Ecotoxicological Assessment of Aquatic Sediments With Caenorhabditis Elegans (Nematoda)—a Method for Testing Liquid Medium and Whole-Sediment Samples

Traunspurger, Walter; Haitzer, Markus; Höss, Sebastian; Beier, Stefanie; Ahlf, Wolfgang; Steinberg, Christian

doi:10.1897/1551-5028(1997)016<0245:eaoasw>2.3.co;2

Cited by 67 publications

(96 citation statements)

References 32 publications

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“…In the United States and Canada, macroinvertebrates have mostly been used for whole sediment toxicity testing [10][11][12]; standardized sediment contact tests (SCTs) with organisms from other trophic levels did not exist until recently. However, within the last two decades, new SCTs have been developed in Europe using a broader range of organisms, such as bacteria [13][14][15], yeast [16], nematodes [17,18], fish embryos [19,20], and macrophytes [21], with some of them being standardized (ISO 10872; ISO/DIS 16191; ISO/DIS 10871) and approved for assessing the toxicity of contaminated freshwater sediments [22,23].…”

Section: Introductionmentioning

confidence: 99%

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Feiler

Höss

Ahlf

et al. 2012

Environmental Toxicology and Chemistry

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Abstract-A sediment contact test (SCT) battery consisting of five ecotoxicological test systems was applied to 21 native freshwater sediments characterized by a broad variety of geochemical properties and anthropogenic contamination. Higher plants (Myriophyllum aquaticum), nematodes (Caenorhabditis elegans), oligochaetes (Lumbriculus variegatus), zebrafish embryos (Danio rerio), and bacteria (Arthrobacter globiformis), representing various trophic levels and exposure pathways, were used as test organisms. The test battery detected sediment toxicity caused by anthropogenic pollution, whereas the various tests provided site-specific, nonredundant information to the overall toxicity assessment. Based on the toxicity pattern derived from the test battery, the sediments were classified according to a newly proposed classification system for sediment toxicity assessment. The SCT-derived classification generally agreed well with the application of consensus-based sediment quality guidelines (SQGs), especially with regard to sediments with high toxic potential. For sediments with low to medium toxic potential, the SQGs often underestimated the toxicity that was detected by the SCTs, underpinning the need for toxicity tests in sediment quality assessment. Environ. Toxicol. Chem. 2013;32:144-155. # 2012 SETAC

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Section: Introductionmentioning

confidence: 99%

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Feiler

Höss

Ahlf

et al. 2012

Environmental Toxicology and Chemistry

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“…The nematode Caenorahbditis elegans has been utilised widely for toxicity testing of field samples (Mutwakil et al 1997;Traunspurger et al 1997;Power and de Pomerai , 2001), as well as single and mixed toxicants (Leung et al 2008), including pesticides ranging from the fungicide captan (Candido and Jones, 1996) to a series of organophosphates (Rajini et al 2008). Several different types of assay are available, including whole-organism endpoints such as growth, reproduction and motility (Dhawan et al 1999; Thompson fully characterised somatic cell lineage (Sulston et al 1983), complete genome sequence (C. elegans Sequencing Consortium 1998), and the possibility of genome-wide RNA interference by feeding (Kamath et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Use of transgenic GFP reporter strains of the nematode Caenorhabditis elegans to investigate the patterns of stress responses induced by pesticides and by organic extracts from agricultural soils

et al. 2012

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A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription.For more information, please contact eprints@nottingham.ac.uk reporters to investigate the toxicity of soil pore water from two agricultural sites in south-east Spain, designated P74 (used for cauliflower production, but significantly metal contaminated) and P73(used for growing lettuce, but with only background levels of metals). Both soil pore water samples induced all five test genes to varying extents, yet artificial mixtures containing all major metals present had essentially no effect on these same transgenes. Soluble organic contaminants present in the pore water were extracted with acetone and dichloromethane, then after evaporation of the solvents, the organic residues were redissolved in ultrapure water to reconstitute the soluble organic components of the original soil pore water. These organic extracts induced transgene expression at similar or higher levels than the original pore water. Addition of the corresponding metal mixtures had either no effect, or reduced transgene expression towards the levels seen with soil pore water only. We conclude that the main toxicants present in these soil pore water samples are organic rather than metallic in nature. Organic extracts from a control standard soil (Lufa 2.2) had negligible effects on expression of these genes, and similarly several pesticides had little effect on the expression of a constitutive myo-3::GFP transgene. Both the P73 and P74 sites have been treated regularly with (undisclosed) pesticides, as permitted under EU regulations, though other (e.g. industrial) organic residues may also be present.Introduction.

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Section: The C Elegans Stress-response Networkmentioning

confidence: 99%

“…C. elegans stress-response pathways are studied using molecular biomarkers such as heat-shock proteins [2][3][4][5] or metallothioneins [6], and in terms of behavioural or life-cycle parameters such as fertility, growth, motility or feeding [7][8][9]. C. elegans is used in standard ASTMS tests for soil and water pollution [10,11], as well as for sediment testing [12].Several C. elegans stress-response pathways are influenced by the daf-2 insulin-like signalling pathway that regulates lifespan [13]. DAF-2 signalling down-regulates the FOXO transcription factor DAF-16, whose targets include the small heat-shock genes (also regulated by HSF-1) [14], the mtl-1 metallothionein gene, the daf-9 and cyp-34A9 (dod-16) P450 genes, and the mitochondrial sod-3 superoxide dismutase gene (whose product helps to inactivate reactive oxygen species, ROS).…”

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The Stress-Response Network in Animals: Proposals to Develop a Predictive Mathematical Model

Pomerai¹,

Madhamshettiwar²,

Anbalagan³

et al. 2009

TOTOXIJ

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Abstract:Increasing evidence indicates that numerous genetic pathways responding to environmental stress in animals are regulated co-ordinately as well as independently. These stress-response systems should therefore be viewed in holistic terms as a network. As such, their behaviour is susceptible to mathematical modelling using a systems biology approach. This review outlines relevant evidence and describes a newly launched project to develop just such a model using stressresponse data from multiple transgenic strains of C. elegans and D. melanogaster. We hope that our eventual model will be capable of predicting the effects of simple stressor mixtures with reasonable accuracy. To maximise the effectiveness and scope of this model, we appeal for help from colleagues to share reagents and data relevant to this project. We also present preliminary data where RNA interference has implicated the key transcription factor DAF-16 in an unexpected upregulation of cyp-34A9 reporter expression by high cadmium. STRESS RESPONSES AND MIXTURE TOXICITYIn multicellular organisms, the defensive cellular responses evoked by environmental stresses do not result from simple linear pathways, but rather from a network of interlinked pathways with multiple outputs. This makes it difficult to predict the biological effects of multiple stressors acting together, even though this is the normal situation for industrial pollution of soil or water, where several different contaminants are usually present together. There are few studies and no useful predictive models describing the molecular responses of multicellular organisms to several toxicants acting in concert. This is essentially a systems biology problem, requiring integration of complex molecular and toxicological information. Under the auspices of a Major Award from the UK-India Education and Research Initiative (UK-IERI), we intend to develop an in silico model describing the principal elements of a consensus stress response network (SRN) and its in vivo responses to single stressors, using data from two invertebrate model systems, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster. This model will be used to predict the likely SRN responses to stressor mixtures, and such predictions will then be tested experimentally in both species so that the model can be refined accordingly. Since the SRN core pathways are highly conserved among animal taxa, general features of this model should find wider application in ecotoxi-*Address correspondence to this author at the School of Biology, Nottingham University, Nottingham NG7 2RD, UK; Tel: (0044) 115 951 3250; Fax: (0044) 115 951 3251; E-mail: david.depomerai@nottingham.ac.uk cology. The dynamic aspects of this model, and in particular its ability to integrate multiple toxicant inputs for predicting likely SRN outputs, distinguish this from a simple map of the regulatory gene-circuits comprising the SRN. THE C. ELEGANS STRESS-RESPONSE NETWORKThe free-living soil nematode, C. elegans, is particularly suitable fo...

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Ecotoxicological Assessment of Aquatic Sediments With Caenorhabditis Elegans (Nematoda)—a Method for Testing Liquid Medium and Whole-Sediment Samples

Cited by 67 publications

References 32 publications

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Sediment contact tests as a tool for the assessment of sediment quality in German waters

Use of transgenic GFP reporter strains of the nematode Caenorhabditis elegans to investigate the patterns of stress responses induced by pesticides and by organic extracts from agricultural soils

The Stress-Response Network in Animals: Proposals to Develop a Predictive Mathematical Model

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