Earthworm-assisted bioremediation of petroleum hydrocarbon contaminated soil from mechanic workshop

Ameh, AO; Mohammed-Dabo, I. A.; Ibrahim, Sani; Ameh, JB

doi:10.5897/ajest2013.1506

Cited by 12 publications

(11 citation statements)

References 21 publications

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“…They explained that loss in organic carbon was due to the use of organic carbon by earthworms and microorganisms as source of energy. Similar trend was also reported by Kennette et al (2002) with Lumbricus terrestris , Ceccanti et al (2006) with E. fetida and Ameh et al (2011) with L. terrestris , while Singer et al (2001), Schaefer et al (2005), Iordache and Borza (2012) and Ameh et al (2013) reported higher values.…”

Section: Discussionsupporting

confidence: 83%

“…Results showed that the earthworms could remove nearly 80 % of the PAHs as compared to just 47 and 21 % where it was not used and only microbial degradation occurred. Ameh et al (2013) investigated the use of earthworms ( Eudrilus eugeniae ) for vermi-assisted bioremediation of petroleum hydrocarbon-contaminated mechanic workshop soils. After 35 days of treatment, earthworm inoculation affected a higher drop in total petroleum hydrocarbon contents as compared to the samples without worms, indicating that earthworms may be used as biocatalysts in the bioremediation process.…”

Section: Introductionmentioning

confidence: 99%

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Bioremediation of petroleum hydrocarbons from crude oil-contaminated soil with the earthworm: Hyperiodrilus africanus

Ekperusi

Aigbodion

2015

3 Biotech

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A study on the bioremediation potentials of the earthworm Hyperiodrilusafricanus (Beddard) in soil contaminated with crude oil was investigated. Dried and sieved soils were contaminated with 5 ml each of crude oil with replicates and inoculated with earthworms and monitored daily for 12 weeks. Physicochemical parameters such as pH, total organic carbon, sulfate, nitrate, phosphate, sodium, potassium, calcium and magnesium were determined using standard procedures. Total petroleum hydrocarbon (TPH) was determined using atomic absorption spectrophotometer (AAS), while BTEX constituents and earthworms tissues were analyzed using Gas Chromatography with Flame Ionization Detector (GC-FID). The results showed that the earthworm significantly enhanced the physicochemical parameters of the contaminated soil resulting in a decrease of the total organic carbon (56.64 %), sulfate (57.66 %), nitrate (57.69 %), phosphate (57.73 %), sodium (57.69 %), potassium (57.68 %), calcium (57.69 %) and magnesium (57.68 %) except pH (3.90 %) that slightly increased. There was a significant decrease in the TPH (84.99 %), benzene (91.65 %), toluene (100.00 %), ethylbenzene (100.00 %) and xylene (100.00 %). Analyses of the tissues of the earthworm at the end of the experiment showed that the earthworms bioaccumulated/biodegraded 57.35/27.64 % TPH, 38.91/52.73 % benzene, 27.76/72.24 % toluene, 42.16/57.85 % ethylbenzene and 09.62/90.38 % xylene. The results showed that the earthworms H. africanus could be used to bioremediate moderately polluted soil with crude oil contamination in the Niger Delta region of Nigeria.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Bioremediation of petroleum hydrocarbons from crude oil-contaminated soil with the earthworm: Hyperiodrilus africanus

Ekperusi

Aigbodion

2015

3 Biotech

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“…hydrocarbon utilizers and their proportions within the microbial community appear to be a sensitive index of environmental exposure to hydrocarbons [13]. It has also become a universally acceptable technology for the removal of a wide range of contaminants, especially generated from the petrochemical industries from the environment as it is a relatively cheap and effective method of remediation compared to other methods [14].…”

Section: Issn: 2455-0299mentioning

confidence: 99%

“…Ameh et al [14] investigated the use of earthworms (Eudrilus eugeniae) for vermi-assisted bioremediation of petroleum hydrocarboncontaminated mechanic workshop soils. After 35 days of treatment, earthworm inoculation affected a higher drop in total petroleum hydrocarbon contents as compared to the samples without worms, indicating that earthworms may be used as biocatalysts in the bioremediation process.…”

Section: Issn: 2455-0299mentioning

confidence: 99%

Enumeration and Identification of Microbial Load in Oil Spilled Soil Remediated using Eudrilus eugeniae

Asubiaro¹,

Njoku²,

Amechina³

et al. 2018

JNPR

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The scope of this study was to enumerate and identify microorganisms present in crude oil polluted soil before and after remediation with Eudrilus eugeniae using conventional method for enumeration and molecular tools (ITS 1 and ITS 4 primers) for identification. A crude oil polluted soil from Khana Local Government of Rivers State Nigeria and also soil from botanical garden of the University of Lagos were used. These soil samples were measured in four different concentrations (25%, 50%, 75% and 100%). Each of these concentrations was experimental setup of addition of substrate alone, E. eugeniae and substrate and E. eugeniae alone. These setups were made in triplicate amounting to a total of thirty six experimental setups. Enumeration of microbial load was done before and after addition of each of the content of the experimental setups using conventional method and then identification of microbes was done using molecular tools. The result shows total heterotrophic fungi was 2.9 x 10 4 cfu/m/g, while the total heterotrophic bacteria was 4.4 x 10 5 cfu/m/g before remediation. After remediation, total heterotrophic fungi was 1.6 x 10 5 cfu/m/g while total heterotrophic bacteria was 5.0 x 10 4 cfu/m/g. This shows that microorganisms were present in the soil before and after remediation. The increase in number of microbes suggests a favorable condition for continuity of microbial life. Fungi such as Trichoderma asperellum, Aspergillus flavus, Penicillum chrysogenum and another strain of Aspergillus flavus were identified. The bacteria sequencing failed suspected to be due to primers used or error with sequencing plates. In conclusion, the results of this study confirmed the presence of microorganisms in crude oil polluted soils. The enumeration and identification of the bacteria and fungi in this experiment will help to enhance in situ clean-up of crude oil polluted soil.

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“…The residue, (soil), was allowed to dry in an oven at 50°C. The TPH was calculated according to [1]; [2]: Table 3 showed the values for the soil parameters that were tested. …”

Section: Total Petroleum Hydrocarbon Analysismentioning

confidence: 99%

Optimisation of Diesel Polluted Soil Using Response Surface Methodology

Olawale

Oyawale²,

Adepoju³

et al. 2015

IJEPP

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Abstract:This research is based on the investigation of the bioremediation of diesel polluted soil using biostimulation strategy. The study was carried out on a diesel contaminated soil and concentrations: Tween 80 (5-15ml), Poultry droppings (20-60g), Hydrogen Peroxide (0.5-1.5ml) were added and incubated for 28 days of remediation period. Response Surface Methodology with Box-Behnken Design (BBD) was used with three factors and three levels of Tween 80, Poultry droppings and Hydrogen Peroxide as independent variables while diesel oil (Total Petroleum Hydrocarbon) removal was the dependent variable (response). The result showed disparities in the diesel oil biodegradation pattern with respect to Tween 80, Poultry droppings and Hydrogen Peroxide. The statistical analysis, via ANOVA showed coefficient of determination R 2 to be 99.89% and P < 0.05. The predicted optimum parameter of Tween 80: 10.10ml, Poultry droppings: 41.46g and Hydrogen Peroxide: 1.10ml were gotten while 56.565% was gotten as the optimal diesel oil removal. At this optimum condition, the observed diesel oil removal was found to be 56.568%. It can be concluded that bioremediation of diesel-contaminated soil resulted in petroleum hydrocarbon degradation.

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Earthworm-assisted bioremediation of petroleum hydrocarbon contaminated soil from mechanic workshop

Cited by 12 publications

References 21 publications

Bioremediation of petroleum hydrocarbons from crude oil-contaminated soil with the earthworm: Hyperiodrilus africanus

Bioremediation of petroleum hydrocarbons from crude oil-contaminated soil with the earthworm: Hyperiodrilus africanus

Enumeration and Identification of Microbial Load in Oil Spilled Soil Remediated using Eudrilus eugeniae

Optimisation of Diesel Polluted Soil Using Response Surface Methodology

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