The current study was conducted at Experimental Farm of Department of Floriculture and Landscape Architecture, Dr. YS Parmar UHF, Nauni, Solan, H.P. during the year 2019-2020 to determine the ideal Jeevamrit dosage for enhancing the physico-chemical and microbiological characteristics of soil for irisAt intervals of 15 days, sixteen applications of jeevamrit were applied as a foliar spray and drench. Three replications of the field experiment were set up using Randomized Block Design (RBD). The study showed that treatment T16, which involved both 7.5% Jeevamrit drenching and 15.0% Jeevamrit foliar application, had the most positive impact on soil quality. This treatment resulted in the highest organic carbon level (1.48), nitrogen content (362.59 kg/ha), phosphorus content (137.48 kg/ha), potassium content (267.85 kg/ha), and a high count of viable bacteria (75.63 ×106 cfu/g soil), viable fungi count (28.57 ×104 cfu/g soil), and viable actinomycetes count (45.90 ×103 cfu/g soil). Additionally, this treatment had the lowest pH and EC levels.