Early succession of bacterial communities associated as biofilm-like structures in the rhizosphere of alfalfa

Nievas, Fiorela; Primo, Emiliano David; Foresto, Emiliano; Cossovich, Sacha; Giordano, Walter; Bogino, Pablo

doi:10.1016/j.apsoil.2020.103755

Cited by 7 publications

(4 citation statements)

References 61 publications

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“…Under adverse environmental condition, adhesion of beneficial microbial cells like halotolerant K. radicincitans to the plant surface, known as the biofilm, is a critical step for development and fortification of agricultural crops against harmful factors, including salinity. In natural settings, bacteria prefer to live in biofilms rather than planktonic cells (Nievas et al, 2021 ). The root colonization of R. sativus (L.) by KR-17 cells co-cultivated with NaCl, on the other hand, is a mystery.…”

Section: Resultsmentioning

confidence: 99%

PGPR Kosakonia Radicincitans KR-17 Increases the Salt Tolerance of Radish by Regulating Ion-Homeostasis, Photosynthetic Molecules, Redox Potential, and Stressor Metabolites

et al. 2022

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Among abiotic stresses, salinity is a significant limiting factor affecting agricultural productivity, survival, and production, resulting in significant economic losses. Considering the salinity problem, the goal of this study was to identify a halotolerant beneficial soil bacterium to circumvent salinity-induced phytotoxicity. Here, strain KR-17 (having an irregular margin; a mucoid colony; Gm-ve short rod; optimum temperature, 30°C; pH 7.0; no any pigmentation; showed a positive response to citrate utilization, catalase, starch, sucrose, lactose, and dextrose, etc.) recovered from rhizosphere soils of the potato-cultivating field, tolerated surprisingly a high (18% NaCl; 3.-M concentration) level of salt and identified as Kosakonia radicincitans (Accession No. OM348535). This strain was discovered to be metabolically active, synthesized essential PGP bioactive molecules like indole-3-acetic acid (IAA), siderophore (iron-chelating compounds), ACC deaminase, and ammonia, the quantity of which, however, increased with increasing NaCl concentrations. Here, Raphanus sativus L. (radish) was taken as a model crop to evaluate the adverse impact of NaCl, as well as salinity alleviation by halotolerant K. radicincitans. Salinity-induced toxicity to R. sativus was increased in a dose-dependent way, as observed both in vitro and in vivo conditions. Maximum NaCl levels (15%) demonstrated more extreme harm and considerably reduced the plant's biological features. However, membrane damage, relative leaf water content (RLWC), stressor metabolites, and antioxidant enzymes were increased as NaCl concentration increased. In contrast, halotolerant K. radicincitans KR-17 relieved salinity stress and enhanced the overall performance of R. sativus (L.) by increasing germination efficiency, dry biomass, and leaf pigments even in salt-challenged conditions. Additionally, KR-17 inoculation significantly (p ≤ 0.05) improved plant mineral nutrients (Na, K, Ca, Mg, Zn, Fe, Cu, P, and N). Following inoculation, strain KR-17 enhanced the protein, carbohydrates, root pigments, amino acids (AsA and Lys), lipids, and root alkaloids in R. sativus (L.). Besides these, due to PGPR seed priming in NaCl-stressed/non-stressed conditions, membrane damage, RLWC, stressor metabolites, and antioxidant defense enzymes were dramatically reduced. The strong biofilm-forming capacity of K. radicincitans could result in both in vitro and in vivo colonization under NaCl stress. Conclusively, halotolerant K. radicincitans KR-17 may probably be investigated affordably as the greatest way to increase the production of radish under salinity-stressed soils.

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Section: Resultsmentioning

confidence: 99%

PGPR Kosakonia Radicincitans KR-17 Increases the Salt Tolerance of Radish by Regulating Ion-Homeostasis, Photosynthetic Molecules, Redox Potential, and Stressor Metabolites

et al. 2022

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“…As in soil, microbial diversity is lower in the rhizoplane than in the surrounding environment and lower again in the endosphere [19]. Highly adhesive bacteria have an advantage when first colonising living roots, and these strains can initiate a process of succession by facilitating colonisation by competitor strains [44]. This trait is likely to be more important in a hydroponic environment due to the substantial bulk movement of solution through the medium, compared with the normally very low rate of dispersion in soil.…”

Section: Community Ecology In the Hydroponic Rhizobiomementioning

confidence: 99%

The Hydroponic Rockwool Root Microbiome: Under Control or Underutilised?

et al. 2023

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Land plants have an ancient and intimate relationship with microorganisms, which influences the composition of natural ecosystems and the performance of crops. Plants shape the microbiome around their roots by releasing organic nutrients into the soil. Hydroponic horticulture aims to protect crops from damaging soil-borne pathogens by replacing soil with an artificial growing medium, such as rockwool, an inert material made from molten rock spun into fibres. Microorganisms are generally considered a problem to be managed, to keep the glasshouse clean, but the hydroponic root microbiome assembles soon after planting and flourishes with the crop. Hence, microbe–plant interactions play out in an artificial environment that is quite unlike the soil in which they evolved. Plants in a near-ideal environment have little dependency on microbial partners, but our growing appreciation of the role of microbial communities is revealing opportunities to advance practices, especially in agriculture and human health. Hydroponic systems are especially well-suited to active management of the root microbiome because they allow complete control over the root zone environment; however, they receive much less attention than other host–microbiome interactions. Novel techniques for hydroponic horticulture can be identified by extending our understanding of the microbial ecology of this unique environment.

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“…1,2 Biofilms could be defined as complexes of single or multispecies colonies of microorganisms residing in a selfproduced extracellular polymeric substance (EPS) matrix. 1−3 It has been estimated that the EPS contains about 97% of water 2,4 and 3% complex composite of polysaccharides, glycoproteins, lipids, and eDNAs. 1−5 EPS plays an important role not only in supporting the biofilm microstructure but also by controlling nutrients and metabolic waste transports as well as in protecting the residing bacteria from external harsh conditions, such as antibiotics, toxic metals, biocides, UV radiation, immune response molecules, etc.…”

Section: Introductionmentioning

confidence: 99%

“…Bacterial species can resist a range of antibiotics through various mechanisms, which also include the formation of biofilms. , Biofilms could be defined as complexes of single or multispecies colonies of microorganisms residing in a self-produced extracellular polymeric substance (EPS) matrix. − It has been estimated that the EPS contains about 97% of water , and 3% complex composite of polysaccharides, glycoproteins, lipids, and eDNAs. − EPS plays an important role not only in supporting the biofilm microstructure but also by controlling nutrients and metabolic waste transports as well as in protecting the residing bacteria from external harsh conditions, such as antibiotics, toxic metals, biocides, UV radiation, immune response molecules, etc. − The EPS matrix can impede the penetration of antibiotics from reaching the embedded bacterial cells in the biofilm. ,, This occurs through absorption, deactivation, neutralization, and degradation of antibiotics by pH and extracellularly secreted enzymes in the biofilm microenvironment, rendering the bacteria in the biofilm more resistant than planktonic cells of the same strain. , …”

Section: Introductionmentioning

confidence: 99%

Targeting S. epidermidis Biofilms by the Tetracycline-Loaded Nanogel Surface Functionalized with Savinase, DNase, and Cellulase

Asare,

Seidakhanova,

Amangeldinova

et al. 2023

ACS Appl. Nano Mater.

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Early succession of bacterial communities associated as biofilm-like structures in the rhizosphere of alfalfa

Cited by 7 publications

References 61 publications

PGPR Kosakonia Radicincitans KR-17 Increases the Salt Tolerance of Radish by Regulating Ion-Homeostasis, Photosynthetic Molecules, Redox Potential, and Stressor Metabolites

PGPR Kosakonia Radicincitans KR-17 Increases the Salt Tolerance of Radish by Regulating Ion-Homeostasis, Photosynthetic Molecules, Redox Potential, and Stressor Metabolites

The Hydroponic Rockwool Root Microbiome: Under Control or Underutilised?

Targeting S. epidermidis Biofilms by the Tetracycline-Loaded Nanogel Surface Functionalized with Savinase, DNase, and Cellulase

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