When growing wide-row crops on sloped lands, there is significant surface runoff. In relation to the runoff process, potatoes are classified as a risk crop. This study aimed to grow potatoes in the Bohemian-Moravian Highlands, where the protection zone of the water supply reservoir of Švihov is also located. At selected experimental areas, water samples were taken after precipitation events when surface runoff and water erosion occurred. These samples were analysed (nitrates, total P, and selected pesticides used for potato growing) in an accredited laboratory. We located three different variants of nitrogen fertilisation in each experimental area. Precipitation and the amount of water from surface runoff after each higher precipitation event were also measured in the experimental areas. By knowing the acreage of each experimental area, the volume of surface runoff water and the concentration of nitrates, phosphorus, and pesticides, it was possible to calculate the balance of these substances. We also calculated the percentage of surface runoff. The results imply that a new potato cultivator in the technology of stone windrowing should be designed for weed control as part of a weed control system with reduced herbicide application requirements. Innovative agrotechnical processes reducing pollution of water sources by phosphorus and nitrates should also be enhanced. These are based on a precise application of mineral fertiliser into the root area of plants within the period of an intensive intake of nutrients.