Macro seaweeds were collected seasonally with a beam trawl from seven fixed stations in infralittoral of Gulf of Izmir to study spatiotemporal distribution of macrobenthic flora and their ecology during 2009-2010 years. A total of 6 floral species were found during the study. They were composed of an endemic marine phanerogam, Posidonia oceanica, and 5 seaweeds. The marine plants were distributed almost separately in different sectors of the gulf. Maximum average biomass was estimated for Codium bursa, followed by Ulva lactuca, Codium vermilara and dead leaves of Posidonia oceanica. Codium vermilara was found only in the inner gulf (sector), three algal seaweeds were found in the middle gulf, and Codium bursa and Posidonia oceanica in the outer gulf. A significant factor was the sector because each sector had different segmentation of the bottom depths. There was no seasonal difference even though each species contributed to the total biomass in different season. Flora assemblages were oriented with sectors of the gulf, which explained with two components. The first component was as follows: near-bottom water density followed by sea surface density was then correlated with the macrophyte biomass. Temperature of sea surface and near-bottom waters was negatively correlated with flora assemblages formed. The second component was followed by that pH of sea surface and near-bottom waters negatively correlated with the macro-seaweed communities which was positively and slightly correlated with seafloor depth and dissolved oxygen of near-bottom waters.