Ecdysone signaling in adult Drosophila melanogaster

Schwedes, Christoph Cleland; Carney, Ginger E.

doi:10.1016/j.jinsphys.2012.01.013

Cited by 135 publications

(92 citation statements)

References 91 publications

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“…S3A). No ecdysteroids were detected in adult males or females, consistent with extremely low RIA measurements from adults (reviewed by Schwedes and Carney, 2012). MaA is consistently about twofold more abundant than 20E throughout development, but their profiles peak at the same times, confirming that neither is a precursor for the other.…”

Section: The Ecdysteroidome In Embryonic Larval and Pupal Developmentsupporting

confidence: 73%

The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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Ecdysteroids are the hormones regulating development, physiology and fertility in arthropods, which synthesize them exclusively from dietary sterols. But how dietary sterol diversity influences the ecdysteroid profile, how animals ensure the production of desired hormones and whether there are functional differences between different ecdysteroids produced in vivo remains unknown. This is because currently there is no analytical technology for unbiased, comprehensive and quantitative assessment of the full complement of endogenous ecdysteroids. We developed a new LC-MS/MS method to screen the entire chemical space of ecdysteroid-related structures and to quantify known and newly discovered hormones and their catabolites. We quantified the ecdysteroidome in Drosophila melanogaster and investigated how the ecdysteroid profile varies with diet and development. We show that Drosophila can produce four different classes of ecdysteroids, which are obligatorily derived from four types of dietary sterol precursors. Drosophila makes makisterone A from plant sterols and epi-makisterone A from ergosterol, the major yeast sterol. However, they prefer to selectively utilize scarce ergosterol precursors to make a novel hormone 24,28-dehydromakisterone A and trace cholesterol to synthesize 20-hydroxyecdysone. Interestingly, epi-makisterone A supports only larval development, whereas all other ecdysteroids allow full adult development. We suggest that evolutionary pressure against producing epi-C-24 ecdysteroids might explain selective utilization of ergosterol precursors and the puzzling preference for cholesterol.

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Section: The Ecdysteroidome In Embryonic Larval and Pupal Developmentsupporting

confidence: 73%

The Ecdysteroidome of Drosophila: influence of diet and development

et al. 2015

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“…On the other hand, insects evidently can use ecdysteroids in similar ways that vertebrates use estrogens, including e.g., stimulation of oocyte growth and maturation (De Loof 2006, De Loof et al 2016. This is consistent with the widespread expression of EcR protein in adult insect tissues -including gonads and CNS (Schwedes & Carney 2012) -and lack of an ER orthologue in the Drosophila genome (Boulanger & Dura 2015). Estrogenic stimulation of oocyte growth and vitellogenesis in experimental crabs -also devoid of ERhas been explained by estradiol binding to EcR, based on in silico ligand docking experiments (Swetha et al 2016).…”

Section: Sex Steroid Surrogatessupporting

confidence: 66%

“…It is a defining feature of animals with a rigid exoskeleton, which is split and shed to accommodate serial phases of growth and development (Nijhout & Callier 2015) (Box 2). The process depends on moult-inducing steroids secreted by the prothoracic gland as metabolites of dietary cholesterol (Schwedes & Carney 2012). The first moult-inducing steroid to be identified -ecdysone -was crystallized by none other than Adolf Butenandt (Butenandt & Karlson 1954 (Fig.…”

Section: :3mentioning

confidence: 99%

On gonads and gadflies: the estrus angle

Hillier

2017

Journal of Endocrinology

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The first sex steroid to be crystallized was the vertebrate ovarian hormone, estrone -a less potent metabolite of 17β-estradiol, which in mammals stimulates the female urge to mate (estrus). The gadfly (Greek oistros) lent its name to the process of estrus, as an insect that bites and torments in classical Greek mythology. With the purification and crystallization of a moult-inducing steroid (ecdysone) from insects, an interesting parallel emerged between mating and moulting in lower mammals and arthropods. Ecdysterone (potent ecdysone metabolite) has anabolic effects in mammalian muscle cells that can be blocked by selective estrogen receptor antagonists. Insects utilize ecdysteroids in similar ways that vertebrates use estrogens, including stimulation of oocyte growth and maturation. Ecdysteroids also modify precopulatory insect mating behaviour, further reinforcing the gonad-gadfly/mate-moult analogy.

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“…Therefore, higher SpEcR mRNA level detected in the ovary of S. paramamosain in this study probably indicated that they were involved in the regulation of ovarian development and reproduction. In fact, in the ovary of fruit fly D. melanogaster, EcR has been reported to regulate the transcripts of a set of genes, including ecdysone-induced protein 75B and 74, early in the ecdysone signal pathway and the expressions of these genes in turn regulated the development/degeneration of the immature eggs (Schwedes & Carney 2012). Ye et al (2010) also reported that in S. paramamosain, the yolk protein accumulated in parallel with ovarian maturation.…”

Section: Discussionmentioning

confidence: 99%

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

Gong¹,

Ye²,

Xie³

et al. 2015

Journal of Endocrinology

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In arthropods, it is known that ecdysteroids regulate molting, limb regeneration, and reproduction through activation of the ecdysone receptor (EcR). However, the ecdysteroid signaling pathway for promotion of ovarian development in crustaceans is still unclear. In this study, three cDNA isoforms of EcR were cloned from the mud crab Scylla paramamosain. qRT-PCR revealed that the SpEcR mRNA was abundant in the eyestalk, ovary and epidermis. During ovarian development, the SpEcR transcripts increased from stage I (undeveloped stage) and reached a peak at stage IV (late vitellogenic stage) before dropping to a lower level at stage V (mature stage). Meanwhile, levels of 20-hydroxyecdysone (20E) in the hemolymph, detected by HPLC-MS, displayed a similar pattern of increase with ovarian development. Results from in situ hybridization indicated that SpEcR mRNA was present in the follicular cells during vitellogenesis. Results from in vivo experiments revealed that 20E at 0.2 mg/g body weight significantly stimulated the expression of SpEcR and vitellogenin (SpVg) in female crabs during the early vitellogenic stage but not during the previtellogenic stage. This was confirmed by results from in vitro experiments which indicated that SpEcR and SpVg expression levels were significantly upregulated in early vitellogenic ovarian explants incubated with 5.0 mM 20E at 3 and 6 h but not in previtellogenic ovarian explants. Finally, results from in vitro gene silencing experiments indicated that the expression of SpEcR and SpVg in the ovary was significantly inhibited by SpEcR dsRNA. All these results together indicated that in S. paramamosain, 20E, and SpEcR, located in the follicular cells, play important roles in the promotion of ovarian development via regulating the expression of SpVg.

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Ecdysone signaling in adult Drosophila melanogaster

Cited by 135 publications

References 91 publications

The Ecdysteroidome of Drosophila: influence of diet and development

The Ecdysteroidome of Drosophila: influence of diet and development

On gonads and gadflies: the estrus angle

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

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