Nitrogen dynamics varies spatially in response to complex interactions among weather, soil, and management practices. Twenty-six spring wheat (Triticum aestivum L.) fields were studied for N losses, nitrous oxide (N 2 O) flux and ammonia (NH 3 ) volatilization, grain yield and protein concentration in farmers fields in Minnesota and North Dakota of the Red River Valley during 2018-2020 growing seasons. Spring wheat grain yield ranged from 2.8 to 9.7 Mg ha -1 with protein concentration ranging between 106 and 180 g kg -1 . Average cumulative N 2 O and NH 3 losses were 415 g N 2 O-N ha -1 and 1.43 kg NH 3 ha -1 , respectively (n = 26). Spring wheat grain yield (r = .53, p = .01) had positive association with May rainfall. Grain protein concentration declined with delay in planting (r = -0.55, p = .01). Linear regression indicates a close association of daily N 2 O flux with soil moisture (r 2 = .23, p < .01), soil inorganic N concentration (r 2 = .02, p = .04), and days after planting (r 2 = .20, p < .01). Soil inorganic N concentration (just after planting) had a positive association with cumulative N 2 O flux (r = .41, p = .03). This study showed rainfall, soil moisture, and inorganic N concentration play a dominant role in N dynamics across the region.