Ecological changes in oral microcosm biofilm during maturation

Kim, Young Seok; Kang, Si Mook; Lee, Eun Song; Lee, Ji Hyun; Kim, Bo Ra; Kim, Baek Il

doi:10.1117/1.jbo.21.10.101409

Cited by 17 publications

(9 citation statements)

References 43 publications

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“…This is similar to our model but the biofilm was grown in narrow (100 μm) channels and not on composite or BE specimens. Furthermore, oral biofilms initiated from the saliva of a single donor have been grown anaerobically in basal medium mucin for up to 10 days and used to investigate changes in the microbiome by 16S rDNA gene amplicon sequencing of 10 species [14]. A complex in vitro biofilm initiated from the subgingival plaque of patients with periodontal disease was grown in trypticase soy broth with several reinoculation cycles to analyse the community structure by DNA-DNA checkerboard hybridisation covering seven species [38].…”

Section: Discussionmentioning

confidence: 99%

“…To each well, we added 250 µl of saliva and 50 µl cell-free filtrate of the corresponding saliva sample as a nutritional source. The microtiter plates were sealed and incubated under strictly anaerobic conditions at 37°C for 48 h. Anaerobic conditions were chosen because previous studies have established that most oral bacteria grow when such conditions are selected [13,14]. After 48 h, the in vitro specimens were collected and biofilm-like structures were verified for a few representative specimens by live/dead staining as above.…”

Section: Overall Study Designmentioning

confidence: 99%

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Deep sequencing of biofilm microbiomes on dental composite materials

Conrads

Wendt

Hetrodt

et al. 2019

Journal of Oral Microbiology

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Background: The microbiome on dental composites has not been studied in detail before. It has not been conclusively clarified whether restorative materials influence the oral microbiome. Methods: We used Illumina Miseq next-generation sequencing of the 16S V1-V2 region to compare the colonisation patterns of bovine enamel (BE) and the composite materials Grandio Flow (GF) and Grandio Blocs (GB) after 48 h in vivo in 14 volunteers. Applying a new method to maintain the oral microbiome ex vivo for 48 h also, we compared the microbiome on GF alone and with the new antimicrobial substance carolacton (GF+C). Results: All in vitro biofilm communities showed a higher diversity and richness than those grown in vivo but the very different atmospheric conditions must be considered. Contrary to expectations, there were only a few significant differences between BE and the composite materials GB and GF either in vivo or in vitro: Oribacterium, Peptostreptococcaceae [XI][G-1] and Streptococcus mutans were more prevalent and Megasphaera, Prevotella oulorum, Veillonella atypica, V. parvula, Gemella morbillorum, and Fusobacterium periodonticum were less prevalent on BE than on composites. In vivo, such preferences were only significant for Granulicatella adiacens (more prevalent on BE) and Fusobacterium nucleatum subsp. animalis (more prevalent on composites). On DNA sequence level, there were no significant differences between the biofilm communities on GF and GF+C. Conclusion: We found that the oral microbiome showed an increased richness when grown on various composites compared to BE in vitro, but otherwise changed only slightly independent of the in vivo or in vitro condition. Our new ex vivo biofilm model might be useful for pre-clinical testing of preventive strategies.

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Section: Discussionmentioning

confidence: 99%

Section: Overall Study Designmentioning

confidence: 99%

Deep sequencing of biofilm microbiomes on dental composite materials

Conrads

Wendt

Hetrodt

et al. 2019

Journal of Oral Microbiology

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“…However, they have focused on the outcome variables of RF plaque‐related to its effects on the oral tissue and have not considered the etiology underlying the biological characteristics of this plaque or the overall ecological changes. Indeed, there are few reports on the diversity and structure of fluorescent plaque microbiota in the real oral environment . In order to assess the clinical significance of RF plaque detected by QLF technology, it will be necessary to comprehensively understand the microbial characteristics of RF plaque and its potential relationship with the pathogenicity of oral disease.…”

Section: Introductionmentioning

confidence: 99%

Detection of dental plaque and its potential pathogenicity using quantitative light‐induced fluorescence

Lee

Jong

Kim

2019

Journal of Biophotonics

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Quantitative light‐induced fluorescence (QLF) technology can detect some dental plaque as red fluorescence. This in vivo study aimed to identify the microbial characteristics of red fluorescent (RF) dental plaque using 16S rRNA gene sequencing and evaluate the correlations between RF plaque and the clinical symptoms of dental diseases. Paired supragingival plaque samples collected from each 10 subjects and consisted of RF and non‐RF dental plaques as observed by QLF technology using a 405 nm blue light source for excitation. The characteristics of the bacterial communities in the RF and non‐RF plaque samples were compared by sequencing analysis. An increase in microbial diversity was observed in RF plaque compared with the non‐RF plaque. There were significant differences in the community compositions between the 2 types of dental plaque. Periodontopathic bacteria were significantly more abundant in the RF plaque than non‐RF plaque. The fluorescence intensity of RF plaque was significantly related to the proportion of the periodontopathic bacterial community and the presence of gingival inflammation. In conclusion, the plaque red fluorescence is associated with changes in the microbial composition and enrichment of periodontopathic pathogens, which suggests that RF plaque detected by QLF technology could be used as a risk indicator for gingival inflammation.

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“…The percentage of GC in the DNA is 40% (by either Tm method or Bd method) 19,20,21 . It has been abundantly present in the milk and milk production as well as also found in feces, saliva 22,23 , stomach, vagina, gastrointestinal tract, and oral cavity 24, 23 . This review aims to describe the pathogenicity and role of Lactobacillus in the formation of dental caries. Moreover, their ecology in the oral cavity and how to get prevent caries initiation.…”

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confidence: 99%

Untitled

2019

IJPSR

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Dental caries is an infective serious transmittable bacterial disease characterized by a multi-factorial pathology. Main players in the etiology of this disease are cariogenic bacteria, fermentable carbohydrates, a susceptible tooth, the host, and the time. It is still one of the most common disease worldwide. Biological factor such as cariogenic bacteria plays an important role in dental caries initiation, progression as well as tooth decay. Previous studies suggested that the main pathogenic bacteria are Streptococcus mutans and Lactobacillus. Consequently, Lactobacillus is considered the second most cariogenic bacteria of oral flora. It is not the caries initiator but plays an important role in caries progression. Most of the previous studies were quantitative, so till now, researchers not know about the specific species of Lactobacillus which are directly associated with dental caries progression. Some species-level identification studies suggested that the most common Lactobacillus species are L. gasseeri, L. fermentum, L. vaginalis and L. casei which were predominantly reported at the most of the oral sites, i.e. saliva, tongue, carious lesion, dental plaque, etc. and maybe plays the main role in caries progression. The present review spotlights the role, pathogenicity of Lactobacillus in dental caries and their ecology in the oral cavity.

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Ecological changes in oral microcosm biofilm during maturation

Cited by 17 publications

References 43 publications

Deep sequencing of biofilm microbiomes on dental composite materials

Deep sequencing of biofilm microbiomes on dental composite materials

Detection of dental plaque and its potential pathogenicity using quantitative light‐induced fluorescence

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