Echinostoma caproni: Identification of enolase in excretory/secretory products, molecular cloning, and functional expression

Marcilla, Antonio; Pérez-García, Ana; Espert, Ana; Bernal, Dolores; Muñoz-Antolí, Carla; Esteban, J. Guillermo; Toledo, Rafael

doi:10.1016/j.exppara.2007.03.011

Cited by 45 publications

(37 citation statements)

References 29 publications

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“…They showed that S. mansoni specifically bound 8 lectins, while E. paraensei bound only six out of the 11 lectins evaluated, with a distinct response in both species parasitizing the same intermediate host (Uchikawa and Loker 1991) . More recently, we have reported the identification of enolase as a plasminogen-binding molecule present in the ESP of E. caproni , suggesting its role as an interacting molecule with the host extracellular matrix (Marcilla et al 2007) .…”

Section: Identification Of Echinostome Proteinsmentioning

confidence: 96%

“…Using various heterologous antibodies Richard et al (1989) identified P granules in Echinostoma caproni (Richard et al 1989) . More recent studies include the localization of proteins tubulin and actin in E. caproni (Iomini and Justine 1997 ;Iomini et al 1998 ;Sebelová et al 2004) ; the identification of tubulin in E. paraensei (Pinheiro et al 2004) ; the identification of Sm14-like molecules in E. paraensei (Thaumaturgo et al 2002) ; as well as the identification of different proteins in E. friedi (Bernal et al 2006) or in E. caproni (Marcilla et al 2007 ;Guillou et al 2007) .…”

Section: Identification Of Echinostome Proteinsmentioning

confidence: 98%

“…Few exceptions include the enolase gene, originally identified in studies characterizing spliced leader RNAs in flatworms (Davies 1997) . The fulllength molecule has been recently cloned from mRNA obtained either from adults or sporocysts (Marcilla et al 2007 ;Guillou et al 2007) .…”

Section: Echinostome Genomicsmentioning

confidence: 99%

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Echinostomes: genomics and proteomics

Marcilla

2008

The Biology of Echinostomes

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Echinostomes are excellent models to study host-parasite interactions in intestinal helminthiases. In this chapter, data available from genomic and proteomic studies are analyzed in detail to indicate advantages and pitfalls in the research using these organisms. The lack of a genome sequence project and the low number of sequences deposited in the databases not only affect the genomic studies but also debilitate proteomic findings since in most cases, although good spectrometric data can be obtained, the available database often fail to identify the protein from the peptides obtained. A. Marcilla ( )

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Section: Identification Of Echinostome Proteinsmentioning

confidence: 96%

Section: Identification Of Echinostome Proteinsmentioning

confidence: 98%

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Echinostomes: genomics and proteomics

Marcilla

2008

The Biology of Echinostomes

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“…Recently, Muñoz-Antoli et al (2014) reported the regeneration of the intestinal tissue as a major effector mechanism responsible for the early expulsion of echinostomes and, probably, other intestinal helminths. Apart from these results, this group also has characterized several antigenic proteins and post-traslational modifications that can modulate the outcome of the infection (Bernal et al, 2006;Marcilla et al, 2007;Higón et al, 2008;Sotillo et al, 2008Sotillo et al, , 2010bSotillo et al, , 2014Muñoz-Antoli et al, 2014). Recently, Garg et al, (2013) presented the first transcriptome of the adult stage of an echinostome and compared it to those of other trematodes revealing similarities in transcription for molecules inferred to have key roles in parasite-host interactions.…”

Section: Late 20 Th Century and Early 21 Th Centurymentioning

confidence: 99%

History of echinostomes (Trematoda)

Toledo

Radev

Kanev

et al. 2014

Acta Parasitologica

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Echinostomatidae (Trematoda) is the largest family within the class Trematoda. Members of this family have been studied for many years in relation to their utility as basic research models in biodiversity and systematics and also as experimental models in parasitology since they offer many advantages. Echinostomes have contributed significantly to numerous developments in many areas studied by parasitologists and experimental biologists. In this review, we examine the history of the echinostomebased studies from the beginnings to the present. For this purpose, we have divided the history of echinostomes into four periods (i.e. 18 th and 19 th centuries, first half of the 20 th century, second half of the 20 th century and the late 20 th and 21 th century) according to the types of studies performed in each of them. Moreover, we also briefly review the history of echinostome infections in humans.

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“…Consequently the majority of efforts have focussed on testing the enzyme's potential as a vaccine candidate [157][158][159][160][161][162][163][164][165][166]. Silencing of the enolase gene in C. sinensis by RNAi resulted in significantly decreased survival of the worms, suggesting that the protein has potential as a drug target [167].…”

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confidence: 99%

Metabolic enzymes of helminth parasites: potential as drug targets

Timson¹

2015

CPPS

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Metabolic pathways which extract energy from carbon compounds are essential for an organism's survival. Therefore, inhibition of enzymes in these pathways represents a potential therapeutic strategy to combat parasitic infections. However, the high degree of similarity between host and parasite enzymes makes this strategy potentially difficult. Nevertheless, several existing drugs to treat infections by parasitic helminths (worms) target metabolic enzymes. These include the trivalent antimonials which target phosphofructokinase and Clorsulon which targets phosphoglycerate mutase and phosphoglycerate kinase. Glycolytic enzymes from a variety of helminths have been characterised biochemically, and some inhibitors identified. To date none of these inhibitors have been developed into therapies. Many of these enzymes are externalised from the parasite and so are also of interest in the development of potential vaccines. Less work has been done on tricarboxylic acid cycle enzymes and oxidative phosphorylation complexes. Again, while some inhibitors have been identified none have been developed into drug-like molecules. Barriers to the development of novel drugs targeting metabolic enzymes include the lack of experimentally determined structures of helminth enzymes, lack of direct proof that the enzymes are vital in the parasites and lack of cell culture systems for many helminth species. Nevertheless, the success of Clorsulon (which discriminates between highly similar host and parasite enzymes) should inspire us to consider making serious efforts to discover novel anthelminthics which target metabolic enzymes.

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Echinostoma caproni: Identification of enolase in excretory/secretory products, molecular cloning, and functional expression

Cited by 45 publications

References 29 publications

Echinostomes: genomics and proteomics

Echinostomes: genomics and proteomics

History of echinostomes (Trematoda)

Metabolic enzymes of helminth parasites: potential as drug targets

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