Water is the ultimate and mostly used media during textile materials processing, especially in colouration. This study investigated the possibilities of using seawater for cotton and polyester fabrics dyeing. Single jersey fabrics made of 100 percent cotton and polyester were dyed using a standard recipe and two separate water source as dyeing mediums. It has been focused on the assessment of colour fastness to wash, perspiration, saliva, rubbing, water, light and colour difference value due to compare the efficiency of dyeing media. The results revealed that the cotton fabric dyed with seawater showed lighter shade than that of ground water sample. But for polyester fabric darker shade was obtained compared to ground water. The cotton sample dyed with sea water carried about 15% higher colour strength than ground water dyed sample but for polyester it was very negligible, only 3%. Moreover, the results of colour fastness to wash, perspiration, saliva, rubbing, water and light for seawater dyed samples of cotton and polyester were shown satisfactory outcomes having the grading of 4–5 in most of the cases. This exploration established that commercial dyeing processes were robust and can be practically transferable into the seawater medium for cotton and polyester fabrics.