Ecotopic over‐expression of PoCHS from Paeonia ostii altered the fatty acids composition and content in Arabidopsis thaliana

Abstract

Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthetic pathway and has been studied in many plants, but the function of the CHS gene has not been well characterized in Paeonia ostii. In this study, we obtained a CHS homolog gene from P. ostii, which possessed the putative conserved amino acids of chalcone synthase by multiple alignment analysis and demonstrated the highest expression in developing seeds. In vitro assays of the recombinant PoCHS protein confirmed enzymatic activity using malonyl-CoA and 4-coumaroyl-CoA as substrates, and the optimal pH and reaction temperature were 7.5 and 40 C, respectively. Furthermore, ectopic over-expression of PoCHS in Arabidopsis up-regulated the expression levels of genes involved in seed development (ABI), glycolysis (PKp2, PDH-E1a, and SUS2/3), and especially fatty acid biosynthesis (BCCP2, CAC2, CDS2, FatA, and FAD3).This resulted in an increased unsaturated fatty acid content, especially α-linolenic acid, in transgenic Arabidopsis seeds. In this study, we examined the functions of CHS homolog of P. ostii and demonstrated its new function in seed fatty acid biosynthesis.

Integrated metabolomics and transcriptomic analysis of the flavonoid regulatory networks in Sorghum bicolor seeds

“…Eight chalcone synthase genes (CHS) involved in flavonoid biosynthesis were identified and up-regulated in HC4. Chalcone synthase, a key enzyme in the flavonoid biosynthetic pathway, has been studied in many plants [31]. The structural gene expression levels of chalconesynthase (CHS) and chalcone isomerase (CHI) were highly similar and significantly positively correlated with anthocyanin accumulation rate in wild Lycium ruthenicum Murr [32].…”