Studies related to the attainment of new sources of enzymes have increased significantly, especially works regarding the glycosidase production, such as cellulases, xylanases, among others. In this context, this work verified the cellulase production from fungi associated with cassava stems. It also analyzed the efficiency to apply enriched extracts to this class of enzymes in the hydrolysis of sugar cane bagasse treated with the sec-butylammonium acetate ionic liquid (IL). For this, the cellulase production by 14 fungi isolated from cassava stems was evaluated, verifying a higher production by two fungi: the Annulohypoxylon stygium fungus (CA14), which showed a concentration of fermentable sugars during the microcrystalline cellulose hydrolysis of 0.83 ± 0.01 mg.mL-1 and the Phanerochaetaceae sp. fungus (CA17), with a concentration of 0.46 ± 0.03 mg.mL-1. Afterwards, the influence of time in the growth of these fungi (CA14 and CA17) in a liquid medium was verified. It was possible to determine an ideal growth period of 13 days for CA14 (enzymatic activity of 0.212 ± 0.002 FPU.mL-1) and 14 days for CA17 (0.201 ± 0.002 FPU.mL-1). Having verified the highest cellulase production by CA14, the ideal growth pH was also evaluated for this fungus (pH 4, activity of 0.427 ± 0.003 FPU.mL-1). Following the determination of the ideal growth condition for the CA14 fungus, a new growth process was realized. The enzymatic extract obtained was applied to the hydrolysis process of the sugar cane bagasse treated with the sec-butylammonium acetate IL (the best ionic liquid when compared to others evaluatedimidazolium acetate, imidazolium lactate, and secbutylammonium lactate). Finally, a prepurification of the enzymatic extract was carried out using an ammonium sulphate precipitation with saturations of 25, 50, and 75 %. All fractions were analyzed regarding the hydrolysis of the sugar cane bagasse treated with the selected IL, verifying a higher cellulase concentration in the fraction obtained after 50 % of saturation (0.430 ± 0.002 FPU.mL-1).