The EU low-carbon
economy aims to reduce the level of
CO
2
emission in the EU to 80% by 2050. High efforts are
required to
achieve this goal, where successful CCU (Carbon Capture and Utilization)
technologies will have a high impact. Biocatalysts offer a greener
alternative to chemical catalysts for the development of CCU strategies
since biocatalysis conforms 10 of the 12 principles of green chemistry.
In this study, a multienzymatic system, based on alcohol dehydrogenase
(ADH), pyruvate decarboxylase (PDC), and lactate dehydrogenase (LDH),
that converts CO
2
and ethanol into lactic acid leading
to a 100% atom economy was studied. The system allows cofactor regeneration,
thus reducing the process cost. Through reaction media engineering
and enzyme ratio study, the performance of the system was able to
produce up to 250 μM of lactic acid under the best conditions
using 100% CO
2
, corresponding to the highest concentration
of lactic acid obtained up to date using this multienzymatic approach.
For the first time, the feasibility of the system to be applied under
a real industrial environment has been tested using synthetic gas
mimicking real blast furnace off-gases composition from the iron and
steel industry. Under these conditions, the system was also capable
of producing lactic acid, reaching 62 μM.