Ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes

Ntougias, Spyridon; Zervakis, Georgios I.; Ehaliotis, Constantinos; Kavroulakis, Nektarios

doi:10.1016/j.resmic.2005.09.010

Cited by 52 publications

(51 citation statements)

References 30 publications

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“…The few available reports concerning microbial diversity of alkaline environments indicate the existence of many physiological groups (Duckworth et al, 1996;Jones et al, 1998;Horikoshi, 1999;Ntougias et al, 2006;Felföldi et al, 2009). The aerobic microbial population of these ecosystems contains organotrophic bacteria, including members of the Bacillus species, which are able to produce a wide range of enzymes used for recycling biopolymers: proteases, lipases, amylases, and cellulases.…”

Section: Discussionmentioning

confidence: 99%

“…Extracts from the alkaline lime (100 g dissolved in 1 l of distilled water, mixed for 20 min, filtered and adjusted to pH 7 and 11 using the appropriate buffer solutions according to Ntougias et al (2006), were solidified with agar and used to estimate the number of cultivable bacteria. All plates were incubated up to 7 days at 25°C.…”

Section: Methodsmentioning

confidence: 99%

“…All experiments were performed at 25°C, unless stated otherwise. Alkaliphilic/ alkalitolerant strains were maintained and grown routinely on alkaline media (Ntougias et al, 2006), composed of the nutritional base described above, 30 g l −1 NaCl, 0.1 mM MgSO 4 and a buffer (Na 2 CO 3 -K 2 HPO 4 ), at pH 10.…”

Section: Methodsmentioning

confidence: 99%

“…Marine environments contain many alkalitolerant bacteria and usually a smaller number of alkaliphiles (Ntougias et al, 2006;Tamura et al, 2013). Alkaliphiles have also been isolated from a number of alkaline wastes, by-products from food processing, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Alkaliphiles have also been isolated from a number of alkaline wastes, by-products from food processing, e.g. potato processing plant effluents (Collins et al, 1983), olive and maize processing wastewater (Ntougias et al, 2006;Sanchez-Gonzalez et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Physiology and Molecular Phylogeny of Bacteria Isolated from Alkaline Distillery Lime

Kalwasińska¹,

Felföldi²,

Walczak³

et al. 2015

Pol J Microbiol

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A b s t r a c tThis paper presents the results of the research on the number, taxonomic composition, and biochemical properties of bacterial strains isolated from the alkaline Solvay distillery lime, deposited at the repository in Janikowo (central Poland). Fifteen strains out of 17 were facultative alkaliphiles and moderate halophiles, and two were alkalitolerants and moderate halophiles. The number of aerobic bacteria cultured in alkaline lime was approximately 10 5 CFU ml -1 , and the total number of bacteria was 10 7 cells g -1 . According to 16S rRNA gene sequence analysis, nine strains belonged to the genus Bacillus, six to the genus Halomonas, one to the genus Planococcus, and one to the genus Microcella. Strains that hydrolyse starch and protein were the most numerous. Esterase (C4) and esterase lipase (C8) were detected in the majority of bacterial strains. Twelve strains exhibited α-glucosidase activity and nine, naphtol-AS-BI-phosphohydrolase activity. The present study proves that alkaliphilic bacteria of this type may constitute a source of potentially useful extremozymes.K e y w o r d s: Bacillus sp., Halomonas sp., alkaline solvay distillery lime, alkaliphiles, halophiles

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Physiology and Molecular Phylogeny of Bacteria Isolated from Alkaline Distillery Lime

Kalwasińska¹,

Felföldi²,

Walczak³

et al. 2015

Pol J Microbiol

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Nesterenkonia

Stackebrandt¹

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Ne.ste.ren.ko'ni.a. N.L. fem. n. Nesterenkonia honoring Olga Nesterenko, a Ukrainian microbiologist. Actinobacteria / Actinobacteria / Micrococcales / Micrococcaceae / Nesterenkonia Cells may consist of short rods sometimes showing branching, or of cocci. Gram‐stain‐positive. Non‐encapsulated. Endospores are not formed. Chemo‐organotrophic ; metabolism is strictly respiratory . Aerobic. Catalase‐positive. Moderately halophilic or halotolerant. Some species are alkaliphilic or alkalitolerant. Mesophilic. Peptidoglycan type is A4α ( l ‐ Lys–Gly – l ‐ Glu , l ‐ Lys – l ‐ Glu or l ‐ Lys–Gly – d ‐ Asp ). Mycolic acids are absent. Menaquinones with seven , eight , and nine completely unsaturated isoprene units ( MK‐7 , MK‐8 , and MK‐9 ) predominate . The phospholipids are diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol. The cellular fatty acids are iso and anteiso‐branched fatty acids, with C 15:0 anteiso and C 17:0 anteiso predominating. Phylogenetically a member of the family Micrococcaceae . DNA G + C content ( mol %): 64–72. Type species : Nesterenkonia halobia Stackebrandt, Koch, Gvozdiak and Schumann 1995, 689 VP ; basonym Micrococcus halobius Onishi and Kamekura 1972, 235 AL .

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H alotalea

Ntougias¹,

Zervakis²

2019

Bergey's Manual of Systematics of Archaea and Bacteria

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Ha.lo.ta'le.a. Gr. n. hals , halos salt; L. fem. n. talea a staff, rod; N.L. fem. n. Halotalea , a rod‐shaped organism living in saline conditions. Proteobacteria / Gammaproteobacteria / Oceanospirillales / Halomonadaceae / Halotalea The genus Halotalea , classified within the family Halomonadaceae , order Oceanospirillales , and in the class Gammaproteobacteria , currently consists of a single species, Halotalea alkalilenta , isolated from alkaline alperujo, an alkaline sludge‐like olive mill waste sampled in the vicinity of Toplou Monastery, Sitia (Crete), Greece. Cells are nonsporulating Gram‐negative, motile by peritrichous flagella, rods. Colonies on agar plates are small and nonpigmented, coloring pale yellow. The organism is alkalitolerant, growing up to pH 11; extremely halotolerant, tolerating up to 2.6 M NaCl; and sugar tolerant, resisting up to 2.5 and 1.7 M d ‐glucose and maltose, respectively. It is a mesophilic facultative anaerobe, which lacks cytochrome c oxidase activity and displays delayed fermentation ability on glucose and dulcitol. It is chemoorganotrophic, capable of degrading phenolics, which utilizes a range of amino acids, sugars, alcohols, and organic acids as sole carbon and energy sources. Ubiquinone‐9 is the only quinone detected, and C 18:1 ω7 c , C 16:0 , C 19:0 cyclo ω8 c , C 12:0 3‐OH, and C 16:1 ω7 c /iso‐C 15:0 2‐OH are the major membrane fatty acids determined. Representatives have been isolated from diverse ambient and engineered environments, including wastes, marine specimens, rhizosphere, and plant material. DNA G + C content (mol%) : 64.3–64.6 (genome sequences). Type species : Halotalea alkalilenta Ntougias, Zervakis and Fasseas 2007, 1981 VP .

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Ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes

Cited by 52 publications

References 30 publications

Physiology and Molecular Phylogeny of Bacteria Isolated from Alkaline Distillery Lime

Physiology and Molecular Phylogeny of Bacteria Isolated from Alkaline Distillery Lime

Nesterenkonia

H alotalea

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