Research handbook on start-up incubation ecosystems make up an ecological system or ecosystem. Similar to natural ecosystems, entrepreneurial ecosystems comprise complex and diverse networks of organizations, individuals, and other environmental components that interact to influence entrepreneurial activity and economic functioning in a particular context, region, or locality. A fundamental feature of ecosystems, either natural or entrepreneurial, is that each organism/organization must use some energy/resources that they receive from other organisms/ organizations to survive. Like organisms in natural ecosystems, innovative new firms, often referred to as start-ups, are embedded in an ecosystem which helps or hinders their access to resources and thus influences their development and impact (Spigel & Harrison, 2018; Spilling, 1996). Many initiatives have been implemented to support start-ups, such as incubators (Bergek & Norrman, 2008), accelerators (Shankar & Shepherd, 2018), outside assistants (Chrisman & McMullan, 2004) and government funding mechanisms (Rasmussen & Sørheim, 2012). These initiatives aim to provide "life energy" in the form of various resources for start-ups to survive, grow and contribute to social and economic impact. Studies often look at these support mechanisms in isolation, while their effectiveness depends on the context in which they operate (Brown, Gregson, & Mason, 2016). Different natural ecosystems, for example, a tropical evergreen forest and a desert, differ in both the numbers and types of species as well as in physical components such as vegetation, soil type, rainfall, and temperature. Such contextual differences make it hard to compare populations in different locations because the variation in species richness and abundance is influenced by indigenous environmental factors (Naeem et al., 1999). Similarly, when comparing firms in two different ecosystems, the variations in the number and success of start-ups is partly due to differences in various framework conditions, available resources, and support mechanisms. While firms in poorer ecosystems with weaker prospects for growth seem to be less successful at first sight, they may be quite effective in adjusting their goals and means to their environment. By the same token, policymakers must consider the characteristics of a particular context before setting economic goals or emulating practices of ecosystems with entirely different resource profiles. Hence, the ecosystem analogy is valuable to entrepreneurship research because it conceptually addresses the need for more knowledge on how start-ups interact with and are in turn influenced by, their environment (Audretsch, Cunningham,