Ecological life strategies of microbes in response to antibiotics as a driving factor in soils

Sharma, Udita; Rawat, Deepak; Mukherjee, Paromita; Farooqi, Furqan; Mishra, Vandana; Sharma, Raghav

doi:10.1016/j.scitotenv.2022.158791

Cited by 17 publications

(4 citation statements)

References 94 publications

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“…Since the discovery of penicillin in 1928, antibiotics have been fully integrated into people's daily lives 34 . Antibiotics are commonly used in human and veterinary medicine 35 , and an increasing number of antibiotics are being detected in aquatic environments 36 .…”

Section: Resultsmentioning

confidence: 99%

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Zhao,

Li,

2024

Sci Rep

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Previous studies reported microplastics (MPs), antibiotics, and antibiotic resistance genes (ARGs) in wastewater treatment plants (WWTPs). There is still a lack of research progress on the origin, fate, impact and hazards of MPs and ARGs in WWTPs. This paper fills a gap in this regard. In our search, we used “microplastics”, “antibiotic resistance genes”, and “wastewater treatment plant” as topic terms in Web of Science, checking the returned results for relevance by examining paper titles and abstracts. This study mainly explores the following points: (1) the origins and fate of MPs, antibiotics and ARGs in WWTPs; (2) the mechanisms of action of MPs, antibiotics and ARGs in sludge biochemical pools; (3) the impacts of MPs in WWTPs and the spread of ARGs; (4) and the harm inflicted by MPs and ARGs on the environment and human body. Contaminants in sewage sludge such as MPs, ARGs, and antibiotic-resistant bacteria enter the soil and water. Contaminants can travel through the food chain and thus reach humans, leading to increased illness, hospitalization, and even mortality. This study will enhance our understanding of the mechanisms of action among MPs, antibiotics, ARGs, and the harm they inflict on the human body.

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Section: Resultsmentioning

confidence: 99%

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Zhao,

Li,

2024

Sci Rep

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“…On the other hand, carbohydrate degradation was prominent for environmentally isolated strains and lower virulence. In recent years, life history traits analysis has been applied to analyze environmental and antibiotic-resistant microorganisms (39, 40). In particular, stress-response such as resistance to antibiotics are known to impose a burden on microbial physiology with consequences for growth and resource acquisition (41, 42).…”

Section: Discussionmentioning

confidence: 99%

Variation in the response to antibiotics and life-history across the majorPseudomonas aeruginosaclone type (mPact) panel

Tueffers,

Batra,

Zimmermann

et al. 2024

Preprint

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Pseudomonas aeruginosais a ubiquitous, opportunistic human pathogen. Since it often expresses multidrug resistance, it is ranked by the World Health Organization among the top 3 high priority pathogens, for which new treatment options are urgently required. An evaluation of new treatments is usually performed experimentally with one of the canonical laboratory strains (e.g., PAO1 or PA14). However, these two strains are unlikely representative of the strains infecting patients, because they have adapted to laboratory conditions and do not capture the enormous genomic diversity of the species. Here, we characterized the majorP. aeruginosaclone type (mPact) panel. This panel consists of 20 strains, which reflect the genomic diversity of the species, cover all major clone types, and have both patient and environmental origins. We found significant strain variation in distinct responses towards antibiotics and general growth characteristics. Only few of the measured traits are related, and if so, only for specific antibiotics. Moreover, high levels of resistance were only identified for clinical mPact isolates and could be linked to known AMR (antimicrobial resistance) genes in the sequenced genomes. One strain also produced highly unstable AMR, indicating an evolutionary cost to resistance expression. By linking isolation source, growth, and virulence to life history traits, we further identified specific adaptive strategies for individual mPact strains towards either host processes or degradation pathways. Overall, the mPact panel provides a reasonably sized set of distinct strains, enabling in-depth analysis of new treatment designs or evolutionary dynamics in consideration of the species' genomic diversity.

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“…On the other hand, carbohydrate degradation was prominent for environmentally isolated strains and lower virulence. In recent years, life history trait analysis has been applied to analyze environmental and antibiotic-resistant microorganisms ( 52 , 53 ). In particular, stress-response such as resistance to antibiotics are known to impose a burden on microbial physiology with consequences for growth and resource acquisition ( 54 , 55 ).…”

Section: Discussionmentioning

confidence: 99%

Variation in the response to antibiotics and life-history across the major Pseudomonas aeruginosa clone type (mPact) panel

Tueffers,

Batra,

Zimmermann

et al. 2024

Microbiol Spectr

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Pseudomonas aeruginosa is a ubiquitous, opportunistic human pathogen. Since it often expresses multidrug resistance, new treatment options are urgently required. Such new treatments are usually assessed with one of the canonical laboratory strains, PAO1 or PA14. However, these two strains are unlikely representative of the strains infecting patients, because they have adapted to laboratory conditions and do not capture the enormous genomic diversity of the species. Here, we characterized the major P. aeruginosa clone type (mPact) panel. This panel consists of 20 strains, which reflect the species’ genomic diversity, cover all major clone types, and have both patient and environmental origins. We found significant strain variation in distinct responses toward antibiotics and general growth characteristics. Only few of the measured traits are related, suggesting independent trait optimization across strains. High resistance levels were only identified for clinical mPact isolates and could be linked to known antimicrobial resistance (AMR) genes. One strain, H01, produced highly unstable AMR combined with reduced growth under drug-free conditions, indicating an evolutionary cost to resistance. The expression of microcolonies was common among strains, especially for strain H15, which also showed reduced growth, possibly indicating another type of evolutionary trade-off. By linking isolation source, growth, and virulence to life history traits, we further identified specific adaptive strategies for individual mPact strains toward either host processes or degradation pathways. Overall, the mPact panel provides a reasonably sized set of distinct strains, enabling in-depth analysis of new treatment designs or evolutionary dynamics in consideration of the species’ genomic diversity. IMPORTANCE New treatment strategies are urgently needed for high-risk pathogens such as the opportunistic and often multidrug-resistant pathogen Pseudomonas aeruginosa . Here, we characterize the major P. aeruginosa clone type (mPact) panel. It consists of 20 strains with different origins that cover the major clone types of the species as well as its genomic diversity. This mPact panel shows significant variation in (i) resistance against distinct antibiotics, including several last resort antibiotics; (ii) related traits associated with the response to antibiotics; and (iii) general growth characteristics. We further developed a novel approach that integrates information on resistance, growth, virulence, and life-history characteristics, allowing us to demonstrate the presence of distinct adaptive strategies of the strains that focus either on host interaction or resource processing. In conclusion, the mPact panel provides a manageable number of representative strains for this important pathogen for further in-depth analyses of treatment options and evolutionary dynamics.

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Ecological life strategies of microbes in response to antibiotics as a driving factor in soils

Cited by 17 publications

References 94 publications

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Research progress on the origin, fate, impacts and harm of microplastics and antibiotic resistance genes in wastewater treatment plants

Variation in the response to antibiotics and life-history across the majorPseudomonas aeruginosaclone type (mPact) panel

Variation in the response to antibiotics and life-history across the major Pseudomonas aeruginosa clone type (mPact) panel

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