Disadvantage of ultrasonography is its dependence on subjective assessment of displayed images. The way how to minimize both intraobserver and interobserver differences is creation of standard conditions for examination including a quantitative approach to evaluation of tissue reflectivity. The oldest mode of standardisation is standardised A- scan, used in ophthalmology. It enables differentiation of echoes, reflected from different ocular structures and is helpful in assessment of extraocular muscle thickness. Standardisation of B- scan depends on the type of diagnostic device and is based on quantification of image echogenicity. In our study reference values of grey-level histogram were established for some thyroid diseases using standard setting of imaging parameters. Results indicate that both standardised A- and B- scan should be helpful in differential diagnostics.