Easy Fabrication of Thin Membranes with Through Holes. Application to Protein Patterning

Masters, Thomas A.; Engl, Wilfried; Weng, Zhe; Arasi, Bakya; Gauthier, Nils C.; Viasnoff, Virgile

doi:10.1371/journal.pone.0044261

Cited by 39 publications

(32 citation statements)

References 35 publications

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“…To investigate this further, we examined frustrated phagocytosis on 30-μm circles of IgG (18). Cells that landed on the circle edge extended pseudopods isotropically over both coated and noncoated areas alike, up to the transition ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis

Masters

Pontes

Viasnoff

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Phagocytes clear the body of undesirable particles such as infectious agents and debris. To extend pseudopods over the surface of targeted particles during engulfment, cells must change shape through extensive membrane and cytoskeleton remodeling. We observed that pseudopod extension occurred in two phases. In the first phase, pseudopods extended rapidly, with actin polymerization pushing the plasma membrane forward. The second phase occurred once the membrane area from preexisting reservoirs was depleted, leading to increased membrane tension. Increased tension directly altered the small Rho GTPase Rac1, 3′-phosphoinositide, and cytoskeletal organization. Furthermore, it activated exocytosis of vesicles containing GPI-anchored proteins, increasing membrane area and phagocytosis efficiency for large particles. We thus propose that, during phagocytosis, membrane remodeling, cytoskeletal organization, and biochemical signaling are orchestrated by the mechanical signal of membrane tension. These results put a simple mechanical signal at the heart of understanding immunological responses.

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Section: Resultsmentioning

confidence: 99%

Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis

Masters

Pontes

Viasnoff

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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189

204

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“…Cells were seeded on substrates containing circular adhesive islands of varying area (500, 750, 1000, 1500, 2000 and 2500µm 2 ), or, circular islands with fixed areas (700, 1200 or 1800 µm 2 ). Adhesive circular islands were fabricated by using a PDMS stamp in either micro-contact printing as described previously 15 , or, by a slightly modified version of stencil patterning 44 . In stencil patterning, PDMS stamps were first inverted and placed onto ibidi hydrophobic uncoated 35mm µ-dishes (ibidi GmbH).…”

Section: Protein Micropatterning Of Substratesmentioning

confidence: 99%

Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement

Jalal

Huang

Viasnoff

et al. 2018

Preprint

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We systematically investigated the principles of actin cytoskeleton self-organization in two cell types, fibroblasts and epitheliocytes, by confining isolated cells on isotropic adhesive islands of varying size. In fibroblasts, we previously described that an initially circular pattern of circumferential actin dynamically evolves into a radial pattern of actin bundles that spontaneously transforms into a chiral pattern, before finally producing parallel linear stress fibres. We now show that progression from circular to chiral actin patterns depends on cell projected area and rarely occurs on small islands. Epitheliocytes however, did not exhibit succession through all the actin patterns described above even on large islands. Upon confinement, the actin cytoskeleton in non-keratinocyte epitheliocytes is arrested at the circular stage, while in keratinocytes it can progress as far as the radial pattern but still cannot break symmetry. Epithelial-mesenchymal transition pushed actin cytoskeleton development from circular towards radial patterns but remains insufficient to cause chirality. Surprisingly, small doses of G-actin sequestering drug, latrunculin A, induced chiral swirling in keratinocytes. During this swirling, keratin filaments follow actin and also demonstrate chiral swirling movement. Elimination of the keratin network by genetic silencing of Type II keratins, however, did not affect the self-organization of the actin cytoskeleton.

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“…Many techniques can be used to lay down a pattern, with most being micron-scale approaches usually used in the textile industry. These include micro-serigraphy or micro-stenciling, which uses removable membrane with holes of different shapes to mask the exposed area of the surface (Masters et al, 2012;Ostuni et al, 2000), and micro-stamping, which uses soft textured material to 'ink' the region of contact with proteins (Piel and Thery, 2014b). Another approach is dip-pen lithography, which uses deposition of protein with a sharp tip (Salaita et al, 2007).…”

Section: Brief Overview Over Bio-functionalized Substratesmentioning

confidence: 99%

How cells respond to environmental cues – insights from bio-functionalized substrates

Ruprecht

Monzo

Ravasio

et al. 2016

Journal of Cell Science

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Biomimetic materials have long been the (he)art of bioengineering. They usually aim at mimicking in vivo conditions to allow in vitro culture, differentiation and expansion of cells. The past decade has witnessed a considerable amount of progress in soft lithography, bioinspired micro-fabrication and biochemistry, allowing the design of sophisticated and physiologically relevant micro-and nanoenvironments. These systems now provide an exquisite toolbox with which we can control a large set of physicochemical environmental parameters that determine cell behavior. Biofunctionalized surfaces have evolved from simple protein-coated solid surfaces or cellular extracts into nano-textured 3D surfaces with controlled rheological and topographical properties. The mechanobiological molecular processes by which cells interact and sense their environment can now be unambiguously understood down to the single-molecule level. This Commentary highlights recent successful examples where bio-functionalized substrates have contributed in raising and answering new questions in the area of extracellular matrix sensing by cells, cell-cell adhesion and cell migration. The use, the availability, the impact and the challenges of such approaches in the field of biology are discussed.

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Easy Fabrication of Thin Membranes with Through Holes. Application to Protein Patterning

Cited by 39 publications

References 35 publications

Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis

Plasma membrane tension orchestrates membrane trafficking, cytoskeletal remodeling, and biochemical signaling during phagocytosis

Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement

How cells respond to environmental cues – insights from bio-functionalized substrates

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