Ecological occurrence and plant regeneration of embryoid of the endangered and endemic plantDysosma versipellisin China

Abstract

In this study, the effective callus culture, somatic embryogenesis, and plant 9 regeneration system of Dysosma versipellis, which is an endangered and endemic plant in China, 10 were established under specific culture conditions. Using the D. versipellis leaves, petioles, and 11 roots as explants, DPS software orthogonal design method and SPSS Duncan's multiple range test 12 were used to investigate their effects of D. versipellis on callus formation, embryoid induction,13 and plant regeneration by adding different phytohormones. Results showed that leaves and 14 petioles were the most suitable materials in inducing callus. The effect of phytohormone on callus 15 formation followed the order of 2,4-dichlorophenoxyacetic acid (2,4-D)>thidiazuron (TDZ)> 16 kinetin>naphthylacetic acid (NAA)>2-ip. The best medium for callus formation was MS+2,4-D 1 17 mg/L+NAA 0.05 mg/L+TDZ 0.5 mg/L+2-ip 1 mg/L. The optimal medium to induce the 18 formation of granular callus embryoid was MS+0.5 mg/L 6-BA+0.1 mg/L NAA, and the 19 induction rate was 71.33%. The embryoid rooting and plant regeneration medium was MS+0.5 20 mg/L IBA+0.5 mg/L GA3. The optimal medium formula obtained in this study was suitable for 21 the rapid induction of callus, embryoid, and plant regeneration of D. versipellis under in vitro 22culture conditions. Further study on the action mechanism, signal regulation mechanism, and 23 artificial seed production of fungal elicitors affecting the accumulation of podophyllotoxin is 24 important. 25