Abstract. The efficient and economic organization of the railway fuel supply and good diesel locomotive fuel supply management is very significant to ensure the railway transport safety, punctuality, good service, transportation and production costs reduced, and it also improves the economic efficiency. This study comes from the reality of China's rail fuel supply business processes. It established the rail fuel inventory-transportation integrated optimization model whose goal is the total cost of the minimum and uses iterative genetic algorithm to solve it. Then we get the final optimal order quantity, total cost and transport path. The result provides a reference for the optimization of train fuel supply chain.