Background: Japanese encephalitis (JE) is a dreadful disease transmitted by Culex vishnui group of mosquitoes. Control of JE vectors at the larval stage is one of the effective approaches in controlling JE. Methods: Leaves of Holoptelea integrifolia were subjected to petroleum ether, ethyl acetate, acetone and absolute alcohol solvent extraction by Soxhlet apparatus. As the ethyl acetate extract showed best mosquito larvicidal activity against Culex vishnui, it extract was selected and processed further for isolation of active principle through column chromatography and TLC and then characterization of the active principle by FTIR and GC-MS was done. Results: Ethyl acetate extract was found to be the most potent larvicide. In the active fraction of isolated compounds from ethyl acetate extract, N-methyl-1-adamantaneacetamide was the major constituent responsible for larvicidal activity of Culex vishnui. Green nanoparticles were synthesised by treating silver nitrate with leaf extract of H. integrifolia and were examined for larvicidal activity. Nanoparticles were characterised by UV-VIS spectral analysis, XRD study, TEM, SEM and FTIR spectral analysis. Synthesised nanoparticles were 40-50 nm in size and showed good larvicidal activity on Cx. vishnui mosquitoes at 1.25, 2.25, 5, 7.5 and 10 ppm concentrations. Active principle of plant extract and green nanoparticles showed eco-friendly effect on non-target organisms like Chironomus circumdatus, Daphnia sp, Diplonychus anulatum and Tadpole larvae. Conclusion: Thus it can be concluded that active ingredient as well as green synthesized nano particles from H. integrifolia can be a good alternative of presently used chemical insecticides.