Ecological risk of 17α-methyltestosterone contaminated water discharged from a full water recirculating earthen masculinization pond

Thanasupsin, Sudtida Pliankarom; Chheang, Lita; Math, Chim

doi:10.1080/10807039.2021.1871845

Cited by 15 publications

(6 citation statements)

References 39 publications

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“…The circulating water system can also accumulate steroid levels and produce a cumulative masculinizing effect. This is in accordance with previous research, where increasing the dose of steroid hormone was proven to be able to produce male individuals in intensive culture ponds (with strong aeration) compared to culture ponds with water flow aeration systems (Thanasupsin et al, 2021).…”

confidence: 93%

Effect of Dosages and Temperatures on Simalungun Red Ginger (Zingiber officinale var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (Danio rerio) as a Model Fish

Febriani

Widodo²,

Faqih³

2023

JIPK

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Highlight Research Successive administration of Ginger ethanol extract to male individuals was shown to be able to produce a significant increase in sperm count, motility, viability and morphology Ethanol extract of ginger has androgenic activity which is proven to be able to increase the concentration of testosterone in serum, which functions to control the process of spermatogenesis, maintain Sertoli cells and play a role in determining the quality of spermatozoa in male organisms Giving ginger ethanol extract for 30 consecutive days to male individuals showed significant results so that ginger extract was thought to have the potential to improve male characteristics Ginger extract is potentially useful in improving healthy sperm characteristics and management of male infertility especially in those with low sperm counts Abstract Ornamental fish are known to be easily cultivated in aquarium or pond. Many people prefer male (MF) over female fish (FF) due to their aesthetic value and productivity which lead to better pricing. The aim of this study was to determine the effect of Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG) on masculinization. This study used dipping method which was carried out using Simalungun Red Ginger (Zingiber officinale var. Rubrum) (SRG). The temperature treatments used in this study were 28°C and 32°C. For this research, an experimental study with a Completely Randomized Factorial Design (CRFD) method that had two independent variables such as different dosages and temperatures was used. From five dosages (0 mg/L; 5 mg/L; 10 mg/L; 15 mg/L; 20 mg/L) and three temperature treatments (ambient temperature (about 25°C; 28°C; 32°C), it was found that the highest male percentage of primary sexuality (88.33%), secondary sexuality (85%), and highest total testosterone levels (TL) (1.986 ng/L) were at the treatments with the highest dosages and temperature, while for the highest survival rate were at almost in all B treatments (5 mg/L) and b levels (28°C), in which the survival rate was 100%. For all of these results above, the results were significant.

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confidence: 93%

Effect of Dosages and Temperatures on Simalungun Red Ginger (Zingiber officinale var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (Danio rerio) as a Model Fish

Febriani

Widodo²,

Faqih³

2023

JIPK

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“…However, fish treated with synthetic MT had altered enzymatic metabolic pathways [ 30 ], as well as a substantial increase in plasma creatine phosphokinase activity and DNA fragmentation in the liver tissue [ 31 ]. The dynamics of the ecosystem may be affected if tilapia hatcheries discharge steroids into the aquatic environment [ 32 ]. Furthermore, in certain natural populations, this may result in more males than females, leading to long-term reproductive failure [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Masculinization of Red Tilapia (Oreochromis spp.) Using 17α-Methyltestosterone-Loaded Alkyl Polyglucosides Integrated into Nanostructured Lipid Carriers

Yostawonkul

Kitiyodom

Supchukun

et al. 2023

Animals

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The aim of the present study was to optimize a masculinization platform for the production of all-male red tilapia fry by oral administration of 30 and 60 ppm of MT and alkyl polyglucoside nanostructured lipid carriers (APG-NLC) loaded with MT, respectively, for 14 and 21 days. The characterization, encapsulation efficiency and release kinetics of MT in lipid-based nanoparticles were assessed in vitro. The results showed that the MT-loaded nanoparticles were spherical, ranging from 80 to 125 nm in size, and had a negative charge with a narrow particle distribution. The APG-NLC loaded with MT provided higher physical stability and encapsulation efficacy than the NLC. The release rate constants of MT from MT-NLC and MT-APG-NLC were higher than those of free MT, which is insoluble in aqueous media. There was no significant difference in survival between the fish administered MT or the those fed orally with MT-APG-NLC fish. According to the logistic regression analysis, the sex reversal efficacy of MT-APG-NLC (30 ppm) and MT (60 ppm), resulted in significantly higher numbers of males after 21 days of treatment compared with the controls. The production cost of MT-APG-NLC (30 ppm) after 21 days of treatment was reduced by 32.9% compared with the conventional MT treatment group (60 ppm). In all the treatments, the length–weight relationship (LWR) showed negatively allomeric growth behavior (b < 3), with a relative condition factor (Kn) of more than 1. Therefore, MT-APG-NLC (30 ppm) would seem to be a promising, cost-effective way to reduce the dose of MT used for the masculinization of farmed red tilapia.

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“…The fourth compound investigated is a testosterone analog belonging to this group, namely methyltestosterone, i.e., 17 α -methyltestosterone (MTTT), which bears a methyl group at the carbon atom C-17. MTTT has been broadly used in aquafarming and more recently concerns have been raised about its ecological risk [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

Conformations of Steroid Hormones: Infrared and Vibrational Circular Dichroism Spectroscopy

et al. 2023

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Steroid hormone molecules may exhibit very different functionalities based on the associated functional groups and their 3D arrangements in space, i.e., absolute configurations and conformations. Infrared (IR) and vibrational circular dichroism (VCD) spectra of four different steroid hormones, namely dehydroepiandrosterone (DHEA), 17α-methyltestosterone (MTTT), (16α,17)-epoxyprogesterone (Epoxy-P4), and dehydroepiandrosterone acetate (AcO-DHEA), were measured in deuterated dimethyl sulfoxide and some also in carbon tetrachloride. Extensive conformational searches were carried out using the recent developed conformer-rotamer ensemble sampling tool (CREST) which also accounts for solvent effects using an implicit solvation model. All the CREST conformational candidates were then reoptimized at the B3LYP-D3BJ/def2-TZVPD with the PCM of solvent. The good agreements between the experimental IR and VCD spectra and the theoretical simulations provide a conclusive information about their conformational distribution and absolute configurations. The experimental and theoretical IR and VCD spectra of AcO-DHEA in the carbonyl and alkene stretching region showed some discrepancies, and the possible causes related to solvent effects, large amplitude motions and levels of theory used in the modelling were explored in detail. As part of the investigation, additional calculations at the B3LYP-D3BJ/6-31++G (2d,p) and B3LYP-D3BJ/cc-pVTZ levels, as well as some ‘mixed’ calculations with the double-hybrid functional B2PLYP-D3 were also carried out. The results indicate that the double-hybrid functional is important for predicting the correct IR band pattern in the carbonyl and alkene stretching region.

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Ecological risk of 17α-methyltestosterone contaminated water discharged from a full water recirculating earthen masculinization pond

Cited by 15 publications

References 39 publications

Effect of Dosages and Temperatures on Simalungun Red Ginger (<I>Zingiber officinale</I> var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (<I>Danio rerio</I>) as a Model Fish

Effect of Dosages and Temperatures on Simalungun Red Ginger (<I>Zingiber officinale</I> var. Rubrum) Ethanol Extract Dipping on Masculinization of Zebra Fish (<I>Danio rerio</I>) as a Model Fish

Masculinization of Red Tilapia (Oreochromis spp.) Using 17α-Methyltestosterone-Loaded Alkyl Polyglucosides Integrated into Nanostructured Lipid Carriers

Conformations of Steroid Hormones: Infrared and Vibrational Circular Dichroism Spectroscopy

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