Early stages of age-related macular degeneration: an immunofluorescence and electron microscopy study.

Abstract

The purpose of this study was to investigate whether immune complex deposits can be detected in maculae with early stages of ARMD and to explain the assumed macrophage reaction before the disciform reaction. We examined a series of human maculae by direct immunofluorescence light microscopy with antibodies against immunoglobulins, fibrinogen, and complement factors. Transmission electron microscopy was performed to identify the macrophages. Materials and methodsThe maculae of 20 human eyes from 13 subjects were obtained at autopsy or after surgical enucleation for intraocular melanoma. The ages 657 on 7 May 2018 by guest. Protected by copyright.

CX3CR1-dependent subretinal microglia cell accumulation is associated with cardinal features of age-related macular degeneration

“…Moreover, CX3CR1 deposits were found in drusen ( Figure 1F). Similar drusen deposits have previously been reported to contain apolipoprotein E, complement factors, MHC, and amyloid oligomers, among others (5,(33)(34)(35)(36). When activated, MCs can express ApoE (37), complement factors (38), MHC (39), and the β-amyloid precursor protein (40) as well as CX3CR1.…”

Avaliação das alterações precoces na coróide e esclera ocorridas em coelhos hipercolesterolêmicos: estudo histológico e histomorfométrico

“…É importante lembrar que processos imunológicos e inflamatórios também são observados na DMRI visto que a análise imuno-histoquímica das drusas revela presença de apolipoproteínas B e E, imunoglobulinas, fator X, componente amiloidal P, complemento C5 e C5b-9, fibrinogênio e vitronectin, assim como exames histológicos da membrana neovascular ou cicatriz disciforme mostram a presença de macrófagos adjacentes às áreas adelgaçadas ou roturas da membrana de Bruch (25)(26)(27)(28) . Estes fatos podem demonstrar que a aterosclerose e DMRI compartilham de mecanismos fisopatogênicos similares.…”

Human Ocular Drusen Possess Novel Core Domains with a Distinct Carbohydrate Composition

“…One impediment encountered in efforts to determine the origin of drusen relates to our overall lack of knowledge about their composition. To date, only a few studies have sought to identify and/or characterize drusen-associated proteins (Newsome et al 1987;van der Schaft et al 1993;Hageman et al 1999;Hageman and Mullins in press), lipids (Pauleikhoff et al 1992;Sheraidah et al 1993), glycosaminoglycans (Kliffen et al 1996), and other carbohydrates (Kliffen et al 1994;Mullins et al 1997). To better understand the composition of different drusen phenotypes, we previously characterized drusen-associated glycoconjugates using lectin histochemistry (Mullins et al, 1997).…”