ECM-Body: A Cell-Free 3D Biomimetic Scaffold Derived from Intact Planarian Body

Sonpho, Ekasit; Wootthichairangsan, Chanida; Ishida, Miyuki; Inoue, Takeshi; Agata, Kiyokazu; Maleehuan, Anchuleerat; Charngkaew, Komgrid; Chomanee, Nusara; Moonsom, Saengduen; Wongtrakoongate, Patompon; Chairoungdua, Arthit; Ounjai, Puey

doi:10.2108/zs190135

Cited by 4 publications

(9 citation statements)

References 41 publications

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“…Therefore, we explored detergent-based tissue decellularization at low temperature for isolating the extracellular matrix in S. mediterranea by incubating planarians with a low concentration of SDS at 4°C (Figure 1B). Compared our non-decellularized animals (Figure 1C), the results were similar to those from D. japonica (25) in that the decellularization without any pretreatment (NP-ECM) could maintain the animal's overall morphology, yet, the external structure of the decellularized animals was tremendously compromised (Figure 1D). Moreover, internal structures of decellularized animals were not well-defined.…”

Section: Resultssupporting

confidence: 69%

“…Moreover, internal structures of decellularized animals were not well-defined. As in D. japonica, pre-treating the planarians with formaldehyde prior to decellularization J o u r n a l P r e -p r o o f did preserve the external and internal integrity of isolated decellularized structure in S. mediterranea (Figure 1E) (25). The resultant decellularized animals (FA-ECM) had a translucent appearance with intact gross external structures and maintained the organization of internal organs such as the digestive tract branches, eye spots, and pharynx (Figure 1E).…”

Section: Resultsmentioning

confidence: 87%

“…for the isolation of ECM from S. mediterranea (25). Therefore, we explored detergent-based tissue decellularization at low temperature for isolating the extracellular matrix in S. mediterranea by incubating planarians with a low concentration of SDS at 4°C (Figure 1B).…”

Section: Resultsmentioning

confidence: 99%

“…Animal Decellularization-Whole-mount planarian decellularization has been optimized based on a previous publication (25). We optimized three protocols for decellularization, which we refer to as No Pretreatment ECM (NP-ECM), Formaldehyde ECM (FA-ECM), and N-Acetyl Cysteine pretreatment (NAC-ECM).…”

Section: Methodsmentioning

confidence: 99%

“…Similarly, Sonpho et al (25) successfully developed a simple technique for characterizing the morphology of J o u r n a l P r e -p r o o f isolated ECM by whole organism decellularization of a different planarian species. However, a complete systematic workflow for studying ECM biology in planarians has not yet been established.…”

Section: Introductionmentioning

confidence: 99%

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Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration

Sonpho¹,

Mann²,

Levy³

et al. 2021

Molecular & Cellular Proteomics

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 87%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration

Sonpho¹,

Mann²,

Levy³

et al. 2021

Molecular & Cellular Proteomics

Self Cite

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Biomaterial Design Inspired by Regenerative Research Organisms

Sapru

Dill

Simmons

2022

ACS Biomater. Sci. Eng.

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The efficacy of implanted biomaterials is largely dependent on the response of the host's immune and stromal cells. Severe foreign body response (FBR) can impede the integration of the implant into the host tissue and compromise the intended mechanical and biochemical function. Many features of FBR, including late-stage fibrotic encapsulation of implants, parallel the formation of fibrotic scar tissue after tissue injury. Regenerative organisms like zebrafish and salamanders can avoid fibrosis after injury entirely, but FBR in these research organisms is rarely investigated because their immune competence is much lower than humans. The recent characterization of a regenerative mammal, the spiny mouse (Acomys), has inspired us to take a closer look at cellular regulation in regenerative organisms across the animal kingdom for insights into avoiding FBR in humans. Here, we highlight how major features of regeneration, such as blastema formation, macrophage polarization, and matrix composition, can be modulated across a range of regenerative research organisms to elucidate common features that may be harnessed to minimize FBR. Leveraging a deeper understanding of regenerative biology for biomaterial design may help to reduce FBR and improve device integration and performance.

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Decellularization enables functional analysis of ECM remodeling in planarian regeneration

Sonpho

Mann

Levy

et al. 2020

Preprint

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Extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions. However, only few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration. We utilized a free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNA-interference (RNAi) to characterize and investigate ECM functions during tissue homeostasis and regeneration. High-quality ECM was isolated from planarians, and its matrisome profile was characterized by LC-MS/MS. The functions of identified ECM components were interrogated using RNAi. Using this approach, we discovered that heparan sulfate proteoglycan and kyphoscoliosis peptidase are essential for both tissue homeostasis and regeneration. Altogether, our strategy provides a robust experimental approach for identifying novel ECM components involved in regeneration that might not be discovered bioinformatically.

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ECM-Body: A Cell-Free 3D Biomimetic Scaffold Derived from Intact Planarian Body

Cited by 4 publications

References 41 publications

Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration

Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration

Biomaterial Design Inspired by Regenerative Research Organisms

Decellularization enables functional analysis of ECM remodeling in planarian regeneration

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