EcoTILLING for the identification of allelic variants of melon eIF4E, a factor that controls virus susceptibility

Nieto, Cristina; Piron, Florence; Dalmais, Marion; Marco, Cristina F.; Moriones, Enrique; Goméz-Guillamón, M. L.; Truniger, Verónica; Gómez, Pedro Sánchez; García-Más, Jordi; Aranda, Miguel A.; Bendahmane, Abdelhafid

doi:10.1186/1471-2229-7-34

Cited by 132 publications

(105 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…An EcoTILLING analysis of 80 banana accessions identified 870 novel alleles in 14 genes (Till et al, 2010). Variants in eIF4E that conferred virus resistance were discovered by the genetic screening of 113 melon accessions and 233 pepper accessions (Nieto et al, 2007;Ibiza et al, 2010). High-throughput sequencing of eIF4E from 92 tomato lines identified six allelic variants, although virus resistance was not reported (Rigola et al, 2009).…”

Section: Genetic Screening and Cultivar Developmentmentioning

confidence: 99%

“…Natural allelic variation found in eIF4E genes provided melon with resistance to melon necrotic spot virus (Nieto et al, 2007) and peppers with resistance to potato virus Y and tobacco etch virus (Ibiza et al, 2010;Jeong et al, 2011). An EMSinduced splice site mutation in tomato eIF4E conferred resistance to strains of potato virus Y and pepper mottle virus .…”

Section: Horticultural Traits Developed Through Targeted Mutation Brementioning

confidence: 99%

“…Because the natural allelic diversity of melon eIF4E was found to be limited, mutagenesis was proposed as a means to obtain novel eIF4E alleles (Nieto et al, 2007). Genetic diversity in melon and other plants can be induced using chemical agents such as ethyl methanesulfonate (EMS) or physical agents such as gamma rays .…”

Section: Genetic Screening and Cultivar Developmentmentioning

confidence: 99%

“…For clarity in this review, TILLING will refer to detection through mismatchspecific nuclease cleavage, which was used for all TILLING screens shown in Table 1. EcoTILLING is a technically similar approach that has been used to identify natural polymorphisms in target genes of melon, bean, sunflower, and peppers (Nieto et al, 2007;Galeano et al, 2009;Fusari et al, 2010;Ibiza et al, 2010).…”

Section: Detection Of Natural or Induced Polymorphism In Candidate Genesmentioning

confidence: 99%

See 3 more Smart Citations

Targeted mutation breeding of horticultural plants

Wilde¹,

Chen²,

Jiang³

et al. 2012

Emir. J. Food Agric

View full text Add to dashboard Cite

Mutation breeding can be enhanced by genetic selection for novel alleles. Through targeted mutation breeding, genotypes with induced or natural mutations in candidate genes are identified for cultivar development. For most horticultural plants, targeted mutation breeding may be a more economically feasible approach to trait development than through transgenic technology. Substantial progress has been made in applying targeted mutation breeding to horticulture and this review summarizes recently published work in this area. To date, at least 16 horticultural crops have been screened for natural or induced allelic diversity in over 100 candidate genes. This approach has resulted in traits of commercial use, such as longer shelf-life (tomato, melon), improved starch quality (potato), and virus-resistance (peppers, tomato). Advances in genome sequencing and genetic screening will facilitate the development of cultivars with value-added traits derived from candidate gene polymorphisms.

show abstract

Section: Genetic Screening and Cultivar Developmentmentioning

confidence: 99%

Section: Horticultural Traits Developed Through Targeted Mutation Brementioning

confidence: 99%

Section: Genetic Screening and Cultivar Developmentmentioning

confidence: 99%

Section: Detection Of Natural or Induced Polymorphism In Candidate Genesmentioning

confidence: 99%

See 2 more Smart Citations

Targeted mutation breeding of horticultural plants

Wilde¹,

Chen²,

Jiang³

et al. 2012

Emir. J. Food Agric

View full text Add to dashboard Cite

show abstract

“…One such example is the eIF4E gene that has been found to be associated with virus resistance in many plant species (Nicaise et al 2003;Gao et al 2004;Yoshii et al 2004;Kang et al 2005;Kanyuka et al 2005;Stein et al 2005;Nieto et al 2006Nieto et al , 2007Ibiza et al 2010;Naderpour et al 2010;Piron et al 2010), including resistance to potato virus Y in potato, tomato, and pepper (Ruffel et al 2002(Ruffel et al , 2005Cavatorta et al 2011;Duan et al 2012). Variants of eIF4E confer resistance to PVY in the potato wild species relatives S. chacoense, S. demissum, and S. etuberosum , permitting the eventual use of this gene in future biotech potato varieties.…”

Section: Resistance To Biotic and Abiotic Stressesmentioning

confidence: 99%

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

et al. 2015

View full text Add to dashboard Cite

Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato is an intensively managed crop, requiring irrigation, fertilization, and frequent pesticide applications in order to obtain the highest yields possible. Important traits are easy to find in wild relatives of potato, but their introduction using traditional breeding can take 15-20 years. This is due to sexual incompatibility between some wild and cultivated species, a desire to remove undesirable wild species traits from adapted germplasm, and difficulty in identifying broadly applicable molecular markers. Fortunately, potato is amenable to propagation via tissue culture and it is relatively easy to introduce new traits using currently available biotech transformation techniques. For these reasons, potato is arguably the crop that can benefit most by modern biotechnology. The benefits of biotech potato, such as limited gene flow to conventionally grown crops and weedy relatives, the opportunity for significant productivity and nutritional quality gains, and reductions in production cost and environmental impact, have the potential to influence the marketability of newly developed varieties. In this review we will discuss current and past efforts to develop biotech potato varieties, traits that could be impacted, and the potential effects that biotech potato could have on the industry.Resumen La papa es el cultivo hortícola más importante en el mundo, con cerca de 400 millones de toneladas producidas a nivel mundial anualmente, acreditando la estabilidad en el suministro de alimentos e impacto socioeconómico. En general, la papa es un cultivo manejado intensivamente, que requiere riego, fertilización y aplicaciones frecuentes de plaguicidas para obtener los más altos rendimientos posibles. Los caracteres importantes son fáciles de encontrar en parientes silvestres de la papa, pero su introducción usando el mejoramiento tradicional puede llevar de 15 a 20 años. Esto es debido a la incompatibilidad sexual entre algunas especies silvestres y cultivadas, el deseo para eliminar características indeseables de las especies silvestres del germoplasma adaptado, y la dificultad en la identificación de marcadores moleculares aplicables ampliamente. Afortunadamente, la papa es receptiva a la propagación por cultivo de tejidos y es relativamente fácil la introducción de nuevos caracteres usando técnicas biotecnológicas de transformación actualmente disponibles. Por estas razones, la papa es probablemente el cultivo que se puede beneficiar mayormente por la biotecnología moderna. Los beneficios de la papa biotecnológica, como el flujo genético limitado a cultivos que se siembran convencionalmente y a los parientes como malezas, la oportunidad para productividad significativa y logros en calidad nutricional, y las reducciones en los costos de producción e impacto ambiental, tienen el potencial para influenciar la comercialización...

show abstract