Ecophysiological responses of the biocontrol agent Trichoderma atroviride (T-15603.1) to combined environmental parameters

Schubert, Mark; Mourad, Safer; Fink, Siegfried; Schwarze, Francis W. M. R.

doi:10.1016/j.biocontrol.2008.12.015

Cited by 19 publications

(19 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On basic MEA medium the optimal conditions for growth of Physisporinus vitreus are a w ¼ 0.998, 20 C, and pH 5. This experiments on MEA medium may not represent the hyphal growth rate in situ, but experiments with Trichoderma atroviride (Schubert et al 2009b) using low nutrient agar (LNA) of a similar nutrient composition such as wood (Srinivasan et al 1992) show that the growth rates on LNA medium are about 25 % slower compared to MEA medium e an effect, which can be observed as well in growing colonies of P. vitreus. The use growth rates based on MEA experiments should be acceptable for our purpose.…”

Section: Mycelium (Fungus)mentioning

confidence: 94%

Modelling the hyphal growth of the wood-decay fungus Physisporinus vitreus

Fuhr¹,

Schubert²,

Schwarze³

et al. 2011

Fungal Biology

Self Cite

View full text Add to dashboard Cite

a b s t r a c tThe white-rot fungus, Physisporinus vitreus, degrades the membranes of bordered pits in tracheids and consequently increases the permeability of wood, which is a process that can be used by the wood industry to improve the uptake of wood preservatives and environmentally benign wood modification substances to enhance the use and sustainability of native conifer wood species. To understand and apply this process requires an understanding of how a complex system (fungusewood) interacts under defined conditions. We present a three-dimensional fungal growth model (FGM) of the hyphal growth of P. vitreus in the heartwood of Norway spruce. The model considers hyphae and nutrients as discrete structures and links the microscopic interactions between fungus and wood (e.g. degradation rate and degree of opening of pits) with macroscopic system properties, such penetration depth of the fungus, biomass, and distribution of destroyed pits in early-and latewood.Simulations were compared with experimental data. The growth of P. vitreus is characterized by a stepwise capture of the substrate and the effect of this on wood according to different model parameters is discussed.

show abstract

Section: Mycelium (Fungus)mentioning

confidence: 94%

Modelling the hyphal growth of the wood-decay fungus Physisporinus vitreus

Fuhr¹,

Schubert²,

Schwarze³

et al. 2011

Fungal Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Schubert et al [8,37] statistically determined the growth behavior of P. vitreus, as well as that of T-15603.1, under different principal environmental conditions. The comparison of the lag phase and growth rate of both fungi revealed that even under optimal growth conditions for P. vitreus, T-15603.1 showed a significant shorter lag phase and a higher growth rate.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the interspecific competitive ability of the bioincising fungus Physisporinus vitreus

Schubert

Schwarze

2010

J. Basic Microbiol.

Self Cite

View full text Add to dashboard Cite

A hierarchical set of assays were used to evaluate the interspecific competitive ability of the bioincising fungus Physiporinus vitreus (response species). The competitiveness and growth mode of P. vitreus against a range of blue stain fungi and Trichoderma species (challenge species) was investigated in dual culture tests (a) and a spatially heterogeneous system of tessellation agar (b) on different media. In addition, the robustness of the bioincising process against biotic influences was determined by controlled interaction tests with different inocula of the challenge species (colony-forming units: 10(2), 10(4), 10(6) ml(-1)) on Norway spruce (Picea abies Karst.) heartwood. The results of the dual culture and tessellation agar tests revealed a significant (P ≤ 0.001) antagonistic potential (lethal effect) of the T. atroviride strain (15603.1), whereas no or only a slight effect of the blue stain fungi on P. vitreus was detectable. The interaction tests on Norway spruce heartwood confirmed that an increase in inoculum size of the challenge fungi correlated with an increased negative effect (Spearman's rho coefficient (ρ) = -0.521). Among the challenge species, T-15603.1 revealed the most negative effect on the homogeneous development of P. vitreus on wood and thus on the outcome of the bioincising process. To improve the uniformity of colonization and to reduce the rate of contamination, the incubation of wood in a sealed system (bioreactor) after sterilization is suggested.

show abstract

“…Likewise, explanations for why the bias and accuracy factor method (21) was not used to evaluate performance of the tertiary PMP model can be found in previous publications (15,18,22). An explanation for why only the tertiary PMP model was evaluated and not also the secondary models for lag time and growth rate can be found in a previous publication (17).…”

Section: Sampling Of Chickenmentioning

confidence: 99%

“…Typhimurium definitive type 104 (DT104) on chicken skin as a function of time (0 to 8 h) and temperature (5 to 50°C) (18). This model was validated with the acceptable prediction zone (APZ) method, which has been used to validate other predictive models (1,8,10,15,19,20,22).…”

mentioning

confidence: 99%

Validation of a Predictive Model for Survival and Growth of Salmonella Typhimurium DT104 on Chicken Skin for Extrapolation to a Previous History of Frozen Storage

Oscar

2013

Journal of Food Protection

View full text Add to dashboard Cite

The U.S. Department of Agriculture's tertiary Pathogen Modeling Program (PMP) model for survival and growth of Salmonella enterica ser. Typhimurium definitive type 104 (DT104) on chicken skin stored for 0 to 8 h at 5 to 50°C was evaluated for its ability to predict survival and growth of the same organism on chicken skin after frozen storage for 6 days at -20°C. Experimental design and methods used to collect data for model development (dependent data) were the same as those used to collect data for survival and growth after frozen storage (independent data for extrapolation). This was done to provide a valid comparison of observed and predicted values. The model was classified as providing acceptable predictions of the test data when the proportion of residuals in an acceptable prediction zone (pAPZ) from -1 log (fail-safe) to 0.5 log (fail-dangerous) was ≥0.7. The pAPZ for dependent data, independent data for interpolation, and independent data for extrapolation to a new independent variable of previous frozen storage were all acceptable (pAPZ ≥0.7), with the exception of the pAPZ for dependent data at 50°C, where an unacceptable pAPZ of 0.625 was obtained. Although a majority of observed log counts were less than predicted log counts, indicating that frozen storage of chicken skin for 6 days at -20°C had injured some Salmonella Typhimurium DT104, the injury was not large enough to cause the tertiary PMP model to provide unacceptable predictions. Thus, it was concluded that the tertiary PMP model provided valid predictions of survival and growth of Salmonella Typhimurium DT104 on chicken skin that had a previous history of frozen storage for 6 days at -20°C. Additional research is needed to determine how broadly the model can be applied to other conditions of previous frozen storage.

show abstract

Ecophysiological responses of the biocontrol agent Trichoderma atroviride (T-15603.1) to combined environmental parameters

Cited by 19 publications

References 24 publications

Modelling the hyphal growth of the wood-decay fungus Physisporinus vitreus

Modelling the hyphal growth of the wood-decay fungus Physisporinus vitreus

Evaluation of the interspecific competitive ability of the bioincising fungus Physisporinus vitreus

Validation of a Predictive Model for Survival and Growth of Salmonella Typhimurium DT104 on Chicken Skin for Extrapolation to a Previous History of Frozen Storage

Contact Info

Product

Resources

About