Echinostoma and Echinostomiasis

Huffman, Jane E.; Fried, Bernard

doi:10.1016/s0065-308x(08)60107-4

Cited by 213 publications

(156 citation statements)

References 95 publications

Supporting

Mentioning

154

Contrasting

Order By: Relevance

“…Most of the worms recovered from the DEX-treated mice on clay 12 p. i. were gravid adults in contrast to immature juveniles from the control mice. This finding is useful for raising gravid worms in immunologically suppressed mice to run the life cycle of E. trivolvis in the laboratory-reared llelisoma trivolvis snails (see review in Huffman & Fried, 1990 resistance to secondary and superimposed infections in mice. Experimental Parasitology, 1986, 67, 311-318. …”

Section: Discussionmentioning

confidence: 99%

The expulsion ofEchinostoma trivolvis: suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice

et al. 1996

View full text Add to dashboard Cite

Summary :C3H/HeN mice were infected with Echinostoma trivolvis metacercariae on day 0, given intramuscular injections of dexamethasone daily for 5 or 7 days, and necropsied on days 5, 8, 12, 1 5, 20 and 30 p. i. Controls consisted of mice that were infected with echinostomes, but were not treated with dexamethasone. Dexamethasone treatment caused a delay in worm expulsion from the small intestine of the hosts, and the increase in goblet cell numbers that occurred in untreated mice was markedly delayed in the treated mice. Mast cell number in the small intestine increased rapidly from just after day 5 p. i. and reached a peak on day 15 p. i. in both dexamethasone-treated and control mice, although the increase in cell numbers was delayed slightly in the dexamethasone-treated mice. The eosinophi number in the small intestine of dexamethasone-treated mice was suppressed until 8 days p. i. and then increased reaching a peak on day 1 2 p. i., although the number was about one half that of the control. As determined on day 1 2 p. i., the mean body area of worms from dexamethasone-treated animals was significantly greater than that of the controls. Histological examination of the small intestine showed that the goblet and Paneth cell hyperplasia seen in mice infected with E. trivolvis was suppressed by dexamethosone-treatment. Transmission electron microscopy revealed no marked ultrastructural differences in the small intestine of the dexamethasone-treated and control mice except that the former had an increased number of intracristal granules in mitochondria, an increase in vesicles in the apical epithelial cells and an increase in amorphous bodies and autophagic vacuoles in the Paneth cells. These results indicate that dexamethasone treatment delayed the expulsion of E. trivolvis from the small intestine of the host mouse in association with the suppression of goblet cell hyperplasia and increase in the number of mast cells and eosinophils.KEY WORDS : Echinostoma trivolvis, dexamethasone, gobl et cell hyperplasia, worm rejection, C3H/HeN mi ce. Résumé : L'EXPULSION DE ECHINOSTOMA TRIVOLVIS : EFFETS SUPPRESSEURS DE LA DEXAMÉTHASONE SUR L'HYPERPLASIE DES CELLULES CALICIFORMES ET L' ÉLIMINATION DES VERS CHEZ LA SOURIS C3H/HEN Des souris C3H/HeN ont été infectées à JO avec des métacercaires d'Echinostoma trivolvis, ont reçu quotidiennement de la dexaméthasone en intra-musculaire pendant 5 à 7 jours et ont été nécropsiées à J5, J8, J12, J15, J20 et J30. Des souris témoins ont été infectées par les échinostomes, mais n'ont pas été traitées par la dexaméthasone. Le traitement par la dexamethasone a provoqué un retard dans l'expulsion intestinale des vers par l'hôte, et l'augmentation du nombre des cellules caliciformes observé chez les souris non traitées a été nettement retardée chez les souris traitées. Le nombre de mastocytes intestinaux a rapidement augmenté juste après J5 et atteint un pic à J15 chez les souris traitées comme chez les témoins, bien que l'augmentation du nombre de cellules oit été légèrement retardée c...

show abstract

Section: Discussionmentioning

confidence: 99%

The expulsion ofEchinostoma trivolvis: suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice

et al. 1996

View full text Add to dashboard Cite

show abstract

“…At least 30 genera and more than 200 species of this family are known in the world, 15 species are reported to infect humans (Chai et al 2005a;Huffman and Fried 1990). Among them, 10 species were reported as fish-borne echinostome species (Table I).…”

Section: Echinostomatidaementioning

confidence: 99%

Global status of fish-borne zoonotic trematodiasis in humans

Hùng

Madsen

Fried

2013

Acta Parasitologica

Self Cite

111

View full text Add to dashboard Cite

Fishborne zoonotic trematodes (FZT), infecting humans and mammals worldwide, are reviewed and options for control discussed. Fifty nine species belonging to 4 families, i.e. Opisthorchiidae (12 species), Echinostomatidae (10 species), Heterophyidae (36 species) and Nanophyetidae (1 species) are listed. Some trematodes, which are highly pathogenic for humans such as Clonorchis sinensis, Opisthorchis viverrini, O. felineus are discussed in detail, i.e. infection status in humans in endemic areas, clinical aspects, symptoms and pathology of disease caused by these flukes. Other liver fluke species of the Opisthorchiidae are briefly mentioned with information about their infection rate and geographical distribution. Intestinal flukes are reviewed at the family level. We also present information on the first and second intermediate hosts as well as on reservoir hosts and on habits of human eating raw or undercooked fish.

show abstract

“…A recent study in our laboratory using daughter rediae of the ubiquitous North American digenetic trematode Echinostoma trivolvis (see review in Huffman & Fried, 1990) showed that these rediae are attracted to each other in vitro and that lipids released from them are chemoattractants (Reddy & Fried, 1996). The purpose of this study was to identify the lipid classes released by rediae of E. trivolvis into a bioassay medium and to determine which lipids are chemoattractive.…”

Section: Introductionmentioning

confidence: 99%

Chemoattraction ofEchinostoma trivolvis(Trematoda) rediae to lipophilic excretory-secretory products and thin layer-chromatographic analysis of redial lipids

Reddy¹,

Frazer²,

Fried³

et al. 1997

Parasite

View full text Add to dashboard Cite

Summary :High performance thin-layer chromatography (HPTLC] was used to analyze the lipophilic excretory-secretory (ES) products of Echinostoma trivolvis rediae. These products were free sterols at a concentration of 0.83 ± 0.03 μg/100 ml and a lesser amount of free fatty acid that was not quantified. Preparative layer chromatography (PLC) was used to obtain the free sterol and free fatty acid fractions of redial ES products, and silica gel squares containing these lipids were tested against single rediae in a Petri dish bioassay. Rediae were significantly attracted to squares containing either free sterol or free fatty acid. HPTLC was also used to analyze the major lipid fractions in the redial bodies, which were free sterols, phosphatidylcholine (PC), and phosphatidylethanolamine |PE). The major wet weight percentage ± SE (n = 3) of free sterols was 2.59 ± 0.16 and for PC and PE was 0.01 1 ± 0.002 and 0.010 ± 0.002, respectively. KEY WORDS : Echinostoma trivolvis,

show abstract

Echinostoma and Echinostomiasis

Cited by 213 publications

References 95 publications

The expulsion ofEchinostoma trivolvis: suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice

The expulsion ofEchinostoma trivolvis: suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice

Global status of fish-borne zoonotic trematodiasis in humans

Chemoattraction ofEchinostoma trivolvis(Trematoda) rediae to lipophilic excretory-secretory products and thin layer-chromatographic analysis of redial lipids

Contact Info

Product

Resources

About