Ebola virus disease outbreak in the Democratic Republic of the Congo: A mini-review

Bisimwa, Paterne; Biamba, Chrispin; Aborode, Abdullahi Tunde; Cakwira, Hugues; Akilimali, Aymar

doi:10.1016/j.amsu.2022.104213

Cited by 11 publications

(13 citation statements)

References 5 publications

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“…Another scenario is an outbreak in resource-limited settings, where a lack of early diagnosis could result in failure to contain further spread. Indeed, outbreaks often emerge or have the potential to emerge from developing or remote areas. − Moreover, pathogens known or expected to cause outbreaks can often emerge from a zoonotic source especially from wildlife in rural or remote locations. − These include major high-impact pathogens such as Ebola virus, SARS-CoV-2 and its related family SARS-CoV (severe acute respiratory syndrome coronavirus) and MERS-CoV (Middle East respiratory syndrome coronavirus), and Zika virus. − Proactive, on-site SHERLOCK testing in reservoir and intermediate animal hosts could be utilized to greatly facilitate surveillance and research activities contributing to public health preparedness for potential future outbreaks. In addition, SHERLOCK has other advantages (Introduction S1).…”

Section: Introductionmentioning

confidence: 99%

Strategies to Improve Multi-enzyme Compatibility and Coordination in One-Pot SHERLOCK

Kielich

Liu

et al. 2023

Anal. Chem.

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While molecular diagnostics generally require heating elements that supply high temperatures such as 95 °C in polymerase chain reaction and 60–69 °C in loop-mediated isothermal amplification, the recently developed CRISPR-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can operate at 37 °C or a similar ambient temperature. This unique advantage may be translated into highly energy-efficient or equipment-free molecular diagnostic systems with unrestricted deployability. SHERLOCK is characterized by ultra-high sensitivity when performed in a traditional two-step format. For RNA sensing, the first step combines reverse transcription with recombinase polymerase amplification, while the second step consists of T7 transcription and CRISPR-Cas13a detection. The sensitivity drops dramatically, however, when all these components are combined into a single reaction mixture, and it largely remains an unmet need in the field to establish a high-performance one-pot SHERLOCK assay. An underlying challenge, conceivably, is the extremely complex nature of a one-pot formulation, crowding a large number of reaction types using at least eight enzymes/proteins. Although previous work has made substantial improvements by serving individual enzymes/reactions with accommodating conditions, we reason that the interactions among different enzymatic reactions could be another layer of complicating factors. In this study, we seek optimization strategies by which inter-enzymatic interference may be eliminated or reduced and cooperation created or enhanced. Several such strategies are identified for SARS-CoV-2 detection, each leading to a significantly improved reaction profile with faster and stronger signal amplification. Designed based on common molecular biology principles, these strategies are expected to be customizable and generalizable with various buffer conditions or pathogen types, thus holding broad applicability for integration into future development of one-pot diagnostics in the form of a highly coordinated multi-enzyme reaction system.

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Section: Introductionmentioning

confidence: 99%

Strategies to Improve Multi-enzyme Compatibility and Coordination in One-Pot SHERLOCK

Kielich

Liu

et al. 2023

Anal. Chem.

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“…Since 1994, a virus wave has been observed in Africa every 1.5 years. Some researchers believe that the rapid spread of the Ebola virus in 1976 occurred partly due to the vaccination program, virus contaminated sanitary items and contact in different ways, such as sharing meals, conversation, touch or while sharing bed 3 . The Ebola virus has affected Sudan, DRC, the USA, UK, Philippines, Italy etc., with a high magnitude of fatality rate in West and Equatorial African countries 4 .…”

Section: Introductionmentioning

confidence: 99%

Resurgence of Ebola Virus: Transmission, Pathogenesis, Prevention and Cure

Aljowaie¹

2023

PJMHS

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Ebola virus (EBOV) disease is a zoonotic disease that is caused by four species of genus Ebolavirus. The EBOV disease has outbreaks (1976-2022) in West and Central African countries, high mortality rate has made it a public health concern. EBOV is a filamentous virus having negative stranded and non-segmented RNA. The EBOV genome has the unique capability of forming soluble glycoprotein. Since its first emergence in human history, several effective therapeutics and vaccines are developed. These vaccines include replicative or non-replicative vectored vaccines, DNA vaccines and monovalent or polyepitopic vaccines. This article reviewed the structure of EBOV and pathophysiology as well as the immune response of EBOV infection. In addition to diagnosis and vaccinations available to cure EBOV at acute and chronic stages. Keywords: Zoonotic disease, Filovirus, Soluble glycoprotein, DNA vaccines, Polyepitopic, Vectored vaccines, Pathophysiology, Immune response

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“…We realized that the healthcare system of DR Congo being fragile, poor, lacks major sophisticated medical materials, and equipment for surveillance, control, diagnosis, and management of certain infectious diseases, for example, TB, Ebola 2,3 . As the case maybe, TB, being a deadly infectious disease like COVID-19, Meningitis, Ebola, Bubonic, have been shown to kill millions of people in the world including the DR Congo 3,4 . This is a public health challenge in the DR Congo that needs serious attention.…”

mentioning

confidence: 99%

“…The Democratic Republic of Congo (DR Congo), as an African country, has been classified as one of the poorest countries in the world with a weakened and paralyzed healthcare system with various outbreaks of infectious diseases such as tuberculosis (TB) and Corona Virus Disease 2019 (COVID-19) 1 . We realized that the healthcare system of DR Congo being fragile, poor, lacks major sophisticated medical materials, and equipment for surveillance, control, diagnosis, and management of certain infectious diseases, for example, TB, Ebola 2,3 . As the case maybe, TB, being a deadly infectious disease like COVID-19, Meningitis, Ebola, Bubonic, have been shown to kill millions of people in the world including the DR Congo 3,4 .…”

mentioning

confidence: 99%

Challenges and perspective in the fight against tuberculosis in the Democratic Republic of Congo

Akilimali,

Cakwira,

Biamba

et al. 2024

International Journal of Surgery: Global Health

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Ebola virus disease outbreak in the Democratic Republic of the Congo: A mini-review

Cited by 11 publications

References 5 publications

Strategies to Improve Multi-enzyme Compatibility and Coordination in One-Pot SHERLOCK

Strategies to Improve Multi-enzyme Compatibility and Coordination in One-Pot SHERLOCK

Resurgence of Ebola Virus: Transmission, Pathogenesis, Prevention and Cure

Challenges and perspective in the fight against tuberculosis in the Democratic Republic of Congo

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