Ecdysone signaling promotes expression of multifunctional RNA binding proteins essential for ovarian germline stem cell self-renewal in<i>Drosophila</i>

Ds, Finger; Vv, Holt; Ables, Elizabeth T.

doi:10.1101/321109

Cited by 2 publications

(1 citation statement)

References 96 publications

(141 reference statements)

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“…These cells also show a low level of expression from the mCherry uorescent marker, preventing them from being removed during germline cell ltering via Fluorescence activated cell sorting (FACS). A signi cant enrichment for expression of geminin (AGAP000496) (Log2FC: 2.71, p = 0.002) and squid (AGAP000399) (Log2FC: 3.96, p < 0.0001), genes involved in the maintenance GSCs [25][26][27][28] , de ned the GSC cell cluster (Fig. 3c,d).…”

Section: Resultsmentioning

confidence: 99%

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Page

Galizi

Taxiarchi

et al. 2023

Preprint

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Understanding of the Anopheles gambiae germline has been essential to acquiring effective genetic control strategies targeting this malaria mosquito vector. This includes targeting germline genes to induce sterility or using regulatory sequences to drive transgene expression for applications such as gene drive. However, only very few germline regulatory elements have been characterised with the majority showing leaky expression. This has been shown to significantly reduce the efficiency of current genetic control strategies, which rely on regulatory elements that are more tightly restricted in their spatial and/or temporal expression. Additionally, meiotic silencing of the sex chromosomes during spermatogenesis limits the flexibility of transgene expression for design of novel genetic control strategies. In this work, we build on our previous study that dissected gametogenesis into four distinct cell populations. We used single-cell RNA sequencing to further discriminate these populations and define distinct germline cell-types. In doing so, we revealed an overexpression of X-linked genes in the germline stem cells (GSCs) and were able to pinpoint the onset of meiotic silencing of the X chromosome in the spermatogonia/primary spermatocytes. This study provides a comprehensive dataset to identify genes expressed at specific stages of spermatogenesis, widening the toolkit for genetic control of malaria mosquitoes.

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Section: Resultsmentioning

confidence: 99%

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Page

Galizi

Taxiarchi

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome

et al. 2023

View full text Add to dashboard Cite

Understanding development and genetic regulation in the Anopheles gambiae germline is essential to engineer effective genetic control strategies targeting this malaria mosquito vector. These include targeting the germline to induce sterility or using regulatory sequences to drive transgene expression for applications such as gene drive. However, only very few germline-specific regulatory elements have been characterised with the majority showing leaky expression. This has been shown to considerably reduce the efficiency of current genetic control strategies, which rely on regulatory elements with more tightly restricted spatial and/or temporal expression. Meiotic silencing of the sex chromosomes limits the flexibility of transgene expression to develop effective sex-linked genetic control strategies. Here, we build on our previous study, dissecting gametogenesis into four distinct cell populations, using single-cell RNA sequencing to define eight distinct cell clusters and associated germline cell–types using available marker genes. We reveal overexpression of X-linked genes in a distinct cluster of pre-meiotic cells and document the onset of meiotic silencing of the X chromosome in a subcluster of cells in the latter stages of spermatogenesis. This study provides a comprehensive dataset, characterising the expression of distinct cell types through spermatogenesis and widening the toolkit for genetic control of malaria mosquitoes.

show abstract

Ecdysone signaling promotes expression of multifunctional RNA binding proteins essential for ovarian germline stem cell self-renewal inDrosophila

Cited by 2 publications

References 96 publications

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Single-cell profiling of mosquito spermatogenesis defines the onset of meiotic silencing and pre-meiotic overexpression of the X chromosome.

Single-cell profiling of Anopheles gambiae spermatogenesis defines the onset of meiotic silencing and premeiotic overexpression of the X chromosome

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