Ebola virus mediated infectivity is restricted in canine and feline cells

Han, Ziying; Bart, Stephen M.; Ruthel, Gordon; Burgt, Nathan H. Vande; Haines, Kathleen M.; Volk, Susan W.; Vite, Charles H.; Freedman, Bruce D.; Bates, Paul; Harty, Ronald N.

doi:10.1016/j.vetmic.2015.11.011

Cited by 7 publications

(2 citation statements)

References 16 publications

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“…Some evidence suggests that dogs may be infected with EBOV, however remain asymptomatic [10]. While dogs may be susceptible to infection, EBOV replication has been shown to be restricted in canine and feline cells [11], further explaining the apparent asymptomatic display of canines. This suggests that dogs are not likely to be reservoirs but may serve as sentinels as they may mount an immune response when exposed to EBOV.…”

Section: Introductionmentioning

confidence: 99%

Serological evidence of Ebola virus exposure in dogs from affected communities in Liberia: A preliminary report

et al. 2019

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Filoviruses such as Ebola virus (EBOV) cause outbreaks of viral hemorrhagic fevers for which no FDA-approved vaccines or drugs are available. The 2014–2016 EBOV outbreak in West Africa infected approximately 30,000 people, killing more than 11,000 and affecting thousands more in areas still suffering from the effects of civil wars. Sierra Leone and Liberia reported EBOV cases in every county demonstrating the efficient spread of this highly contagious virus in the well-connected societies of West Africa. In communities, canines are often in contact with people while scavenging for food, which may include sickly bush animals or, as reported from the outbreak, EBOV infected human bodies and excrement. Therefore, dogs may serve as sentinel animals for seroprevalence studies of emerging infectious viruses. Further, due to their proximity to humans, they may have important One Health implications while offering specimens, which may be easier to obtain than human serum samples. Previous reports on detecting EBOV exposure in canines have been limited. Herein we describe a pilot project to detect IgG-responses directed against multiple filovirus and Lassa virus (LASV) antigens in dogs from EBOV affected communities in Liberia. We used a multiplex Luminex-based microsphere immunoassay (MIA) to detect dog IgG binding to recombinant filovirus antigens or LASV glycoprotein (GP) in serum from dogs that were old enough to be present during the EBOV outbreak. We identified 47 (73%) of 64 dog serum samples as potentially exposed to filoviruses and up to 100% of the dogs from some communities were found to have elevated levels of EBOV antigen-binding IgG titers. The multiplex MIA described in this study provides evidence for EBOV IgG antibodies present in dogs potentially exposed to the virus during the 2014–16 outbreak in Liberia. These data support the feasibility of canines as EBOV sentinels and provides evidence that seroprevalence studies in dogs can be conducted using suitable assays even under challenging field conditions. Further studies are warranted to collect data and to define the role canines may play in transmission or detection of emerging infectious diseases.

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Section: Introductionmentioning

confidence: 99%

Serological evidence of Ebola virus exposure in dogs from affected communities in Liberia: A preliminary report

et al. 2019

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“…FLNa-specific siRNA, FLNb-specific siRNA and control siRNA pools were purchased from Santa Cruz and Origene. The rVSV-eGP-mCherry virus [ 53 ] was kindly provided by P. Bates (UPenn School of Medicine).…”

Section: Methodsmentioning

confidence: 99%

Contrasting effects of filamin A and B proteins in modulating filovirus entry

Shepley-McTaggart,

Liang,

Ding

et al. 2023

PLoS Pathog

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Ebola (EBOV) and Marburg viruses (MARV) cause severe hemorrhagic fever associated with high mortality rates in humans. A better understanding of filovirus-host interactions that regulate the EBOV and MARV lifecycles can provide biological and mechanistic insight critical for therapeutic development. EBOV glycoprotein (eGP) and MARV glycoprotein (mGP) mediate entry into host cells primarily by actin-dependent macropinocytosis. Here, we identified actin-binding cytoskeletal crosslinking proteins filamin A (FLNa) and B (FLNb) as important regulators of both EBOV and MARV entry. We found that entry of pseudotype psVSV-RFP-eGP, infectious recombinant rVSV-eGP-mCherry, and live authentic EBOV and MARV was inhibited in filamin A knockdown (FLNaKD) cells, but was surprisingly enhanced in filamin B knockdown (FLNbKD) cells. Mechanistically, our findings suggest that differential regulation of macropinocytosis by FLNa and FLNb likely contributes to their specific effects on EBOV and MARV entry. This study is the first to identify the filamin family of proteins as regulators of EBOV and MARV entry. These findings may provide insight into the development of new countermeasures to prevent EBOV and MARV infections.

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Ebola Virus

Kidd¹

2020

Clinical Small Animal Internal Medicine

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Ebola virus mediated infectivity is restricted in canine and feline cells

Cited by 7 publications

References 16 publications

Serological evidence of Ebola virus exposure in dogs from affected communities in Liberia: A preliminary report

Serological evidence of Ebola virus exposure in dogs from affected communities in Liberia: A preliminary report

Contrasting effects of filamin A and B proteins in modulating filovirus entry

Ebola Virus

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