Ecotoxico-lipidomics: An emerging concept to understand chemical-metabolic relationships in comparative fish models

Dreier, David A.; Bowden, John A.; Aristizabal‐Henao, Juan J.; Denslow, Nancy D.; Martyniuk, Christopher J.

doi:10.1016/j.cbd.2020.100742

Cited by 21 publications

(26 citation statements)

References 86 publications

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“…Additional studies that identify other potential linkages between lipidomic changes and molecular end points, and that show correlation with whole organism level effects, will further aid the development of lipidomics for environmental monitoring. 6 Opportunities to make such connections will increase as the field of untargeted lipidomics matures, and with it, we will have an improved ability to routinely collect lipid structural information. Indeed, while the current study serves as an extensive characterization of the ZFL lipidome and presents the potential of this model ecological and biomedical cell line for understanding exposures to stressors, the complexity of the lipidome offers significant additional opportunities.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…In contrast, lipids (which are primarily hydrophobic) have only recently begun to receive considerable attention despite displaying enormous biological complexity and being central to numerous cellular functions. 6 Constituting roughly 8−20% of the dry weight in animal cells, 10 lipids serve as "building blocks" of cellular and subcellular membranes and are vital for energy storage, cell signaling, endocrine system regulation, cell growth, and numerous other important roles. 11 These functions are regulated by an interconnected network of processes including fatty acid uptake, de novo lipid synthesis and modification, lipid catabolism (e.g., fatty acid β-oxidation), and cell secretion, 12,13 any of which may be impacted by exposure to environmental contaminants.…”

Section: ■ Introductionmentioning

confidence: 99%

“…), amino acid metabolism, nucleotide metabolism, the urea cycle, and the pentose phosphate pathway. In contrast, lipids (which are primarily hydrophobic) have only recently begun to receive considerable attention despite displaying enormous biological complexity and being central to numerous cellular functions. − …”

Section: Introductionmentioning

confidence: 99%

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Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams

Zhen

Teng

Mosley

et al. 2021

Environ. Sci. Technol.

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Surface waters often contain a variety of chemical contaminants potentially capable of producing adverse outcomes in both humans and wildlife due to impacts from industrial, urban, and agricultural activity. Here, we report the results of a zebrafish liver (ZFL) cell-based lipidomics approach to assess the potential ecotoxicological effects of complex contaminant mixtures using water collected from eight impacted streams across the United States mainland and Puerto Rico. We initially characterized the ZFL lipidome using high resolution mass spectrometry, resulting in the annotation of 508 lipid species covering 27 classes. We then identified lipid changes induced by all streamwater samples (nonspecific stress indicators) as well as those unique to water samples taken from specific streams. Subcellular impacts were classified based on organelle-specific lipid changes, including increased lipid saturation (endoplasmic reticulum stress), elevated bis(monoacylglycero)phosphate (lysosomal overload), decreased ubiquinone (mitochondrial dysfunction), and elevated ether lipids (peroxisomal stress). Finally, we demonstrate how these results can uniquely inform environmental monitoring and risk assessments of surface waters.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams

Zhen

Teng

Mosley

et al. 2021

Environ. Sci. Technol.

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“…However, it provided specific and precise measurements of a large number of components that can endorse highthroughput and in-depth phenotyping. For comparison, 1 H NMR metabolomics allows the global quantification of the CH2-CH2 or CH2-CH=CH fatty acid bounds (Marchand et al, 2018), when LC-MS metabolomics can discriminate and characterize hundreds of different lipids belonging to various sub-classes (Dreier et al, 2020), providing undoubtedly more informative and discriminant features. In addition, multi-omics integration tools, such as MixOmic DIABLO (Singh et al, 2019), provides innovative solutions for the comparison of heterogeneous dataset matrices obtained from a single set of samples or individuals (supplementary figures S6-S7).…”

Section: Comparison Of 1 H Nmr and Lc-ms Metabolomics For Fish Liversmentioning

confidence: 99%

Fish metabolome from sub-urban lakes of the Paris area (France) and potential influence of noxious metabolites produced by cyanobacteria

Marie¹,

Leloup²,

Bonin³

et al. 2020

Preprint

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The recent democratisation of high-throughput molecular phenotyping allows the rapid expansion of promising untargeted multi-dimensional approaches (e.g. epigenomics, transcriptomics, proteomics, metabolomics, as well as microbiome metabarcoding), that now represent innovative perspectives for environmental assessments. Indeed, when developed for ecologically relevant species, these emerging omics analyses may present valuable alternatives for the development of novel generations of ecological indicators, that in turn could provide early warnings of eco(toxico)logical impairments. This pilot study investigates the bio-indicative potential of different multi-metric tools based on different high-throughput molecular phenotyping approaches (i.e. metabarcoding of the intestine microbiome, and liver metabolomics by nuclear magnetic resonance (NMR) and liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) on two sentinel fish species (Perca fluviatilis and Lepomis gibbosus) from a set of eight water bodies of the peri-urban area of Paris (France). We show that the LC-MS metabolome dataset allows remarkably clear separation of individuals according to the species but also according to their respective sampling lakes. Interestingly, the similar variations of Perca and Lepomis metabolomes occur locally indicating that local environmental constraints drive the observed metabolome variations beyond their obvious genetic differences. Thus, the development of such reliable molecular phenotyping for environmental monitoring constitutes a promising and innovative bio-indicative tool for environmental assessment.

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Exploring the Relationship Among Lipid Profile Changes, Growth, and Reproduction in Folsomia candida Exposed to Teflubenzuron Over Time

Ilyaskina,

Fernandes,

Berg

et al. 2024

Enviro Toxic and Chemistry

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The integration of untargeted lipidomics approaches in ecotoxicology has emerged as a strategy to enhance the comprehensiveness of environmental risk assessment. Although current toxicity tests with soil microarthropods focus on species performance, that is, growth, reproduction, and survival, understanding the mechanisms of toxicity across all levels of biological organization, from molecule to community is essential for informed decision‐making. Our study focused on the impacts of sublethal concentrations of the insecticide teflubenzuron on the springtail Folsomia candida. Untargeted lipidomics was applied to link changes in growth, reproduction, and the overall stress response with lipid profile changes over various exposure durations. The accumulation of teflubenzuron in organisms exposed to the highest test concentration (0.035 mg a.s. kg–1 soil dry wt) significantly impacted reproductive output without compromising growth. The results suggested a resource allocation shift from reproduction to size maintenance. This hypothesis was supported by lipid shifts on day 7, at which point reductions in triacylglycerol and diacylglycerol content corresponded with decreased offspring production on day 21. The hypermetabolism of fatty acids and N‐acylethanolamines on days 2 and 7 of exposure indicated oxidative stress and inflammation in the animals in response to teflubenzuron bioaccumulation, as measured using high‐performance liquid chromatography–tandem mass spectrometry. Overall, the changes in lipid profiles in comparison with phenotypic adverse outcomes highlight the potential of lipid analysis as an early‐warning tool for reproductive disturbances caused by pesticides in F. candida. Environ Toxicol Chem 2024;00:1–12. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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Ecotoxico-lipidomics: An emerging concept to understand chemical-metabolic relationships in comparative fish models

Cited by 21 publications

References 86 publications

Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams

Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams

Fish metabolome from sub-urban lakes of the Paris area (France) and potential influence of noxious metabolites produced by cyanobacteria

Exploring the Relationship Among Lipid Profile Changes, Growth, and Reproduction in Folsomia candida Exposed to Teflubenzuron Over Time

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