Ecological Genetics of the Screwworm Fly,
Cochliomyia hominivorax
(Diptera: Calliphoridae) and Its Bearing on the Quality Control of Mass-reared Insects
1

Bush, Guy L.; Neck, Raymond W.

doi:10.1093/ee/5.5.821

Cited by 36 publications

(21 citation statements)

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“…Despite the very high numbers at which commercial A. swirskii populations are reared, genetic diversity can be low if the initial founding population is low (Mackauer, 1976;Bartlett, 1993). Genetic change in mass-reared populations has been described in many arthropods (Nunney, 2003), including experimental populations of D. melanogaster (Woodworth et al, 2002), but also in parasitoids reared for biological control (Guzm an-Larralde et al, 2014; Retamal et al, 2016;Bertin et al, 2017) and various insects reared for 'sterile insect technique' programmes since the 1980s (R€ ossler, 1975;Bush & Neck, 1976;Wong & Nakahara, 1978;Vargas & Carey, 1989). As the field environment is different from the mass-rearing conditions, adaptation to the rearing environment can have a negative impact on the fitness of mass-reared biocontrol agents in the field (Sørensen et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Effect of mass rearing on the genetic diversity of the predatory mite Amblyseius swirskii

Paspati

Ferguson

Verhulst

et al. 2019

Entomologia Exp Applicata

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Amblyseius swirskii Athias-Henriot (Acari: Phytoseiidae) is a predatory mite used to control whiteflies and thrips in protected crops. This biocontrol agent, originating from the Eastern Mediterranean region, has been mass-reared for commercial use since 2005 and is widely used in augmentative biocontrol programs. As a polyphagous predator, it has to cope with different biotic and abiotic factors. However, possible adaptation to mass rearing for production might be hindering its resilience and capacity for optimum performance in the field. In this study, we investigated the effect of long-term mass rearing on the genetic diversity of A. swirskii. We identified six microsatellite loci from wholegenome nanopore sequencing of A. swirskii and used these in a comparative analysis of the genetic diversity and differentiation in eight wild populations collected from Israel in 2017 and a commercially available population. Our results indicate that the commercial population is 2.59 less heterozygous than the wild A. swirskii. Furthermore, the commercial population has the highest genetic differentiation from all the natural populations, as indicated by higher pairwise F st values. Overall, we show that commercially reared A. swirskii have reduced genetic variation compared to their wild counterparts, which may reduce their performance when released to control pests in an integrated pest management (IPM) context.

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Section: Discussionmentioning

confidence: 99%

Effect of mass rearing on the genetic diversity of the predatory mite Amblyseius swirskii

Paspati

Ferguson

Verhulst

et al. 2019

Entomologia Exp Applicata

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“…These markers have expanded from traditional tools involving the cytological examination of mitotic and polytene chromosomes, genetic compatibility and isozyme analysis (Boyes, 1961;Boyes & VanBrink, 1965;Boyes and Shewell, 1975;Azeredo-Espin, 1982;Azeredo-Espin & Pavan, 1983;Bush & R.W. Neek., 1976 ;Whitten, 1980Whitten, , 1985Whitten, , 1986; LaChance & Whitten, 1986;LaChance et al, 1982) to include a vast array of molecular markers.…”

Section: Genetic Analysis Of Calliphoridae and Oestridae Species: A Hmentioning

confidence: 99%

Genetic Approaches for Studying Myiasis-causing Flies: Molecular Markers and Mitochondrial Genomics

Azeredo-Espin

Lessinger

2006

Genetica

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"Myiasis-causing flies" is a generic term that includes species from numerous dipteran families, mainly Calliphoridae and Oestridae, of which blowflies, screwworm flies and botflies are among the most important. This group of flies is characterized by the ability of their larvae to develop in animal flesh. When the host is a live vertebrate, such parasitism by dipterous larvae is known as primary myiasis. Myiasis-causing flies can be classified as saprophagous (free-living species), facultative or obligate parasites. Many of these flies are of great medical and veterinary importance in Brazil because of their role as key livestock insect-pests and vectors of pathogens, in addition to being considered important legal evidence in forensic entomology. The characterization of myiasis-causing flies using molecular markers to study mtDNA (by RFLP) and nuclear DNA (by RAPD and microsatellite) has been used to identify the evolutionary mechanisms responsible for specific patterns of genetic variability. These approaches have been successfully used to analyze the population structures of the New World screwworm fly Cochliomyia hominivorax and the botfly Dermatobia hominis. In this review, various aspects of the organization, evolution and potential applications of the mitochondrial genome of myiasis-causing flies in Brazil, and the analysis of nuclear markers in genetic studies of populations, are discussed.

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“…Bush & Neck (1976) identified five variable enzyme loci in laboratory colonies of screwworm, but they did not examine any field populations . Richardson et al .…”

mentioning

confidence: 99%

Population Genetics and Gene Variation in Primary and Secondary Screwworm (Diptera: Calliphoridae)

Taylor¹,

Peterson²

1994

Annals of the Entomological Society of America

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Allozyme variation in screwworm, Cochliomyia hominivorax (Coquerel), and secondary screwworm, C . macellaria (F.), populations from northwest Costa Rica was examined . Variability was observed in 11 of 13 enzyme loci and the frequency of the most common allele was <0 .95 for 5 loci in screwworm . In secondary screwworm, 12 of 13 loci were variable and the frequency of the most common allele was <0 .95 for 6 loci . Expected heterozygosities were 0 .149 and 0 .160 for screwworm and secondary screwworm, respectively . Goodness-of-fit statistics for Hardy-Weinberg equilibrium and Wrights F statistics indicated that both species are panmictic with no evidence of population substructuring. Nei ' s genetic distances were 0.000-0 .001 for intraspecific comparisons and 0 .899-0 .916 for interspecific comparisons . The data indicate a high level of gene flow between populations within each species.

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Ecological Genetics of the Screwworm Fly, Cochliomyia hominivorax (Diptera: Calliphoridae) and Its Bearing on the Quality Control of Mass-reared Insects 1

Cited by 36 publications

References 0 publications

Effect of mass rearing on the genetic diversity of the predatory mite Amblyseius swirskii

Effect of mass rearing on the genetic diversity of the predatory mite Amblyseius swirskii

Genetic Approaches for Studying Myiasis-causing Flies: Molecular Markers and Mitochondrial Genomics

Population Genetics and Gene Variation in Primary and Secondary Screwworm (Diptera: Calliphoridae)

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