”

Section: Possible Etiologiesmentioning

confidence: 99%

Origins of Injection-Site Sarcomas in Cats: The Possible Role of Chronic Inflammation—A Review

Woodward¹

2011

ISRN Veterinary Science

View full text Add to dashboard Cite

The etiology of feline injection-site sarcomas remains obscure. Sarcomas and other tumors are known to be associated with viral infections in humans and other animals, including cats. However, the available evidence suggests that this is not the case with feline injection-site sarcomas. These tumors have more in common with sarcomas noted in experimental studies with laboratory animals where foreign materials such as glass, plastics, and metal are the causal agent. Tumors arising with these agents are associated with chronic inflammation at the injection or implantation sites. Similar tumors have been observed, albeit infrequently, at microchip implantation sites, and these also are associated with chronic inflammation. It is suggested that injection-site sarcomas in cats may arise at the administration site as a result of chronic inflammation, possibly provoked by adjuvant materials, with subsequent DNA damage, cellular transformation, and clonal expansion. However, more fundamental research is required to elucidate the mechanisms involved.

show abstract

“…Histological descriptions of the connective tissue reaction around silicone rubber implants (Nothdurft, 1961) and various plastic films (Oppenheimer et al, 1959) emphasize the importance of the formation of a thick, avascular connective tissue capsule, with consequent isolation of the fibroblasts, as an essential step in the evolution of malignancy.…”

Section: Tissue Reaction To Surfactant Colouringsmentioning

confidence: 99%

Injection Site Tumours and Preceding Pathological Changes in rats Treated Subcutaneously with Surfactants and Carcinogens

Hooson¹,

Grasso²,

Gangolli³

1973

Br J Cancer

Self Cite

View full text Add to dashboard Cite

Summary.-The sequential histological changes and neoplastic response occurring in the subcutaneous tissue of rats after injection of surfactants or carcinogens were compared. Twice weekly subcutaneous injections of the surfactants Blue VRS and Light Green SF elicited a deranged connective tissue repair with continued proliferation of fibroblasts and extensive collagen desposition. In contrast, the carcinogens N-methyl-N-nitrosourea (MNU) and N-nitroquinoline-N-oxide (NQO) appeared to inhibit connective tissue repair and produce morphologically abnormal fibroblasts. The spectrum of neoplastic response was also found to differ. Surfactants gave rise to local sarcomata only after about 47 weeks, whereas carcinogens produced local sarcomata and adenocarcinomata after 20 and 12 weeks respectively.WE have previously reported the results of investigations of the chemical, physical and biological factors involved in the production of local sarcomata in rodents by repeated subcutaneous injection of a variety of chemicals. The relevance of these sarcomata in terms of the potential carcinogenic risk of such chemicals to man have been evaluated Hooson and CGrasso, 1971). These investigations were based on a sequential study of the local tissue reactions to repeated injections and the analysis of the physical properties of compounds involved. A correlation was found between the early lesion induced in the subcutaneous tissues by a shortterm series of 10-20 injections given at the same site and the production of local connective tissue tumouirs by a long-term series of injections. Histologically, it was found possible to distinguish 4 types of reaction (types I IV). Type I and II reactions were mild and self-limiting and did not progress to neoplasia. In contrast, type III and IV reactions were progressive and proliferative and injection site sarcomata were invariably produced subsequently (Grasso and Golberg, 1966a). It was found that compounds eliciting a type III or IV reaction possessed certain physicochemical properties (surface activity, hypertonicity, etc.) capable of pro-(lucing cell injury. Thus it appears that sarcomata which followed the induction of a tvpe III or IV reaction were an indirect result of repeated injury to local fibroblasts and not due to a process of direct chemical carcinogenesis.In contrast, a short-term study of the lesions induced by repeated subcutaneous injection of water soluble carcinogens, whose oncogenic potencies have been demonstrated by other methods of administration, gave different results. The lesion was characterized in all cases by an inhibition of the normal reparative response and the appearance of cytologically abnormal cells . WAe felt that it was important at this stage to extend these observations from the initial 5 weeks and to compare (1) the changes occurring from this time up to the appearance of tumours (the intermediate period)

show abstract

Early Reactions of the Subcutaneous Tissue to Repeated Injections of Carcinogens in Aqueous Solutions

Cited by 15 publications

References 14 publications

Cellular progression of neoplasia in the subcutis of mice after implantation of 3,4-benzpyrene

Cellular progression of neoplasia in the subcutis of mice after implantation of 3,4-benzpyrene

Origins of Injection-Site Sarcomas in Cats: The Possible Role of Chronic Inflammation—A Review

Injection Site Tumours and Preceding Pathological Changes in rats Treated Subcutaneously with Surfactants and Carcinogens

Contact Info

Product

Resources

About