Easy Synthesis of Doped Graphitic Carbon Nitride Nanosheets as New Material for Enhanced DNA Extraction from Vegetal Tissues Using a Simple and Fast Protocol

Abstract

Conventional and commercially available DNA extraction methods have several limitations regarding, for instance, contamination, and complex and slow precipitation and recovery processes. Herein, we report the synthesis of oxygen and phosphorus-doped Graphitic carbon nitride structures (g-POCN), via a novel Zinc-catalyzed one-pot solvothermal approach, and its application in the extraction of genomic DNA (gDNA) from a vegetal matrix (P. argentatum). Experimental and molecular modeling analyses demonstrate the high affinity of gDNA with g-POCN, which provided highly efficient gDNA extraction processes, with extraction yield, as well as integrity and quality of the extracted gDNA, comparable or superior to a commercial extraction kit and isopropanol extraction. Moreover, under suitable elution conditions, this method allows the easy removal of high concentrations of gDNA from g-POCN, rendering this method as a low-cost, simple, and fast approach for the extraction of even small amounts of gDNA. Remarkably, the extracted gDNA shows no degradation, and no inhibition of the polymerase chain reaction. Therefore, g-POCN represents a promising material for the highly efficient, cost-effective, and biocompatible extraction of DNA, which could stimulate research focused on broad DNA sources, e.g., RNA extraction, plasmids, ssDNA, etc.

3D Variable Range Hopping Electrical Conduction of a Carbon from Polyaniline Vapor

“…Carbon materials have been paid much attention from the viewpoints of basic research and industrial applications. Many promising new forms of carbon have been found, such as fullerenes, carbon nanotubes, graphene, and carbon composites [1][2][3][4][5][6][7][8][9][10][11][12]. Active carbons have been employed as cleaners, battery electrodes, and capacitors.…”