In recent years, the dry-wet transition (DWT), which often leads to regional floods and droughts, has become increasingly frequent in the Poyang Lake basin and the Dongting Lake basin (hereinafter referred to as the two-lake region). This study aims to investigate the early warning signals (EWSs) for DWT events. Firstly, based on the standardized precipitation index (SPI) at 161 meteorological stations in the two-lake region from 1961 to 2020, the two-lake region is divided into four sub-regions by the Rotational Empirical Orthogonal Function (REOF) analysis method. Then, the occurrence time of the DWT events in each sub-region is determined by the moving t-test (MTT) technique. Finally, by using two indicators (variance and the auto-correlation coefficient) to describe the critical slowing down (CSD) phenomenon, the EWSs denoting the DWT events in all sub-regions are investigated. The results reveal that there was a significant dry-to-wet (wet-to-dry) event around 1993 (2003) in the two-lake region during the last 60 years. The phenomenon of CSD, where the auto-correlation coefficient and variance increases are found in all sub-regions around 10 years before the DWT, suggests that it can be taken as an EWS for the DWT events. This study confirms the effectiveness of applying the slowing down theory in investigating the EWSs for abrupt changes in the two-lake region, aiming to provide a theoretical basis for effective prevention and mitigation against disasters in this region. Moreover, it is expected to be well-applied to the middle and lower reaches of the Yangtze River.