Economic photoprotection in photosystem II that retains a complete light-harvesting system with slow energy traps

Belgio, Erica; Kapitonova, Ekaterina; Chmeliov, Jevgenij; Duffy, Christopher D. P.; Ungerer, Petra; Valkūnas, Leonas; Ruban, Alexander V.

doi:10.1038/ncomms5433

Cited by 84 publications

(64 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The PSII as the heart of the photosynthetic process is highly sensitive to the environmental stresses. Its activity could be determined with one non-invasive time-resolved fluorescence measurement, which could measure the polyphasic rise with the basic steps of OJIP [43,[48][49][50][51][52][53][54]. Several studies have shown that the chlorophyll a transient has become one of the most popular tools to estimate stress-induced changes in photosynthetic performance.…”

Section: Discussionmentioning

confidence: 99%

Night Light-Adaptation Strategies for Photosynthetic Apparatus in Yellow-Poplar (Liriodendron tulipifera L.) Exposed to Artificial Night Lighting

Kwak

Cheng

et al. 2018

Forests

View full text Add to dashboard Cite

Abstract:Plants can undergo external fluctuations in the natural light and dark cycle. The photosynthetic apparatus needs to operate in an appropriate manner to fluctuating environmental factors, especially in light. Yellow-poplar seedlings were exposed to nighttime artificial high-pressure sodium (HPS) lighting to evaluate night light-adaptation strategies for photosynthetic apparatus fitness relative to pigment contents, photosystem II photochemistry, photosynthetic parameters, histochemical analysis of reactive oxygen species, and plant biomass. As a result, seedlings exhibited dynamic changes including the enhancement of accessory pigments, the reduction of photosystem II photochemistry, increased stomatal limitation, downregulation of photosynthesis, and the decreased aboveground and belowground biomass under artificial night lighting. Histochemical analysis with 3,3 -diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining indicates the accumulation of in situ superoxide radicals (O 2 − ) and hydrogen peroxide (H 2 O 2 ) in leaves exposed to the lowest level of artificial night lighting compared to control. Moreover, these leaves exposed to artificial night lighting had a lower nighttime respiration rate. These results indicated that HPS lighting during the night may act as a major factor as repressors of the fitness of photosynthesis and growth patterns, via a modification of the photosynthetic light harvesting apparatus.

show abstract

Section: Discussionmentioning

confidence: 99%

Night Light-Adaptation Strategies for Photosynthetic Apparatus in Yellow-Poplar (Liriodendron tulipifera L.) Exposed to Artificial Night Lighting

Kwak

Cheng

et al. 2018

Forests

View full text Add to dashboard Cite

show abstract

“…Although there is no denial that the LHCII antenna undergoes reorganization into the NPQ state, recent data suggest that it does not uncouple energetically from RCII (Johnson and Ruban, 2009;Belgio et al, 2014), as was previously proposed (Holzwarth et al, 2009), which is in total agreement with the earlier established and experimentally confirmed relationship between the yield of PSII and NPQ (Genty et al, 1989). Moreover, it was shown that NPQ protects closed, not open, RCII, which makes this protective strategy economical, as it does not allow much competition between NPQ and RCII traps for energy under low or moderate light intensity .…”

Section: Change: Lhcii Rearrangements/aggregation and The Formation Omentioning

confidence: 99%

Nonphotochemical Chlorophyll Fluorescence Quenching: Mechanism and Effectiveness in Protecting Plants from Photodamage

2016

Self Cite

View full text Add to dashboard Cite

We review the mechanism underlying nonphotochemical chlorophyll fluorescence quenching (NPQ) and its role in protecting plants against photoinhibition. This review includes an introduction to this phenomenon, a brief history of major milestones in our understanding of NPQ, definitions, and a discussion of quantitative measurements of NPQ. We discuss the current knowledge and unknown aspects in the NPQ scenario, including the following: DpH, the proton gradient (trigger); lightharvesting complex II (LHCII), PSII light harvesting antenna (site); and changes in the antenna induced by DpH (change), which lead to the creation of the quencher. We conclude that the minimum requirements for NPQ in vivo are DpH, LHCII complexes, and the PsbS protein. We highlight the most important unknown in the NPQ scenario, the mechanism by which PsbS acts upon the LHCII antenna. Finally, we describe a novel, emerging technology for assessing the photoprotective "power" of NPQ and the important findings obtained through this technology.

show abstract

“…Whole leaves were removed from plants after 45 min dark adaptation and then subjected to three saturating pulses (1000 lmol m -2 s -1 , 0.6 s width). Vacuum infiltration was then performed on leaves with 10 mM Hepes pH 7.5, 150 mM sorbitol and 30 lM DCMU (for more details, see Belgio et al 2012Belgio et al , 2014. 20 s after infiltration, a pre-programmed fast trigrun (Walz, Germany) of 7 lmol m -2 s -1 measuring light intensity was performed.…”

Section: Psii Fast Fluorescence Induction Measurementsmentioning

confidence: 99%

“…These values were taken from a number of previous reports (Pessarakli 2005;Dall'Osto et al 2006;Caffarri et al 2009;Amunts et al 2010). For more details see Table 1 from Belgio et al (2014). Chlorophyll a/b ratios of each photosystem component were taken from Table 2 in Hogewoning et al (2012).…”

Section: Absorption Spectroscopymentioning

confidence: 99%

Photoprotective capacity of non-photochemical quenching in plants acclimated to different light intensities

2015

Self Cite

View full text Add to dashboard Cite

Arabidopsis plants grown at low light were exposed to a gradually increasing actinic light routine. This method allows for the discerning of the photoprotective component of NPQ, pNPQ and photoinhibition. They exhibited lower values of Photosystem II (PSII) yield in comparison to high-light grown plants, and higher calculated dark fluorescence level (F'o calc.) than the measured one (F'o act.). As a result, in low-light grown plants, the values of qP measured in the dark appeared higher than 1. Normally, F'o act. and F'o calc. match well at moderate light intensities but F'o act. becomes higher at increasing intensities due to reaction centre (RCII) damage; this indicates the onset of photoinhibition. To explain the unusual increase of qP in the dark in low-light grown plants, we have undertaken an analysis of PSII antenna size using biochemical and spectroscopic approaches. Sucrose gradient separation of thylakoid membrane complexes and fast fluorescence induction experiments illustrated that the relative PSII cross section does not increase appreciably with the rise in PSII antenna size in the low-light grown plants. This suggests that part of the increased LHCII antenna is less efficiently coupled to the RCII. A model based upon the existence of an uncoupled population LHCII is proposed to explain the discrepancies in calculated and measured values of F'o.

show abstract

Economic photoprotection in photosystem II that retains a complete light-harvesting system with slow energy traps

Cited by 84 publications

References 34 publications

Night Light-Adaptation Strategies for Photosynthetic Apparatus in Yellow-Poplar (Liriodendron tulipifera L.) Exposed to Artificial Night Lighting

Night Light-Adaptation Strategies for Photosynthetic Apparatus in Yellow-Poplar (Liriodendron tulipifera L.) Exposed to Artificial Night Lighting

Nonphotochemical Chlorophyll Fluorescence Quenching: Mechanism and Effectiveness in Protecting Plants from Photodamage

Photoprotective capacity of non-photochemical quenching in plants acclimated to different light intensities

Contact Info

Product

Resources

About