Ecabet sodium alleviates neomycin-induced hair cell damage

Rah, Yoon Chan; Choi, June; Yoo, Myung Hoon; Yum, Gunhwee; Park, Saemi; Oh, Kyoung Ho; Lee, Jun Young; Kwon, Soon Young; Cho, Seung-Hyun; Kim, Suhyun; Park, Hae Chul

doi:10.1016/j.freeradbiomed.2015.11.007

Cited by 12 publications

(9 citation statements)

References 45 publications

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“…The mechanisms underlying the effects on the small intestine are unclear. Recently, in a study using a zebrafish experimental animal model, ES alleviated neomycin-induced hair cell damage through reactive oxygen species scavenging or anti-apoptotic effects [27]. ES might prevent LDA-induced small intestinal mucosal injury through similar mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Preventive effect of ecabet sodium on low-dose aspirin-induced small intestinal mucosal injury: a randomized, double-blind, pilot study

Takeuchi

Kojima

et al. 2019

BMC Gastroenterol

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BackgroundWe aimed to investigate how high-dose ecabet sodium affects low-dose aspirin-induced small intestinal mucosal injury in healthy volunteers.MethodsHealthy volunteers were enrolled randomly into one of two groups with the following drug regimens for 2 weeks: group A, low-dose aspirin once per day and group B, low-dose aspirin and 4.0 g of ecabet sodium. Small bowel capsule endoscopy was performed before and 2 weeks after low-dose aspirin administration.ResultsA significant difference was found in the median number [range] of small intestinal lesions between baseline and two weeks after low-dose aspirin administration in group A (baseline: 1 [0–5], after: 5 [1–11]; p = 0.0059) but not in group B (baseline: 0.5 [0–9], after: 3 [0–23]; p = 0.0586). In group B, although the median number [range] of lesions in the first tertile of the small intestine did not increase two weeks after low-dose aspirin administration (baseline: 0 [0–4], after: 1.5 [0–8]; p = 0.2969), the number of lesions in the second and third tertiles of the small intestine increased significantly (baseline: 0 [0–5], after: 2 [0–15]; p = 0.0469).ConclusionsEcabet sodium had a preventive effect on low-dose aspirin-induced small intestinal mucosal injury in the upper part of the small intestine.Trial registrationISRCTN 99322160, 01/10/2018.

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Section: Discussionmentioning

confidence: 99%

Preventive effect of ecabet sodium on low-dose aspirin-induced small intestinal mucosal injury: a randomized, double-blind, pilot study

Takeuchi

Kojima

et al. 2019

BMC Gastroenterol

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“…Wild-type (AB) and transgenic (Tg; brn3c:EGFP and cmlc2: EGFP) zebrafish were sustained at 28.5℃ under a 14 hours light and 10 hours dark cycle in a zebrafish facility at Korea University Ansan Hospital as previously described [ 7 , 19 ]. Newly hatched brine shrimp ( Artemia ; San Francisco Bay Brand Inc., Newark, CA, USA) were supplied two times per day for the adult fish.…”

Section: Methodsmentioning

confidence: 99%

“…The wild-type and Tg (brn3c:EGFP) larvae were revealed to nicotine at 5, 10, 20, and 40 μM for 120 hours. At 120 hpf, the larvae were rinsed with embryo medium three times and anesthetized with a tricaine (3-aminobenzoic acid 0.4 g/ethyl ester; 100 mL; pH 7, adjusted using Tris buffer) for 5 minutes as previously described [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, apoptosis of hair cell was identified at 120 hpf in Tg (brn3c:EGFP) zebrafish by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining with an in situ cell detection kit (Roche Molecular Biochemicals, Mannheim, Germany) in accordance with the manufacturer’s protocol. TUNEL-positive cells were analyzed in four neuromasts in 10 fish from each group (total n=50), as previously described [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

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Impact of Nicotine Exposure on Hair Cell Toxicity and Embryotoxicity During Zebrafish Development

Yoo

Rah

Park

et al. 2018

Clin Exp Otorhinolaryngol

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Objectives Nicotine has various adverse effects including negative impacts associated with maternal exposure. In the current study, we examined nicotine-induced damage of hair cells and embryotoxicity during zebrafish development.Methods Zebrafish embryos were exposed to nicotine at several concentrations (5, 10, 20, and 40 μM) and embryotoxicity were evaluated at 72 hours, including hatching rate, mortality, teratogenicity rate, and heart rate. Hair cells within the supraorbital (SO1 and SO2), otic (O1), and occipital (OC1) neuromasts were identified at 120 hours. Apoptosis and mitochondrial damage of hair cells were analyzed using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling) and DASPEI (2-[4-(dimethylamino)styryl]-N-ethylpyridinium iodide) assays, respectively, and changes of ultrastructure were observed by scanning electron microscopy.Results The control group without nicotine appeared normal with overall mortality and teratogenicity rate <5%. The hatching rate and mortality rate was not significantly different according to nicotine concentration (n=400 each). The abnormal morphology rate (n=400) increased and heart rate (n=150) decreased with increasing nicotine concentration (P<0.05). Nicotine-induced hair cell damage significantly increased as nicotine concentration increased. A significantly greater number of TUNEL-positive cells (P<0.01) and markedly smaller DASPEI area (P<0.01) were shown as nicotine concentration increased.Conclusion The current results suggest that nicotine induces dose-dependent hair cell toxicity in embryos by promoting apoptosis and mitochondrial and structural damage.

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“…The larvae were washed thrice with embryo medium after treatment and anesthetized using tricaine (3-aminobenzoic acid ethyl ester, 0.4 g/100 mL; pH adjusted to 7.0 using Tris buffer) for 5 minutes as described in previous studies. 16,17 They were then mounted on a depression slide using methylcellulose and observed under a fluorescence microscope (LSM5 PASCAL, Carl Zeiss, Germany) at 40Â magnification.…”

Section: Chemical Treatmentsmentioning

confidence: 99%

Evaluation of Ototoxicity of an Antifog Agent and the Suspected Underlying Mechanisms: An Animal Study

et al. 2019

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Use of rigid endoscopes has become widespread in middle ear surgeries, thereby attracting attention to the safety of antifog agents. However, few studies on the ototoxicity of antifog agents have been conducted. The purpose of this study was to evaluate hair cell damage and the underlying mechanisms caused by antifog agents using zebrafish larvae. We exposed zebrafish larvae at 3 days postfertilization to various concentrations of the antifog agent, Ultrastop (0.01, 0.02, 0.04, and 0.08%) for 72 hours. The average number of hair cells within 4 neuromasts of larvae, including supraorbital (SO1 and SO2), otic (O1), and occipital (OC1), in the control group were compared to those in the exposure groups. Significant hair cell loss was observed in the experimental groups compared to that in the control group (P < .01; control: 53.88 + 4.85, 0.01%: 45.08 + 11.70, 0.02%: 41.36 + 12.00, 0.04%: 35.36 + 16.18, and 0.08%: 15.60 + 7.53 cells). Concentration-dependent increase in hair cell apoptosis by terminal deoxynucleotidyltransferase (TDT)-mediated dUTP-biotin nick end labeling assay (control: 0.00 + 0.00, 0.01%: 3.48 + 2.18, 0.02%: 9.64 + 5.75, 0.04%: 17.72 + 6.26, and 0.08%: 14.60 + 8.18 cells) and decrease in the viability of hair cell mitochondria by 2-(4-[dimethylamino] styryl)-N-ethylpyridinium iodide assay (control: 9.61 + 1.47, 0.01%: 8.28 + 2.22, 0.02%: 8.45 + 2.72, 0.04%: 7.25 + 2.44, and 0.08%: 6.77 + 3.26 percentage of total area) were observed. Antifog agent exposure can cause hair cell damage in zebrafish larvae, possibly by induction of mitochondrial damage with subsequent apoptosis of hair cells.

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Ecabet sodium alleviates neomycin-induced hair cell damage

Cited by 12 publications

References 45 publications

Preventive effect of ecabet sodium on low-dose aspirin-induced small intestinal mucosal injury: a randomized, double-blind, pilot study

Preventive effect of ecabet sodium on low-dose aspirin-induced small intestinal mucosal injury: a randomized, double-blind, pilot study

Impact of Nicotine Exposure on Hair Cell Toxicity and Embryotoxicity During Zebrafish Development

Evaluation of Ototoxicity of an Antifog Agent and the Suspected Underlying Mechanisms: An Animal Study

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