Eco-friendly and green chromatographic method for the simultaneous determination of chlorocresol and betamethasone dipropionate in topical formulations using Box–Behnken design

Muchakayala, Siva Krishna; Katari, Naresh Kumar; Dongala, Thirupathi; Marisetti, Vishnu Murthy; Vyas, Govind; Vegesna, Raju V. K.

doi:10.1007/s13738-021-02388-5

Cited by 45 publications

(24 citation statements)

References 33 publications

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“…Green Analytical Procedure Index (GAPI), the National Environmental Methods Index (NEMI), and analytical eco‐scale tools were used to determine the greenness of the method. Analytical eco‐scale was good for demonstrating the value of a method's eco‐friendliness by calculating a mathematical score (Gałuszka et al, 2012; Kurowska‐Susdorf et al, 2019; Mohamed & Fouad, 2020; Muchakayala, Katari, et al, 2021; Płotka‐Wasylka, 2018). Each negative effect on the environment results in a deduction of penalty points from a total of 100 points (which are high energy consumption, waste generation, and hazardous reagents) and gives the analytical eco‐score value.…”

Section: Resultsmentioning

confidence: 99%

A Quality by Design and green LC technique for the determination of mast cell stabilizer and histamine receptor antagonist (Olopatadine HCl) in multiple formulations

Kowtharapu

Katari

Surekha

et al. 2022

Biomedical Chromatography

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Mast cell stabilizer and histamine receptor antagonist olopatadine hydrochloride (OPT) assay method predicated on LC have been established for the analysis in multiple formulations. The current method dealt with ophthalmic solution, nasal spray, and tablet formulation products. The isocratic chromatography method was optimized and validated with a Boston green C8 column (150 Â 4.6 mm, 5 μm i.d.). Sodium dihydrogen phosphate buffer (pH 3.5) with acetonitrile in the ratio of 75:25 (v/v) was used as a mobile phase at a flow rate of 1.0 mL min À1 and at the column temperature of 30 C, and the detection was done at 299 nm. The method was validated as per International Council for Harmonisation (ICH) guidelines and United States Pharmacopoeia (USP). The accuracy results ranged from 99.9 to 100.7%, % relative standard deviation (RSD) from the precision was 0.5, and correlation coefficient from the linearity experiment was > 0.999. Solution stability was established for 24 h at room temperature and refrigerator conditions, and it was found that the solutions were stable. Using quality by design-based experiment designs, critical quality attributes were studied and it was found that the method was robust. In all the forced degradation studies peak purity was passed, and no interference was found at the retention time of the active component. The method validation data demonstrated that the developed method is linear, precise, accurate, specific, robust, and stable for the determination of OPT from multiple formulations. Analytical eco-scale tool, Green Analytical Procedure Index (GAPI) tool, and the National Environmental Method Index (NEMI) were used to evaluate the greenness of the method, and the analytical eco-score of 77 for the presented method was found to be excellent.

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Section: Resultsmentioning

confidence: 99%

A Quality by Design and green LC technique for the determination of mast cell stabilizer and histamine receptor antagonist (Olopatadine HCl) in multiple formulations

Kowtharapu

Katari

Surekha

et al. 2022

Biomedical Chromatography

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“…Because of the large number of structurally similar chemicals that must be isolated and monitored during the shelf-life of steroid drugs such as BD, the creation of a technique for specifying stability for This product is very difficult. Multiple methods of measuring betamethasone dipropionate and chlorocresol simultaneously or singly have been developed in the past, using a variety of instruments such as ultraviolet spectrometers, high performance liquid chromatography, high performance liquid chromatography with mass spectrometry and super performance liquid chromatography [15][16]. The results of this study, the current technique, established using RPHPLC to detect CRC and BTD in dosage form, was said to be easy, accurate, fast and cost-effective.…”

Section: Introductionmentioning

confidence: 94%

Development and Validation of the RP-HPLC Method for the Estimation of Chlorocresol and Betamethasone Dipropionate in Semisolid Dosage Formulation

Khatawakar¹,

Sakat²,

Inamdar³

et al. 2022

JPRI

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A simple, accurate, reproducible and cost-effective reverse phase HPLC technique for chlorocresol (CRC) as a preservative as well as betamethasone dipropionate (BTD) in bulk drug as well as semi-dose formulations. Snakes were developed during this research. In this method, Discovery HS (150x4.6 mm),was used as the stationary phase and an 80:20 mixture of water and isopropyl alcohol was used as the mobile phase A, and a mixture of acetonitrile plus tetrahydrofuran in the ratio. 70:30 ratio was used as mobile phase B in gradient mode to produce the final product. Approximately 1.00 ml/min of the solution is passed through the chromatograph by pumping it through. The UV detector operates at a wavelength of 240 nm. A validation study in accordance with the ICH Q2 (R1) guidelines is required to demonstrate that the new analytical method meets the reliability characteristics and these characteristics demonstrate the ability of a new analytical method. analysis in maintaining, over time, the basic validation criteria: selectivity, linearity, precision, precision, and specificity. When this method is used for quality control of commercial semi-solid dosage forms of betamethasone dipropionate throughout the day, the stability indicator technique can be used to measure the breakdown of the drug and its products it, as well as to evaluate the results of the tube homogeneity test.

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“…However, the secretion of myocardial proteins such as cardiac troponins does not necessarily entail the death of myocardial cells. In the absence of cell death, experiments suggest a variety of mechanisms for protein extrusion from reversibly damaged cardiomyocytes, include cell wounds that produce transient alterations in permeability, the formation of microparticles and their release [ [34] , [35] , [36] ]. In Petri dishes or animal models, cardiomyocytes that appear to be viable exchange macromolecules via the plasma membrane [ 37 , 38 ].…”

Section: Overview Of Cardiac Injurymentioning

confidence: 99%

Role of platelet rich plasma mediated repair and regeneration of cell in early stage of cardiac injury

Imam

Al‐Abbasi

Hosawi

et al. 2022

Regenerative Therapy

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Eco-friendly and green chromatographic method for the simultaneous determination of chlorocresol and betamethasone dipropionate in topical formulations using Box–Behnken design

Cited by 45 publications

References 33 publications

A Quality by Design and green LC technique for the determination of mast cell stabilizer and histamine receptor antagonist (Olopatadine HCl) in multiple formulations

A Quality by Design and green LC technique for the determination of mast cell stabilizer and histamine receptor antagonist (Olopatadine HCl) in multiple formulations

Development and Validation of the RP-HPLC Method for the Estimation of Chlorocresol and Betamethasone Dipropionate in Semisolid Dosage Formulation

Role of platelet rich plasma mediated repair and regeneration of cell in early stage of cardiac injury

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