Ecological fitness cost associated with the AHAS Trp574Leu mutation in feralRaphanus sativus

Abstract

BACKGROUNDGene mutations endowing herbicide resistance may have negative pleiotropic effects on plant fitness. Quantifying these effects is critical for predicting the evolution of herbicide resistance and developing management strategies for herbicide-resistant weeds. This study reports the effects of the acetohydroxyacid synthase (AHAS) Trp574Leu mutation throughout the life cycle of the weed feral radish (Raphanus sativus L.).RESULTSResistant and susceptible biotypes responded differently to light and water treatments in relation to germination and emergence. Under light exposure, the resistant biotype showed higher germination and emergence, but no differences were found in seed dormancy, germination in darkness and emergence from buried seeds or pods. The resistant biotype showed delayed and reduced seedling emergence relative to the susceptible biotype under rainfed conditions, but these differences between the biotypes were not detected in irrigated soil. The biotypes showed similar relative growth rates and vegetative biomass. However, under wheat interference, resistant plants had 36–46% less total above-ground biomass, 26–47% less seeds per plant, and 36–53% less plant yield than susceptible ones, and these differences were more evident at higher plant density.CONCLUSIONThis study provides a better understanding of the ecological fitness cost associated with the AHAS Trp574Leu mutation in feral R. sativus. The fitness costs could reduce the frequency of the resistant allele in areas untreated with AHAS inhibiting herbicides.

Agricultural weeds: the contribution of domesticated species to the origin and evolution of feral weeds

“…Indeed, crop alleles with fitness costs can be introgressed and spread into weedy populations if selection is strong enough. 15,16 The expansion of international trade has increased the risk of biological invasions. Alien species are introduced intentionally (for example, for cultivation, breeding, and research) or accidentally (for example, as contaminants of traded goods) outside their native range through global commerce.…”

“…Cumulative evidence indicates that crop alleles underlying resistance to herbicides, 7–9 insects, 10–12 viruses, 13 and tolerance to drought stress 14 confer selective advantage in agricultural habitats and are expected to introgress into weedy populations. Indeed, crop alleles with fitness costs can be introgressed and spread into weedy populations if selection is strong enough 15,16 …”