ECM-based microchannel for culturing <i>in vitro</i> vascular tissues with simultaneous perfusion and stretch

Shimizu, Akira; Goh, Wei Huang; Itai, Shun; Hashimoto, Michinao; Miura, Shigenori; Onoe, Hiroaki

doi:10.1039/d0lc00254b

Cited by 41 publications

(33 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shimizu et al. presented an ECM‐based microsystem for developing 3D vascular tissues in vitro to mimic the blood vessels in tumor microenvironment [83]. This microchannel was fabricated with transglutaminase cross‐linked gelatin.…”

Section: Biophysical Factors In Tumor Microenvironmentmentioning

confidence: 99%

Recent research advances of the biomimetic tumor microenvironment and regulatory factors on microfluidic devices: A systematic review

Cheng

2022

Electrophoresis

View full text Add to dashboard Cite

Tumor microenvironment is a multicomponent system consisting of tumor cells, noncancer cells, extracellular matrix, and signaling molecules, which hosts tumor cells with integrated biophysical and biochemical elements. Because of its critical involvement in tumor genesis, invasion, metastasis, and resistance, the tumor microenvironment is emerging as a hot topic of tumor biology and a prospective therapeutic target. Unfortunately, the complex of microenvironment modeling in vitro is technically challenging and does not effectively generalize the local tumor tissue milieu. Recently, significant advances in microfluidic technologies have provided us with an approach to imitate physiological systems that can be utilized to mimic the characterization of tumor responses with pathophysiological relevance in vitro. In this review, we highlight the recent progress and innovations in microfluidic technology that facilitates the tumor microenvironment study. We also discuss the progress and future perspective of microfluidic bionic approaches with high efficiency for the study of tumor microenvironment and the challenges encountered in cancer research, drug discovery, and personalized therapy.

show abstract

Section: Biophysical Factors In Tumor Microenvironmentmentioning

confidence: 99%

Recent research advances of the biomimetic tumor microenvironment and regulatory factors on microfluidic devices: A systematic review

Cheng

2022

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…This system was shown to recapitulate nitric oxide production and reactive oxygen species production in response to hypertensive (18%) radial strain. In another study that combined both mechanical stretch and shear stress in their tissue chip system, a 3D printed sacrificial mold embedded in a gelatin-based solution formed ∼600 µm-wide microchannels (Shimizu et al, 2020) that were endothelialized with HUVECs and perfused with media. Periodic cyclic stretch of 10% was applied to the microchannel, concomitant with 2 dyn/cm 2 shear stress.…”

Section: External Mechanical Cuesmentioning

confidence: 99%

Engineering Cardiovascular Tissue Chips for Disease Modeling and Drug Screening Applications

Chan

Huang

2021

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

In recent years, the cost of drug discovery and development have been progressively increasing, but the number of drugs approved for treatment of cardiovascular diseases (CVDs) has been limited. Current in vitro models for drug development do not sufficiently ensure safety and efficacy, owing to their lack of physiological relevance. On the other hand, preclinical animal models are extremely costly and present problems of inaccuracy due to species differences. To address these limitations, tissue chips offer the opportunity to emulate physiological and pathological tissue processes in a biomimetic in vitro platform. Tissue chips enable in vitro modeling of CVDs to give mechanistic insights, and they can also be a powerful approach for drug screening applications. Here, we review recent advances in CVD modeling using tissue chips and their applications in drug screening.

show abstract

“…Biodegradable materials were also employed to construct hierarchical vascular networks, whose vascular widths ranged from 100 μm to 200 μm (Zhang et al, 2016). Furthermore, a recent study mimicked the complex geometries of vascular networks in vivo and fabricated complex microchannels with branches in a gelatin-based matrix using water-soluble sacrificial polyvinyl alcohol molds (Shimizu et al, 2020). Hierarchical vascular networks were generated whose vascular widths ranged from 200 μm to 3.2 mm.…”

Section: Microfluidic Pre-designed Modelsmentioning

confidence: 99%

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Watanabe

Sudo

2021

JBSE

View full text Add to dashboard Cite

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches IntroductionCurrently, organ transplantation is the most effective therapy for end-stage organ failure. However, the demand for life-saving organ transplants far exceeds the supply of available organs, including the kidney, liver, heart, and lung owing to organ shortage (Lauerer et al., 2016). To address this problem, tissue engineering has offered potential strategies for the construction of solid tissues and organs using cells, signaling factors, and scaffolds (Langer and Vacanti, 1993). Over the last several decades, various approaches have been developed for the construction of organ-specific tissues by utilizing cell culture platforms, such as porous scaffolds and decellularized matrices, and scaffold-free culture methods, including cell sheet engineering and spheroid culture (Uygun et al., 2010;Bao et al., 2011;No et al., 2012;Damania et al., 2014). Despite great progress, limitations remain for medical and clinical applications of tissue-engineered constructs owing to the lack of functional vascular networks, which may result in tissue dysfunction, such as hemorrhage, clot formation, and tissue hypoxia.Functional vascular networks should be constructed in tissue-engineered constructs. Establishing a hierarchical tubular network is required to supply oxygen and nutrients to the tissue constructs. In particular, following morphological

show abstract

ECM-based microchannel for culturing in vitro vascular tissues with simultaneous perfusion and stretch

Abstract: A perfusable and stretchable gelatin-based microfluidic system that can apply both simultaneous fluidic shear stress and stretch stress to in vitro endothelial 3D tissues is presented.

Cited by 41 publications

References 39 publications

Recent research advances of the biomimetic tumor microenvironment and regulatory factors on microfluidic devices: A systematic review

Recent research advances of the biomimetic tumor microenvironment and regulatory factors on microfluidic devices: A systematic review

Engineering Cardiovascular Tissue Chips for Disease Modeling and Drug Screening Applications

Progress and challenges in vascular tissue engineering using self-organization/pre-designed approaches

Contact Info

Product

Resources

About