Ecology, Physiology and Taxonomy Studies on a New Taxon of Haloanaerobiaceae, Haloincola saccharolytica gen. nov., sp. nov.

Zhilina, T. N.; Zavarzin, G. A.; Булыгина, Е. С.; Kevbrin, V. V.; Осипов, Г. А.; Chumakov, Konstantin

doi:10.1016/s0723-2020(11)80101-3

Cited by 64 publications

(26 citation statements)

References 18 publications

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“…Haloanaerobium acetoethylicum [formerly Halobacteroides acetoethylicus (Rainey et al, 1995 ;Rengpipat et al, 1988) (Rainey et al, 1995;Zhilina et al, 1992)] subsp. saccharolyticum (= DSM 6643T) was kindly provided by Dr T. N. Zhilina.…”

Section: Strainsmentioning

confidence: 99%

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Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine

Bhupathiraju

McInerney

Woese

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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Three strains, designated VS-75IT, VS-511 and VS-732, of a strictly anaerobic, moderately halophilic, Gram-negative, rod-shaped bacterium were isolated from a highly saline (15-20%) brine from an oil reservoir in central Oklahoma, USA. The optimal concentration of NaCl for growth of these three strains was 2 M (12%), and the strains also grew in the presence of an additional 1 M MgCI,. The strains were mesophilic and grew a t a pH range of 6-8. Carbohydrates used by all three strains included glucose, fructose, arabinose, galactose, maltose, mannose, cellobiose, sucrose and inulin. Glucose fermentation products included ethanol, acetate, H, and CO, , with formate produced by two of the three strains. Differences were noted among strains in the optimal temperature and pH for growth, the maximum and minimum NaCl concentration that supported growth, substrate utilization and cellular fatty acid composition. Despite the phenotypic differences among the three strains, analysis of the 165 rRNA gene sequences and DNA-DNA hybridizations showed that these three strains were members of the same genospecies which belonged to the genus Haloanaerobium. The phenotypic and genotypic characteristics of strains VS-75IT, VS-511 and VS-732 are different from those of previously described species of Haloanaerobium. It is proposed that strain VS-75IT (ATCC 7001033 be established as the type strain of a new species, Haloanaerobium kushneri.

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Section: Strainsmentioning

confidence: 99%

“…saccharolyticum (formerly Haloincola saccharolyticum subsp. saccharolyticum) (Rainey et al, 1995 ;Tourova et al, 1995;Zhilina et al, 1992), Haloanaerobium saccharolyticum subsp. senegalensis (formerly Haloincola saccharolyticum subsp.…”

Section: Introductionmentioning

confidence: 99%

Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine

Bhupathiraju

McInerney

Woese

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

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“…Strain H200T can be differentiated from Haloanaerobacter chitinovorans (15) on the basis of its metabolic properties (production of ethanol) and cell size. In contrast to Haloincola saccharolytica (5,35), strain H200T produces ethanol and has a broader salt tolerance and optimum salt concentration range. Halobacteroides species (24,26,33), Haloanaerobium species (2,30,32), and strain H200T all produce ethanol, acetate, CO,, and H, as end products during glucose fermentation.…”

Section: Discussionmentioning

confidence: 97%

“…Strain H200T is not a member of the genus Halothermothrix. Strain H200T can be easily differentiated from members of other genera, including the genera Sporohalobacter (21), Haloanaerobacter (15), Halocella (28), and Haloincola (5,35), on the basis of its phenotypic and phylogenetic characteristics. Phenotypically, strain H200T differs from Sporohalobacter species (21) in its cell morphology, salt tolerance, pattern of glucose fermentation, and lack of sporulation.…”

Section: Discussionmentioning

confidence: 99%

Haloanaerobium lacusroseus sp. nov., an Extremely Halophilic Fermentative Bacterium from the Sediments of a Hypersaline Lake

Cayol¹,

Ollivier²,

Patel

et al. 1995

International Journal of Systematic Bacteriology

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A new extremely halophilic chemoorganotrophic bacterium (strain H200T [T = type strain]) was isolated from the hypersaline sediments of Retba Lake in Senegal. This organism was a sluggishly motile, rod-shaped, non-spore-forming, gram-negative, obligate anaerobe that grew optimally at 40°C in the presence of 180 to 200 g of NaCl per liter. The DNA base composition was 32 mol% guanine plus cytosine. The fermentation products from glucose were ethanol, acetate, H,, and CO,. Yeast extract was required for growth. The fermentable substrates included D-fructose, galactose, D-xylose, cellobiose, lactose, maltose, sucrose, starch, D-mannitol, glycerol, and Casamino Acids. On the basis of the results of a 16s rRNA sequence analysis, strain H200T was found to be related to Haloanaerobium species. The 16s rRNA sequence of strain H200T differed from the sequences of the three previously described Haloanaerobium species, and strain H200T also differed from these organisms in its NaCl range for growth (60 to 340 g/liter); strain H200T grew in the presence of the highest NaCl concentration recorded for any halophilic anaerobic organism, including the three previously described Haloanaerobium species. We propose that strain H200T (= DSM 10165) belongs to a new Haloanaerobium species, Haloanaerobium lacusroseus.Scientific interest in extremophilic anaerobic microorganisms has recently increased because of the possible biotechnological use of enzymes and molecules from such organisms (17). Among the extremophilic anaerobic bacteria, less attention has been paid to extreme halophiles (organisms that grow most rapidly in the presence of 200 to 300 g of NaCl per liter) than to hyperthermophiles.Hypersaline ecosystems (inland lakes, marine salterns) exhibit great variability in ionic composition, total salt concentration, and pH and are inhabited by aerobic and anaerobic microfloras (19). The strictly anaerobic halophiles that have been isolated from hypersaline ecosystems include sulfate-reducing bacteria, methanogenic bacteria, phototrophic bacteria, and fermentative bacteria (19, 21). Of the 14 species of fermentative halophilic anaerobes belonging to the family Haloanaerobiaceae that have been described (21, 23), only the following 3 are extreme halophiles that exhibit optimum growth in the presence of approximately 200 g of NaCl per liter but do not at more than 300 g of NaCl per liter: Halobacteroides lacunaris, an organism obtained from hypersaline Lake Chokrak on the Kerch Peninsula (33); Haloanaerobacter chitinovorans, an organism obtained from a southern California solar saltern (15); and Acetohalbbiurn arabaticum, an organism obtained from Lake Sivash (34).As very little attention has been paid to the extremely halophilic fermentative anaerobic bacteria, we initiated work to isolate such bacteria from hypersaline environments. In this paper we describe the isolation from the sediment of a hyper- saline lake of an extremely halo hilic chemoorganotrophic anaerobic bacterium, strain H200 (T = type strain), which grows in the ...

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“…Halocella cellulolytica ferments glucose to acetate, ethanol, lactate, H,, and CO, (31). Haloincola saccharolytica (2,40) and Acetohalobium arabaticum (39) exhibit a homoacetogenic pathway of glucose metabolism.…”

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confidence: 99%

Haloanaerobium alcaliphilum sp. nov., an Anaerobic Moderate Halophile from the Sediments of Great Salt Lake, Utah

Tsai

Garcia

Patel

et al. 1995

International Journal of Systematic Bacteriology

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A strictly anaerobic, moderately halophilic, gram-negative, rod-shaped bacterium was isolated from Great Salt Lake, Utah, sediments and designated GSLST (T = type strain). Strain GSLST grew optimally at pH 6.7 to 7.0 but had a very broad pH range for growth (pH 5.8 to 10.0). The optimum temperature for growth was 37"C, and no growth occurred at 15 or 55°C. The optimum salt concentration for growth was 10%. Strain GSLST required yeast extract and Trypticase peptone to ferment carbohydrates, pyruvate, and glycine betaine. Strain GSLST was resistant to penicillin, D-cycloserine, tetracycline, and streptomycin. The G+C content of this isolate was 31 mol%. The fermentation products from glucose utilization were acetate, butyrate, lactate, CO,, and H,, and in addition strain GSLST fermented glycine betaine to acetate and trimethylamine. All of these traits distinguish this organism from all previously described halophilic anaerobes. The 16s rRNA gene sequence of strain GSLST was found to be similar to, but also significantly different from, the 16s rRNA sequences of Haloanuerobium sahugo and Haloanaerobium praevalens. Therefore, strain GSLST ( = DSM 827ST) is described as a new species, Haloanaerobium alcaliphilum.

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Ecology, Physiology and Taxonomy Studies on a New Taxon of Haloanaerobiaceae, Haloincola saccharolytica gen. nov., sp. nov.

Cited by 64 publications

References 18 publications

Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine

Haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine

Haloanaerobium lacusroseus sp. nov., an Extremely Halophilic Fermentative Bacterium from the Sediments of a Hypersaline Lake

Haloanaerobium alcaliphilum sp. nov., an Anaerobic Moderate Halophile from the Sediments of Great Salt Lake, Utah

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