Early reoxygenation in tumors after irradiation: Determining factors and consequences for radiotherapy regimens using daily multiple fractions

Crokart, Nathalie; Jordan, Bénédicte F.; Baudelet, Christine; Ansiaux, Réginald; Sonveaux, Pierre; Grégoire, Vincent; Beghein, Nelson; DeWever, Julie; Bouzin, Caroline; Feron, Olivier; Gallez, Bernard

doi:10.1016/j.ijrobp.2005.02.038

Cited by 84 publications

(78 citation statements)

References 35 publications

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“…Also, the time until a steadystate level of hypoxia was reached after irradiation differed between tumor models (26-29, 31, 49). Although they were not observed in the current study, differences in the effects of radiation on tumor perfusion have been reported (27,28,31,59). Comparison of different studies may be influenced by methodological differences, but tumor line-dependent differences have been reported within the same study (29,37).…”

Section: Clinical Relevancecontrasting

confidence: 52%

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Dynamics of Hypoxia, Proliferation and Apoptosis after Irradiation in a Murine Tumor Model

et al. 2006

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Section: Clinical Relevancecontrasting

confidence: 52%

“…Measurements of pO 2 have shown that early reoxygenation occurs within 3-6 h after irradiation. It was hypothesized that this is caused by both decreased oxygen consumption and increased oxygen delivery (28,31).…”

Section: Introductionmentioning

confidence: 99%

Dynamics of Hypoxia, Proliferation and Apoptosis after Irradiation in a Murine Tumor Model

et al. 2006

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“…4B) (192). This is in agreement with other studies showing that radiation-induced temporary improvements of tumor oxygenation can occur within hours and may last for a few days (151,(199)(200)(201)(202)(203). Therefore, in many tumors, a radiation-induced improvement in oxygenation status will affect the efficacy of the subsequent dose in a fractionated treatment schedule.…”

Section: Dynamics Of Hypoxic Cells After Irradiationsupporting

confidence: 89%

Dynamics of Tumor Hypoxia Measured with Bioreductive Hypoxic Cell Markers

et al. 2007

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Hypoxic cells are common in tumors and contribute to malignant progression, distant metastasis and resistance to radiotherapy. It is well known that tumors are heterogeneous with respect to the levels and duration of hypoxia. Several strategies, including high-oxygen-content gas breathing, radiosensitizers and hypoxic cytotoxins, have been developed to overcome hypoxia-mediated radioresistance. However, with these strategies, an increased tumor control rate is often accompanied by more severe side effects. Consequently, development of assays for prediction of tumor response and early monitoring of treatment responses could reduce both overand undertreatment, thereby avoiding unnecessary side effects. The purpose of this review is to discuss different assays for measurement of hypoxia that can be used to detect changes in oxygen tension. The main focus is on exogenous bioreductive hypoxia markers (2-nitroimidazoles) such as pimonidazole, CCI-103F, EF5 and F-misonidazole. These are specifically reduced and bind to macromolecules in viable hypoxic cells. A number of these bioreductive drugs are approved for clinical use and can be detected with methods ranging from noninvasive PET imaging (low resolution) to microscopic imaging of tumor sections (high resolution). If the latter are stained for multiple markers, hypoxia can be analyzed in relation to different microenvironmental parameters such as vasculature, proliferation and endogenous hypoxia-related markers, for instance HIF1␣ and CA-IX. In addition, temporal and spatial changes in hypoxia can be analyzed by consecutive injection of two different hypoxia markers. Therefore, bioreductive exogenous hypoxia markers are promising as tools for development of predictive assays or as tools for early treatment monitoring and validation of potential endogenous hypoxia markers. ᭧

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“…Other investigators reported that an increase in oxygen partial pressure was observed 3-4 h or 6 h after irradiation by using other murine tumors with EPR oximetry [25,26]. The timing of reoxygenation in RIF-1 murine tumors was determined using EPR oximetry [27].…”

Section: Discussionmentioning

confidence: 99%

Tissue oxygenation in a murine SCC VII tumor after X-ray irradiation as determined by EPR spectroscopy

Fujii¹,

Sakata

Katsumata

et al. 2008

Radiotherapy and Oncology

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Purpose-The goal of this study was to clarify the dynamics of tumor oxygen (partial pressure of oxygen, pO 2 ) in SCC VII murine tumors in mice after X-ray irradiation.Materials and methods-Changes in pO 2 in tumors were measured by 1.2-GHz electron paramagnetic resonance (EPR) spectroscopy after they were exposed to various doses of irradiation. The pO 2 in tumors was followed for up to six days after irradiation at doses of 0, 5, 10, 15, and 20 Gy. Paramagnetic crystals were used as an oximetry probe and implanted into normal or tumor tissues in mice for prolonged periods.Results-The pattern of tumor oxygen after a single dose of radiation with the 5-Gy dose was different from those with other doses (10, 15, and 20 Gy). After 5 Gy, pO 2 increased rapidly (P < 0.01, Student's t test) and then returned to the level observed before irradiation by 12 hours (P < 0.01). In contrast, after 10, 15, or 20 Gy, pO 2 increased rapidly by 6 h after irradiation, continued to increase until at least 24 h (P < 0.01), and then gradually decreased.Conclusion-In tumors that received 5 Gy, post-irradiation increases in pO 2 at 4 h after irradiation were detected by EPR oximetry (P < 0.01) noninvasively.

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Early reoxygenation in tumors after irradiation: Determining factors and consequences for radiotherapy regimens using daily multiple fractions

Cited by 84 publications

References 35 publications

Dynamics of Hypoxia, Proliferation and Apoptosis after Irradiation in a Murine Tumor Model

Dynamics of Hypoxia, Proliferation and Apoptosis after Irradiation in a Murine Tumor Model

Dynamics of Tumor Hypoxia Measured with Bioreductive Hypoxic Cell Markers

Tissue oxygenation in a murine SCC VII tumor after X-ray irradiation as determined by EPR spectroscopy

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