Echinacea: Cultivation and Medicinal Value

Li, Thomas S. C.

doi:10.21273/horttech.8.2.122

Cited by 58 publications

(59 citation statements)

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“…As for the diploid plants, they might have originated from the other parts of the anthers such as from the residues of the filament tissues and are heterozygous (Li 1998), or through spontaneous chromosome doubling of haploids and are homozygous. Clarifying their origins requires further experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Purple coneflower is generally cross-pollinated (Li 1998), but the mechanism for protection against self-pollination is by protandry and is not self-sterile (Sejdler-Lozykowska and Dabrowska 1996). The present success in regeneration of haploid plants by anther culture enables us to put forwards an ambitious breeding program for the production of hybrid vigor seeds for this important medicinal plant.…”

Section: Discussionmentioning

confidence: 99%

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Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.)

Zhao

Dahanayake

Yang

et al. 2006

Plant Cell Tiss Organ Cult

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Anthers containing microspores at the uninucleate stage were excised from capitula of field grown plants of purple coneflower, Echinacea purpurea, and cultured on medium conducive to callus growth. In callus induction cultures, N6 basal medium was more effective than Murashige and Skoog (MS), and a combination of benzyladenine (BA) at 2.22 lM with naphthaleneacetic acid (NAA) at 0.054 lM was more effective than 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.52, 9.05 and 13.57 lM. Callus induction rate as high as 85.8% was achieved, and no statistically significant differences were found among cultures with various densities of 20, 40, 60, 80 and 100 anthers per bottle each filled with 40-ml medium. Shoots were regenerated from calluses on MS medium containing 2.22 lM BA and various concentrations of NAA, with the highest regeneration rate of 95.24% obtained when 0.27 lM NAA was applied. Although a large portion of the regenerated shoots had prominent symptom of vitrification, some normal shoots could be easily rooted on MS medium containing 0.054 lM NAA. Thirty regenerated plants were randomly selected and 19 of them were confirmed to be haploid by observation of chromosome number of root-tip cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.)

Zhao

Dahanayake

Yang

et al. 2006

Plant Cell Tiss Organ Cult

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“…However, the efficiency of Echinacea seed germination and transplant production is rather low and inconsistent, ranging from no germination to variable frequency, depending on the physiology of the seed and the growth environment, soil pH, and moisture (Hobbs 1998;Macchia et al 2001). Echinacea seed dormancy also varies with species (Li 1998). E. pallida and E. angustifolia exhibit higher levels of dormancy than E. purpurea (Hobbs 1998).…”

Section: Conventional Propagation Practicesmentioning

confidence: 99%

Echinacea biotechnology: Challenges and opportunities

Abbasi

Saxena

Murch

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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Echinacea, better known as purple coneflower, has received a global attention because of its increasing medicinal value. There is enormous potential for the discovery of new medicinal compounds in this species and an immediate need for techniques to facilitate the production of high quality, chemically consistent plant material for drug development and clinical trials. In vitro tissue culture of Echinacea can play a vital role in the development of novel germplasm, rapid multiplication, and genetic modifications for an enhanced phytochemical production. Recent establishment of liquid culture techniques, large-scale bioreactors, and Agrobacterium-mediated transformation are changing the production parameters of the Echinacea species. This review provides an overview of the recent developments in in vitro technologies and challenges that remain in the Echinacea biotechnology.

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“…Extracts from Echinacea are used as phytomedicines to treat colds, flu (Letchamo et al 2002), wound infections (Bauer and Wagner 1991), and as an immunostimulant and anti-inflammatory compound . The demand for plant material from this genus has increased in recent years, prompting concern for the status of native populations and the potential loss of genetic diversity (Li 1998;Widrlechner and McKeown 2002).…”

Section: Introductionmentioning

confidence: 99%

Alkamide production from hairy root cultures of Echinacea

Romero

Delate

Kraus

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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Hairy root cultures of Echinacea, one of the most important medicinal plants in the US, represent a valuable alternative to field cultivation for the production of bioactive secondary metabolites. In this study, the three most economically important species of Echinacea (Echinacea purpurea, Echinacea pallida, and Echinacea angustifolia) were readily transformed with two strains of Agrobacterium that produce the hairy root phenotype. Transformed roots of all three species exhibited consistent accelerated growth and increased levels of alkamide production. Optimization of the culture of Echinacea hairy roots was implemented to enhance both growth and alkamide production concomitantly. The use of halfstrength Gamborg's B5 medium supplemented with 3.0% sucrose was twice as effective in maintaining hairy root production than any other media tested. The addition of indolebutyric acid increased the growth rate of roots by as much as 14-fold. Alkamide production increased severalfold in response to the addition of the elicitor, jasmonic acid, but did not respond to the addition of indolebutyric acid. Induced accumulation of the important bioactive compounds, alkamides 2 and 8, was observed both in transformed roots and in response to jasmonic acid treatments. The results of this study demonstrate the efficacy of hairy root cultures of Echinacea for the in vitro production of alkamides and establish guidelines for optimum yield.

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Echinacea: Cultivation and Medicinal Value

Cited by 58 publications

References 0 publications

Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.)

Anther culture and haploid plant regeneration in purple coneflower (Echinacea purpurea L.)

Echinacea biotechnology: Challenges and opportunities

Alkamide production from hairy root cultures of Echinacea

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