Early‐responsive molecular signatures associated with halophytic adaptation in Sesuvium portulacastrum (L.)

Abstract

Sesuvium portulacastrum (L.) is a halophyte, adapted to grow naturally under saline environments. The ability to use Na and K interchangeably indicated its facultative halophyte nature. No significant growth reduction occurs in seedlings up to 250 mM NaCl, except for curling of the youngest leaf. Within 8 h of salt treatment, seedlings accumulate proline, glycine betaine and other amino acids in both root and shoot. Despite a continued increase of tissue Na content, the number of differentially expressed genes (DEGs) decreases between 8 and 24 h of salt exposure, indicating transcriptional restoration after the initial osmotic challenge. At 8 h, upregulated genes mainly encode transporters and transcription factors, while genes in growth‐related pathways such as photosynthesis and ribosome‐associated biogenesis are suppressed. Overexpression of SpRAB18 (an ABA‐responsive dehydrin), one of the most strongly induced DEGs, in soybean was found to increase biomass in control conditions and the growth benefit was maintained when plants were grown in 100 mM NaCl, indicating conservation of function in halophyte and glycophyte. An open‐access transcriptome database “SesuviumKB” (https://cb.imsc.res.in/sesuviumkb/) was developed to involve the scientific community in wide‐scale functional studies of S. portulacastrum genes, that could pave the way to engineer salt tolerance in crops.

Exploring the potentials of Sesuvium portulacastrum L. for edibility and bioremediation of saline soils

“…Recently, S. portulacastrum has been increasingly studied by physiological ( Slama et al., 2006 ; Peng et al., 2019 ; Wang et al., 2023 ), proteomic ( Cao et al., 2023 ), metabolomic ( Kulkarni et al., 2022 ), and molecular ( Chen Y. et al, 2022 ; Wang et al., 2022 ; Kulkarni et al., 2023 ) analyses of its tolerance to salt stress. This tolerance is not due to the avoidance of roots to Na or the restriction of Na in the roots; rather, it is attributed to the root absorption of Na and its transport from the roots to the shoots ( Wang et al., 2012 ).…”