Ecophysiology of “
            <i>Halarsenatibacter silvermanii</i>
            ” Strain SLAS-1
            <sup>T</sup>
            , gen. nov., sp. nov., a Facultative Chemoautotrophic Arsenate Respirer from Salt-Saturated Searles Lake, California

Js, Blum; Han, Sunyoung; Lanoil, Brian; Witte, Barbara; Fr, Tabita; Langley, Sasha A.; Tj, Beveridge; Jahnke, L. L.; Rs, Oremland

doi:10.1128/aem.01433-09

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this case, sequences similar to a cultured representative, Desulfohalobium utahense , known to contain iso‐17:1 ∆7 (Ollivier et al ., ; Jakobsen et al ., ) were detected in the P and OB layers (P9_LJL_017; Table S2), however, a relatively small amount of iso‐17:1 was present in these layers. Iso‐17:1 has also been detected in some Firmicutes (Eder et al ., ; Kim et al ., ; Blum et al ., ) and Flavobacteriales (Donachie et al ., ; Lau et al ., ), and sequences clustering with these groups were found throughout the crust. However, the highest levels of iso‐17:1 were in the upper oxic crust (T + O and G) and best correlate with Stenotrophomonas clones (P9_LJL_0205_007) which make up a significant portion of the T, O, and G + P clone libraries.…”

Section: Discussionmentioning

confidence: 99%

Molecular and lipid biomarker analysis of a gypsum‐hosted endoevaporitic microbial community

et al. 2013

View full text Add to dashboard Cite

Modern evaporitic microbial ecosystems are important analogs for understanding the record of earliest life on Earth. Although mineral-depositing shallow-marine environments were prevalent during the Precambrian, few such environments are now available today for study. We investigated the molecular and lipid biomarker composition of an endoevaporitic gypsarenite microbial mat community in Guerrero Negro, Mexico. The 16S ribosomal RNA gene-based phylogenetic analyses of this mat corroborate prior observations indicating that characteristic layered microbial communities colonize gypsum deposits world-wide despite considerable textural and morphological variability. Membrane fatty acid analysis of the surface tan/orange and lower green mat crust layers indicated cell densities of 1.6 × 10(9) and 4.2 × 10(9) cells cm(-3) , respectively. Several biomarker fatty acids, ∆7,10-hexadecadienoic, iso-heptadecenoic, 10-methylhexadecanoic, and a ∆12-methyloctadecenoic, correlated well with distributions of Euhalothece, Stenotrophomonas, Desulfohalobium, and Rhodobacterales, respectively, revealed by the phylogenetic analyses. Chlorophyll (Chl) a and cyanobacterial phylotypes were present at all depths in the mat. Bacteriochlorophyl (Bchl) a and Bchl c were first detected in the oxic-anoxic transition zone and increased with depth. A series of monomethylalkanes (MMA), 8-methylhexadecane, 8-methylheptadecane, and 9-methyloctadecane were present in the surface crust but increased in abundance in the lower anoxic layers. The MMA structures are similar to those identified previously in cultures of the marine Chloroflexus-like organism 'Candidatus Chlorothrix halophila' gen. nov., sp. nov., and may represent the Bchl c community. Novel 3-methylhopanoids were identified in cultures of marine purple non-sulfur bacteria and serve as a probable biomarker for this group in the lower anoxic purple and olive-black layers. Together microbial culture and environmental analyses support novel sources for lipid biomarkers in gypsum crust mats.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular and lipid biomarker analysis of a gypsum‐hosted endoevaporitic microbial community

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Bacteria such as Geoalkalibacter ferrihydriticus can only grow and reduce Fe(III) in up to 50 g/liter Ϫ1 NaCl (103). The same applies to several Bacillus strains (31,88) as well as to an Fe(III)-reducing isolate from the sediment of the hypersaline Lake Chaka (China), because the strain originated from 880-cm depth, where salinity was only slightly elevated compared to freshwater (29).…”

mentioning

confidence: 99%

“…To date, a few isolates from hypersaline environments have been reported to be capable of Fe(III) reduction. Often the isolates were shown to reduce dissolved Fe(III) but were not tested for the reduction of any solid Fe(III) phases (63,87,88), which represent the dominant form of Fe(III) at neutral or even alkaline pH values of most salt lake environments (85). In other cases, isolates were found to be halotolerant rather than halophilic.…”

mentioning

confidence: 99%

Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia

Emmerich

Bhansali

Lösekann-Behrens

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

ABSTRACTThe extreme osmotic conditions prevailing in hypersaline environments result in decreasing metabolic diversity with increasing salinity. Various microbial metabolisms have been shown to occur even at high salinity, including photosynthesis as well as sulfate and nitrate reduction. However, information about anaerobic microbial iron metabolism in hypersaline environments is scarce. We studied the phylogenetic diversity, distribution, and metabolic activity of iron(II)-oxidizing and iron(III)-reducingBacteriaandArchaeain pH-neutral, iron-rich salt lake sediments (Lake Kasin, southern Russia; salinity, 348.6 g liter−1) using a combination of culture-dependent and -independent techniques. 16S rRNA gene clone libraries forBacteriaandArchaearevealed a microbial community composition typical for hypersaline sediments. Most-probable-number counts confirmed the presence of 4.26 × 102to 8.32 × 103iron(II)-oxidizingBacteriaand 4.16 × 102to 2.13 × 103iron(III)-reducing microorganisms per gram dry sediment. Microbial iron(III) reduction was detected in the presence of 5 M NaCl, extending the natural habitat boundaries for this important microbial process. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of totalBacteria, totalArchaea, and species dominating the iron(III)-reducing enrichment cultures (relatives ofHalobaculum gomorrense,Desulfosporosinus lacus, and members of theBacilli) were highest in an iron oxide-rich sediment layer. Combined with the presented geochemical and mineralogical data, our findings suggest the presence of an active microbial iron cycle at salt concentrations close to the solubility limit of NaCl.

show abstract

“…M (Amoozegar et al 2013) Halobacteroidaceae (Oren 2008). El estudio de este grupo ha sido hasta cierto punto olvidado en comparación con los otros grupos monofiléticos que contienen en su totalidad microorganismos halófilos, lo cual se refleja en el poco número de especies descritas hasta el momento, 14 géneros y 30 especies (Blum et al 2009;Hedi et al 2009;Oren 2008;Zhilina et al 2012). El orden Halanaerobiales está formado por anaerobios obligados con metabolismo fermentativo u homoacetogénico, y la mayoría ha sido aislada de tanques de fermentación de azúcares o aminoácidos.…”

Section: En El Dominiounclassified

Análisis comparativo de la expresión de proteinas de Tislia consotesis en respuesta a cambios de la salinidad externa

Labrador¹

View full text Add to dashboard Cite

En este estudio se evaluó el perfil de expresión de proteínas de Tistlia consotensis bajo condiciones variables de salinidad (0 y 40 g·l-1 NaCl) empleando dos estrategias proteómicas. En la primera estrategia, se utilizó la técnica de electroforesis bidimensional para realizar un análisis cualitativo de la expresión de proteínas de T. consotensis bajo las salinidades evaluadas y posteriormente se realizó un estudio detallado utilizando una estrategia proteogenómica, que consistió en una anotación rápida de los genes cuyos productos se detectaron mediante espectrometría de masas. El análisis cualitativo de la expresión de proteínas de T. consotensis en respuesta a cambios en la salinidad del medio permitió determinar que esta bacteria halotolerante responde al estrés osmótico con cambios en los niveles de expresión de sus proteínas. Asimismo, el análisis del exoproteoma y del proteoma celular de T. consotensis, utilizando el enfoque proteogenómico, permitió la detección de proteínas específicas a 0 y 40 g·l-1 NaCl involucradas en los mecanismos de adaptación de T. consotensis bajo estrés hipo e hiper-osmótico a través de la comparación con el perfil de expresión en la condición de crecimiento a la salinidad óptima, 5 g·l-1 NaCl. En ausencia de sal, T. consotensis aumenta la producción de proteínas de estrés general y proteínas implicadas en la modulación de la composición de membrana celular. En respuesta a un incremento de la salinidad, T. consotensis aumenta la abundancia de proteínas involucradas con procesos de señalización, responsables de detectar eficientemente cambios en la concentración de Cl-, y proteínas relacionadas con diferentes tipo de sistemas de transporte encargados de acumular solutos compatibles y mantener el exceso de Na+ fuera de la célula. Este estudio proporcionó una visión detallada de la adaptación celular, en términos de perfiles proteómicos, a cambios en la salinidad externa en T. consotensis, sugiriendo que esta bacteria halotolerante combina diferentes estrategias novedosas para contrarrestar los cambios de salinidad en su hábitat.

show abstract

Ecophysiology of “ Halarsenatibacter silvermanii ” Strain SLAS-1 ^T , gen. nov., sp. nov., a Facultative Chemoautotrophic Arsenate Respirer from Salt-Saturated Searles Lake, California

Cited by 4 publications

References 0 publications

Molecular and lipid biomarker analysis of a gypsum‐hosted endoevaporitic microbial community

Molecular and lipid biomarker analysis of a gypsum‐hosted endoevaporitic microbial community

Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia

Análisis comparativo de la expresión de proteinas de Tislia consotesis en respuesta a cambios de la salinidad externa

Contact Info

Product

Resources

About

Ecophysiology of “ Halarsenatibacter silvermanii ” Strain SLAS-1 T , gen. nov., sp. nov., a Facultative Chemoautotrophic Arsenate Respirer from Salt-Saturated Searles Lake, California

Cited by 4 publications

References 0 publications

Molecular and lipid biomarker analysis of a gypsum‐hosted endoevaporitic microbial community

Molecular and lipid biomarker analysis of a gypsum‐hosted endoevaporitic microbial community

Abundance, Distribution, and Activity of Fe(II)-Oxidizing and Fe(III)-Reducing Microorganisms in Hypersaline Sediments of Lake Kasin, Southern Russia

Análisis comparativo de la expresión de proteinas de Tislia consotesis en respuesta a cambios de la salinidad externa

Contact Info

Product

Resources

About

Ecophysiology of “ Halarsenatibacter silvermanii ” Strain SLAS-1 ^T , gen. nov., sp. nov., a Facultative Chemoautotrophic Arsenate Respirer from Salt-Saturated Searles Lake, California