Ecological Behavior ofLactobacillus reuteri100-23 Is Affected by Mutation of theluxSGene

Abstract

The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of the luxS mutation by a plasmid construct containing luxS restored AI-2 and AI-3 synthesis. In vitro experiments revealed that neither the growth rate, nor the cell yield, nor cell survival in the stationary phase were compromised in the luxS mutant relative to the wild type and complemented mutant. The ATP content of exponentially growing cells of the luxS mutant was, however, 65% of that of wild-type cells. Biofilms formed by the luxS mutant on plastic surfaces in a bioreactor were thicker than those formed by the wild type. Biofilm thickness was not restored to wild-type values by the addition of purified AI-2 to the culture medium. In vivo experiments, conducted with ex-Lactobacillus-free mice, showed that biofilms formed by the mutant strain on the epithelial surface of the forestomach were approximately twice as thick as those formed by the wild type. The ecological performance of the luxS mutant, when in competition with L. reuteri strain 100-93 in the mouse cecum, was reduced compared to that of a xylA mutant of 100-23C. These results demonstrate that LuxS influences important ecological attributes of L. reuteri 100-23C, the consequences of which are niche specific.The proximal region of the gastrointestinal tracts of rodents, pigs, chickens, and horses is inhabited by a Lactobacillus population. These bacteria adhere to, and proliferate on, the surface of nonsecretory epithelia present in the forestomachs, pars oesophagea, crops, and nonglandular stomachs of mice and rats, pigs, chickens, and horses, respectively (7,32,39). These epithelial associations can be termed biofilms because the bacteria appear to be encased in carbohydrate material and layers of bacterial cells several cells thick can be demonstrated in stained cryosections on the surface of proximal gut epithelia (7,24). Shed from these biofilms, lactobacilli can be detected throughout the gut of the animal, including in the large bowel (23).Lactobacillus reuteri strain 100-23 is an autochthonous inhabitant of the rodent gut. Recent molecular biological studies have begun to unravel the molecular mechanisms that enable this bacterial strain to reside in the guts of mice (34,35). These studies have shown that L. reuteri 100-23 and the murine gut together provide an excellent paradigm to study the ecologically important characteristics, at the molecular level, of a gram-positive bacterial species that can form an epitheliumassociated biofilm and of coping with ecological competition in the large bowel environment (34,35).Several examples of the ability of bacterial cells to alert their kindred to increasing cell density (quorum sensing) have been described (17). Typically, bacterial cells produce a small extracellular signal molecu...

Transcriptional and Metabolomic Consequences of luxS Inactivation Reveal a Metabolic Rather than Quorum-Sensing Role for LuxS in Lactobacillus reuteri 100-23

“…The mutant strain also differed from the wild type in the ability to form a biofilm. Thicker biofilms were formed by the mutant in a bioreactor and on the epithelial surface of the mouse forestomach than by the wild type (15). The addition of purified AI-2 from Salmonella enterica serovar Typhimurium to the luxS mutant culture in a bioreactor did not restore wild-type biofilm characteristics (15).…”

“…Thicker biofilms were formed by the mutant in a bioreactor and on the epithelial surface of the mouse forestomach than by the wild type (15). The addition of purified AI-2 from Salmonella enterica serovar Typhimurium to the luxS mutant culture in a bioreactor did not restore wild-type biofilm characteristics (15). The luxS mutant strain had poor competitive ability with another Lactobacillus strain in the murine large bowel, indicating that LuxS was an important enzyme in gut ecology of L. reuteri 100-23 (15).…”

“…In previous work, a luxS mutant of Lactobacillus reuteri 100-23 did not grow differently from the wild type in rich growth medium but had a lower cellular ATP concentration (65%) than the wildtype strain (15). The mutant strain also differed from the wild type in the ability to form a biofilm.…”

“…The addition of purified AI-2 from Salmonella enterica serovar Typhimurium to the luxS mutant culture in a bioreactor did not restore wild-type biofilm characteristics (15). The luxS mutant strain had poor competitive ability with another Lactobacillus strain in the murine large bowel, indicating that LuxS was an important enzyme in gut ecology of L. reuteri 100-23 (15). We sought to determine the effect of the luxS mutation on the transcriptome and metabolome of L. reuteri 100-23 and to evaluate whether the impacts of the mutation were likely to be due to AI-2-mediated quorum sensing.…”

Cell-Cell Communication in Bacteria: United We Stand

“…S4: 7), provided new information on the potential immunomodulating and antimicrobial activities of Lactobacillus reuteri, a member of the probiotic and commensal microbiota in the mammalian oral cavity and gastrointestinal tract. These bacteria generally inhabit mixed-species biofilms where normal growth and development are governed by luxS-dependent signaling between the bacteria and the mammalian host (92). L. reuteri secretion of the broad-spectrum reuterin antibiotic and an undefined immunomodulin capable of inhibiting tumor necrosis factor alpha production is also luxS dependent.…”